Purification of phycobiliproteins from Nostoc sp. by aminohexyl-Sepharose chromatography

Journal of Biotechnology

Volumn 69, Issue 1, 1999, Pages 69-73

  Tchernov, A A ^a   Minkova, K M ^b   Houbavenska, N B ^b   Kovacheva, N G ^c  

^a NATIONAL CENTER OF INFECTIOUS AND PARASITIC DISEASES   (Bulgaria)

^b INSTITUTE OF PHYSIOLOGY   (Bulgaria)

^c UNIVERSITY OF SOFIA   (Bulgaria)

Author keywords

Aminohexyl Sepharose chromatography; Nostoc sp.; Phycobiliproteins

Indexed keywords

ALGAE; AMMONIUM COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; PRECIPITATION (CHEMICAL); PURIFICATION;

AMINOHEXYL-SEPHAROSE CHROMATOGRAPHY; AMMONIUM SULFATE; PHYCOBILIPROTEINS;

PROTEINS;

PHYCOBILIPROTEIN; PHYCOCYANIN; PHYCOERYTHRIN; SEPHAROSE; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS; CHROMATOGRAPHY; FRACTIONATION; IMMUNOELECTROPHORESIS; NONHUMAN; NOSTOC; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

NOSTOC;

EID: 0032997745     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00206-5     Document Type: Article

References (20)

1. Bermejo R., Talavera E.M., Alvarez-Pez J.M., Orte C.J. Chromatographic purification of biliproteins from Spirulina platensis. High-performance liquid chromatographic separation of their α and β subunits. J. Chromatogr. A. 778:1997;441-450.
2. Cambiaso C.L., Goffinet A., Vaerman J.P., Heremans J.F. Glutaraldehyde-activated aminohexyl derivative of Sepharose 4B as a new versatile immunoabsorbent. Immunochemistry. 12:1975;273-278.
3. Duerring M., Schmidt G.B., Huber R. Isolation, crystallization, crystal structure analysis and refinement of constitutive C-phycocyanin from the chromatically adapting cyanobacterium Fremyella diplosiphon at 1.66 A resolution. J. Mol. Biol. 217:1991;577-592.
4. Ficner R., Lobeck K., Schmidt G., Huber R. Isolation, crystallization, crystal structure analysis and refinement of B-phycoerythrin from the red alga Porphyridium sordidum at 2.2 A resolution. J. Mol. Biol. 228:1992;935-950.
5. Glazer A.N. Phycobiliproteins - a family of valuable, widely used fluorophores. J. Appl. Phycol. 6:1994;105-112.
6. ,un melange de proteins; application au serum sanguin. Biochim. Biophys. Acta (Amst.). 10:1953;193-194.
7. Grabowski J., Gantt E. Photophysical properties of phycobiliproteins from phycobilisomes: fluorescence lifetimes, quantum yields, and polarization spectra. Photochem. Photobiol. 28:1978;39-45.
8. Gray B.H., Gantt E. Spectral properties of phycobilisomes and phycobiliproteins from the blue-green alga Nostoc sp. Photochem. Photobiol. 21:1975;121-128.
9. Kochert G. Carbohydrate determination by the phenol-sulfuric acid method. Hellebust J.A., Craigie J.S. Handbook of Phycological Methods, Physiological and Biochemical Methods. 1978;95-97 Cambridge University Press, Cambridge.
10. Lowry H.O., Rosenbrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
11. Mihova S.G., Georgiev D.I., Minkova K.M., Tchernov A.A. Phycobiliproteins in Rhodella reticulata and photoregulatory effects on their content. J. Biotechnol. 48:1996;251-257.
12. Moreno J., Rodriguez H., Vargas M.A., Rivas J., Guerrero M.G. Nitrogen-fixing cyanobacteria as source of phycobiliprotein pigments. Composition and growth performance of ten filamentous heterocystous strains. J. Appl. Phycol. 7:1995;17-23.
13. Rabier J., Vijayalakshmi M., Rudiger W. Affinity of phycocyanin chromopeptides to histidyl-Sepharose gels: A model for histidine-tetrapyrrol-interactions in biliproteins. Z. Naturforsch. 38:1983;230-236.
14. Rabier J., Vijayalakshmi M., Lambert C. Affinity of phycoerythrin chromopeptides to histidyl, tyrosyl and tryptophyl Sepharose gels. J. Chromatogr. 295:1984;215-219.
15. Rodriguez H., Rivas J., Guerrero M.G., Losada M. Enhancement of phycobiliprotein production in nitrogen-fixing cyanobacteria. J. Biotechnol. 20:1991;263-270.
16. Siegelman H.W., Kycia J.H. Algal biliproteins. Hellebust J.A., Craigie J.S. Handbook of Phycological Methods, Physiological and Biochemical Methods. 1978;71-79 Cambridge University Press, Cambridge.
17. Tchernov A.A., Minkova K.M., Georgiev D.I., Houbavenska N.B. Method for B-phycoerythrin purification from Porphyridium cruentum. Biotechnol. Tech. 7:1993;853-858.
18. Yon R.J. Chromatography of lipophilic proteins on adsorbents containing mixed hydrophobic and ionic groups. Biochem. J. 126:1972;765-767.
19. Yon R.J., Simmonds R.J. Protein chromatography on adsorbents with hydrophobic and ionic groups. Some properties of N-(3-carboxypropionyl)-aminodecyl-Sepharose and its interaction with wheat-germ aspartate transcarbamoylase. Biochem. J. 151:1975;281-290.
20. Zubjitskii, Iu., 1960. Effective method for preparation of high titre precipitin sera for luminiscent antibodies application, Laboratornoe delo 6, 8-11 (in Russian).