Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.)

Plant Molecular Biology

Volumn 39, Issue 2, 1999, Pages 199-208

  Ellard Ivey, Mary ^a,b   Hopkins, Roswitha B ^a   White, T J ^a   Lomax, Terri L ^a  

^a OREGON STATE UNIVERSITY   (United States)

^b PACIFIC LUTHERAN UNIVERSITY   (United States)

Author keywords

Calcium dependent protein kinase; Membrane association; Myristoylation; Zucchini

Indexed keywords

AMINO ACID SEQUENCE; CALCIUM PROTEIN KINASE; CDNA LIBRARY; CLONING; DNA ISOLATION; GENE EXPRESSION; LIGHT HARVESTING COMPLEX; MOLECULAR GENETICS; MUTAGENESIS; MYRISTYLATION; NORTHERN BLOTTING; PROTEIN ANALYSIS; PROTEIN TRANSLATION; RNA SPLICING; TRANSCRIPTION INITIATION;

NON-PROGRAMMATIC;

AMINO ACID SEQUENCE; ARABIDOPSIS PROTEINS; CALCIUM-BINDING PROTEINS; CATALYTIC DOMAIN; CELL MEMBRANE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PLANT; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MYRISTIC ACID; PLANT PROTEINS; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; PROTEIN KINASES; RNA, MESSENGER; VEGETABLES;

ARABIDOPSIS THALIANA; CUCURBITA PEPO; CUCURBITA PEPO VAR. MELOPEPO; EMBRYOPHYTA; PEPO;

EID: 0032999165     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006125918023     Document Type: Article

References (53)

