Protein glycosylation mutants of procyclic Trypanosoma brucei: Defects in the asparagine-glycosglation pathway

Glycobiology

Volumn 9, Issue 2, 1999, Pages 181-190

  Hwa, Kuo Yuan ^a,b   Acosta Serrano, Alvaro ^a   Khoo, Kay Hooi ^b   Pearson, Terry W ^c   Englund, Paul T ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^c NONE

Author keywords

Concanavalin A; Glycoprotein; Glycosylation mutant; Trypanosoma brucei

Indexed keywords

ASPARAGINE; CONCANAVALIN A; LECTIN; MEMBRANE PROTEIN; WHEAT GERM AGGLUTININ;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CELL AGGLUTINATION; CELL KILLING; CELL MUTANT; CONTROLLED STUDY; FLAGELLUM; NONHUMAN; PARASITE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN GLYCOSYLATION; TRYPANOSOMA BRUCEI;

ANIMALS; ASPARAGINE; CARBOHYDRATE SEQUENCE; CONCANAVALIN A; DRUG RESISTANCE; FLAGELLA; GLYCOSYLATION; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTOZOAN PROTEINS; SPECTROMETRY, MASS, FAST ATOM BOMBARDMENT; TRYPANOSOMA BRUCEI BRUCEI;

TRITICUM AESTIVUM; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0032997558     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.2.181     Document Type: Article

References (31)