1. Abo-el Saad M, Wu R: A rice calcium-dependent protein kinase is induced by gibberellin. Plant Physiol 108: 77-793 (1995).
2. Allan AC, Fricker MD, Ward J, Beale M, Trewavas AJ: Caged ABA-induced calcium transients in guard cells of Commelina communis are dependent on previous growth temperature. Plant Cell 6: 1319-1328 (1994).
3. Botella JR, Arteca JM, Somadevilla M, Arteca RN: Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata). Plant Mol Biol 30: 1129-1137 (1996).
4. Breviario D, Morello L, Giani S: Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases. Plant Mol Biol 27: 953-967 (1995).
5. Bush DS: Calcium regulation in plant cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol 46: 95-122 (1995).
6. Bush DS: Regulation of cytosolic calcium in plants. Plant Physiol 103: 7-13 (1993).
7. Casey PJ: Protein lipidation in cell signaling. Science 268: 221-225 (1995).
8. Chomczynski P, Sacci N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159 (1987).
9. Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995 (1984).
10. 2 terminus of retinal recoverin is acylated by a small family of fatty acids. J Biol Chem 267: 16033-16036 (1992).
11. Dizhoor AM, Ray S, Kumar S, Niemi G, Spencer M, Brolley D, Walsh KA, Philipov PP, Hurley JB, Stryer L: Recoverin: a calcium sensitive activator of retinal rod guanylate cyclase. Science 251: 915-918 (1991).
12. Estruch JJ, Kadwell W, Merlin E, Crossland L: Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase. Proc Natl Acad Sci USA 91: 8837-8841 (1994).
13. Franklin-Tong VE, Ryde JP, Read ND, Trewavas AJ, Franklin C: The self incompatibility response in Papaver rhoeas is mediated by free cytosolic calcium. Plant J 4: 163-177 (1993).
14. Furumoto T, Ogawa N, Hata S, Izui K: Plant calcium-dependent protein kinase-related kinases (CRKs) do not require calcium for their activities. FEBS Lett 396: 147-151 (1996).
15. Gilroy S, Bethke PC, Jones RL: Calcium homeostasis in plants. J Cell Sci 106: 453-462 (1993).
16. Grabski S, Xie XG, Holland JF, Schindler M: Lipids trigger changes in the elasticity of the cytoskeleton in plant cells: a cell optical displacement assay for live cell measurements. J Cell Biol 126: 713-726 (1994).
17. Hanks SK: Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family. Proc Natl Acad Sci USA 84: 388-392 (1987).
18. Hanks SK, Quinn AM, Hunter T: The protein kinases family: conserved features and deduced phylogeny of the catalytic domains. Science 24: 42-52 (1988).
19. Harmon AC, McCurdy DW: Calcium-dependent protein kinase and its possible role in regulation of the cytoskeleton. Curr Top Plant Biochem Physiol 9: 119-128 (1990).
20. Harper JF, Huang J-F, Lloyd SJ: Genetic identification of an autoinhibitor in CDPK, a protein kinase with a calmodulin-like domain. Biochemistry 33: 7267-7277 (1994).
21. Harper JF, Binder BM, Sussman MR: Calcium and lipid regulation of an Arabidopsis protein kinase expressed in Escherichia coli. Biochemistry 32: 3282-3290 (1993).
22. Harper JF, Sussman MR, Schaller GE, Putnam-Evans C, Charbonneau H, Harmon AC: A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951-954 (1991).
23. Heuckeroth RO, Towler DA, Adams SP, Glaser L, Gordon JI: 11-(Ethylthio)undecanoic acid. A myristic acid analogue of altered hydrophobicity which is functional for peptid N-myristoylation with wheat germ and yeast acyltransferase. J Biol Chem 263: 2127-2133 (1988).
24. Hong Y, Takano M, Liu CM, Gasch A, Chye ML, Chua N-H: Expression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana. Plant Mol Biol 30: 1259-1275 (1996).
25. Hrabak EM, Dickmann LJ, Satterlee JS, Sussman MR: Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana. Plant Mol Biol 31: 405-412 (1996).
26. 2+-dependent protein kinase involves intramolecular binding of a calmodulin-like regulatory domain. Biochemistry 35: 13222-13230 (1996).
27. Johnson DR, Bhatnager RS, Knoll LJ, Gordon JI: Genetic and biochemical studies of protein N-myristoylation. Annu Rev Biochem 63: 869-914 (1994).
28. Kawasaki T, Hayashida N, Shinozaki K, Shimada H: The gene encoding a calcium-dependent protein kinase located near the sbe1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds. Gene 129: 183-189 (1993).
29. Kluais E, Polya GM: Localization, solubilization, and characterization of plant membrane-associated protein kinases. Plant Physiol 88: 164-171 (1988).
30. Lindzen E, Choi JH: A carrot cDNA encoding an atypical protein kinase homologous to plant calcium-dependent protein kinases. Plant Mol Biol 28: 785-797 (1995).
31. Malho R, Read ND, Pais MS, Trewavas AJ: Role of cytosolic free calcium in the reorientation of pollen tube growth. Plant J 5: 331-341 (1994).
32. Mochly-Rosen D: Localization of protein kinases by anchoring proteins: a theme in signal transduction. Science 268: 247-251 (1995).
33. Putnam-Evans CL, Harmon AC, Cormier MJ: Purification and characterization of a novel calcium-dependent protein kinase from soybean. Biochemistry 29: 2488-2495 (1990).
34. Putnam-Evans CL, Harmon AC, Palevitz BA, Fechheimer M, Cormier MJ: Calcium-dependent protein kinase is localized with F-actin in plant cells. Cell Motil Cytoskel 12: 12-22 (1989).
35. Risinger MA, Korsgren C, Cohen CM: Role of N-myristylation in targeting of band 4.2 (pallidin) in nonerythroid cells. Exp Cell Res 229: 421-431 (1996).
36. Roberts DM, Harmon AC: Calcium-modulated proteins: targets of intracellular calcium signals in higher plants. Annu Rev Plant Physiol Plant Mo] Biol 43: 375-414 (1992).
37. Rogers SO, Bendich AJ: Extraction of DNA from milligram amounts of fresh, herbarium, and mummified plant tissues. Plant Mol Biol 5: 69-76 (1985).
38. Rommens CMT, Salmeron JM, Baulcombe DC, Staskawicz BJ: Use of a gene expression system based in Potato Virus X to rapidly identify and characterize a tomato pto homolog that controls fenthion sensitivity. Plant Cell 7: 249-257 (1995).
39. Sambrook J, Maniatis E, Fritsch T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1988).
40. +-ATPase of oat roots by calcium-stimulated protein kinases. Planta 173: 509-518 (1988).
41. Schultz AM, Oroszlan S: In vivo modification of retroviral gag gene-encoded polyproteins by myristic acid. J Virol 46: 355-361 (1993).
42. Shacklock P, Read ND, Trewavas AJ: Cytosolic free calcium mediates red light-induced photomorphogenesis. Nature 358: 753-755 (1992).
43. Stephenson M, Ryals PE, Thompson Jr GA: Fatty acid acylated proteins of the halotolerant alga, Dunaliella salina. Plant Physiol 90: 549-552 (1989).
44. Stone JM, Walker JC: Plant protein kinase families and signal transduction. Plant Physiol 108: 451-457 (1995).
45. Suen K-L, Choi JH: Isolation and sequence analysis of a cDNA clone for a carrot calcium-dependent protein kinase: homology to calcium/calmodulin-dependent protein kinases and to calmodulin. Plant Mol Biol 17: 581-590 (1991).
46. Tanaka T, Ames JB, Harvey TS, Stryer L, Ikura M: Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state. Nature 376: 444-447 (1995).
47. Towler DA, Adams SP, Eubanks SR, Towery DS, Jackson-Machelski E, Glaser L, Gordon JI: Myristoyl CoA:Protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities. J Biol Chem 262: 1784-1790 (1988).
48. Towler DA, Adams SP, Eubanks SR, Towery DS, Jackson-Machelski E, Glaser L, Gordon JI: Purification and characterization of yeast myristoyl CoA:Protein N-myristoyltransferase. Proc Natl Acad Sci USA 84: 2708-2712 (1987).
49. Trewavas A, Knight M: Mechanical signaling, calcium and plant form. Plant Mol Biol 26: 1329-1341 (1994).
50. +2-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana. Mol Gen Genet 244: 331-340 (1994).
51. c-scr from the plasma membrane to the cytosol after stimulation by platelet-derived growth factor. J Biol Chem 268: 19552-19558 (1993).
52. Weinberg RA, McWherter CA, Freeman SK, Wood DC, Gordon JI, Lee SC: Genetic studies reveal that myristoyl-CoA:Protein N-myristoyltransferase is an essential enzyme in Candida albicans. Mol Microbiol 16: 241-250 (1995).
53. Verhey SD, Gaiser JC, Lomax TL: Protein kinases in zucchini: characterization of calcium-requiring plasma membrane kinases. Plant Physiol 103: 413-419 (1993).