1. Bangs, J.D., Andrews, N.W., Hart, G.W. and Englund, P.T. (1986) Posttranslational modification and intracellular transport of a trypanosome variant surface glycoprotein. J. Cell Biol., 103, 255-263.
2. Brun, R. and Shonenberger, M. (1979) Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Tropica, 36, 289-292.
3. Butikofer, P., Ruepp, S., Boschung, M. and Roditi, I. (1997) 'GPEET' procyclin is the major surface protein of procyclic culture forms of Trypanosoma brucei brucei strain 427. Biochem. J., 326, 415-423.
4. Carver, J.P. and Brisson, J.-R. (1984) The three-dimensional structure of N-linked oligosaccharides. In Ginsburg, V. and Robbins, P.W. (eds.), Biology of Carbohydrates, Vol. 2. John Wiley, New York, pp. 289-331.
5. Clayton, C.E. and Mowatt, M.R. (1989) The procyclic acidic repetitive proteins of Trypanosoma brucei. Purification and post-translational modification. J. Biol. Chem., 264, 15088-15093.
6. Cunningham, I. (1977) New culture medium for maintenance of tsetse tissues and growth of trypanosomatids. J. Protozool., 24, 325-329.
7. Dell, A., Khoo, K.-H., Panico, M., McDowell, R.A., Etienne, A.T., Reason, A.J. and Morris, H.R. (1993) In Fukuda, M. and Kobata, A. (eds.), Glycobiology. A Practical Approach. Oxford University Press, New York, pp. 187-222.
8. Engstler, M., Reuter, G. and Schauer, R. (1993) The developmentally regulated trans-sialidase from Trypanosoma brucei sialylates the procyclic acidic repetitive protein. Mol. Biochem. Parasitol., 61, 1-13.
9. Ferguson, M.A.J., Murray, P., Rutherford, H. and McConville, M.J. (1993) A simple purification of procyclic acidic repetitive protein and demonstration of a sialylated glycosyl-phosphatidylinositol membrane anchor. Biochem. J., 291, 51-55.
10. Field, M.C., Menon, A.K. and Cross, G.A.M. (1991) Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. Identification of a candidate biosynthetic precursor of the glycosylphosphatidylinositol anchor of the major procyclic stage surface glycoprotein. J. Biol. Chem., 266, 8392-8400.
11. Field, M.C., Menon, A.K. and Cross, G.A.M. (1991) A glycosylphosphatidylinositol protein anchor from procyclic stage Trypanosoma brucei: lipid structure and biosynthesis. EMBO J., 10, 2731-2739.
12. Fraser, A.R., Hemperly, J.J., Wang, J.L. and Edelman, G.M. (1976) Monovalent derivatives of concanavalin A. Proc. Natl. Acad. Sci. USA, 73, 790-794.
13. Goldstein, I.J. and Poretz, R.D. (1986) Isolation, physicochemical characterization, and carbohydrate-binding specificity of lectins. In Liener, I.E., Sharon, N. and Goldstein, I.J. (eds.), The Lectins: Properties, Functions, and Applications in Biology and Medicine. Academic Press, Orlando, pp. 33-247.
14. Hunt, L.A. (1982) Lectin affinity chromatography of glycopeptides and oligosaccharides from normal and lectin-resistant Chinese-hamster ovary cells. Biochem. J., 205, 623-630.
15. King, D.L. and Turco, S.J. (1988) A ricin agglutinin-resistant clone of Leishmania donovani deficient in lipophosphoglycan. Mol. Biochem. Parasitol., 28, 285-293.
16. McConville, M.J. and Ferguson, M.A.J. (1993) The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes. Biochem. J., 294, 305-324.
17. Mowatt, M.R. and Clayton, C.E. (1987) Developmental regulation of a novel repetitive protein of Trypanosoma brucei. Mol. Cell Biol., 7, 2838-2844.
18. Mowatt, M.R., Wisdom, G.S. and Clayton, C.E. (1989) Variation of tandem repeats in the developmentally regulated procyclic acidic repetitive proteins of Trypanosoma brucei. Mol. Cell Biol., 9, 1332-1335.
19. Nozaki, T., Haynes, P.A. and Cross, G.A.M. (1996) Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein. Mol. Biochem. Parasitol., 82, 245-255.
20. Pontes de Carvalho, L.C., Tomlinson, S., Vandekerckhove, F., Bienen, E.J., Clarkson, A-B., Jiang, M.-S., Hart, G.W. and Nussenzweig, V. (1993) Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. J. Exp. Med., 177, 465-474.
21. Reinhold, B.B., Hauer, C.R., Plummer, T.H. and Reinhold, V.N. (1995) Detailed structural analysis of a novel, specific O-linked glycan from the prokaryote Flavobacterium meningosepticum. J. Biol. Chem., 270, 13197-13203.
22. Richardson, J.P., Beecroft, R.P., Tolson, D.L., Liu, M.K. and Pearson, T.W. (1988) Procyclin: an unusual immunodominant glycoprotein surface antigen from the procyclic stage of African trypanosomes. Mol. Biochem. Parasitol., 31, 203-216.
23. Robbins, P.W. (1994) The ALG (asparagine linked glycosylation) genes. In Rothblatt, J., Novick, P. and Stevens, T. (eds.), Guidebook to the Secretory Pathway. Oxford University Press, Oxford, pp. 57-59.
24. Roditi, I., Carrington, M. and Tumer, M. (1987) Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei. Nature, 325, 272-274.
25. Roditi, I., Schwarz, H., Pearson, T.W., Beecroft, R.P., Liu, M.K., Richardson, J.P., Buhring, H.J., Pleiss, J., Bulow, R., Williams, R.O. and Overath, P. (1989) Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei. J. Cell. Biol., 108, 737-746.
26. Ruepp, S., Furger, A., Kurath, U., Renggli, C.K., Hemphill, A., Brun, R. and Roditi, I. (1997) Survival of Trypanosoma brucei in the tsetse fly is enhanced by the expression of specific forms of procyclin. J. Cell Biol., 137, 1369-1379.
27. Sommer, J.M., Hua, S., Li, F., Gottesdiener, K.M. and Wang, C.C. (1996) Cloning by functional complementation in Trypanosoma brucei. Mol. Biochem. Parasitol., 76, 83-89.
28. Stanley, P. and Ioffe, E. (1995) Glycosyltransferase mutants: key to new insights in glycobiology. FASEB J., 9, 1436-1444.
29. Treumann, A., Zitzmann, N., Hulsmeier, A., Prescott, A.R., Almond, A., Sheehan, J. and Ferguson, M.A. (1997) Structural characterisation of two forms of procyclic acidic repetitive protein expressed by procyclic forms of Trypanosoma brucei. J. Mol. Biol., 269, 529-547.
30. Turco, S.J. and Descoteaux, A. (1992) The lipophosphoglycan of Leishmania parasites. Annu. Rev. Microbiol., 46, 65-94.
31. Welburn, S.C., Dale, C., Ellis, D., Beecroft, R. and Pearson, T.W. (1996) Apoptosis in procyclic Trypanosoma brucei rhodesiense in vitro. Cell Death Differ., 3, 229-236.