Compromised concentrations of ascorbate in fluid lining the respiratory tract in human subjects after exposure to ozone

Occupational and Environmental Medicine

Volumn 56, Issue 7, 1999, Pages 473-481

  Mudway, Ian S ^a   Krishna, Mamidipudi T ^b   Frew, Anthony J ^b   MacLeod, Dominic ^b   Sandstrom, Thomas ^c   Holgate, Stephen T ^b   Kelly, Frank J ^a  

^a ST THOMAS HOSPITAL   (United Kingdom)

^b UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^c UMEÅ UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Antioxidant; Ascorbate; Bronchoalveolar lavage; Ozone

Indexed keywords

ANTIOXIDANT; ASCORBIC ACID; BIOLOGICAL MARKER; MYELOPEROXIDASE; OZONE;

ARTICLE; BLOOD LEVEL; BLOOD SAMPLING; CELL COUNT; CELL DAMAGE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CROSSOVER PROCEDURE; DOUBLE BLIND PROCEDURE; ENVIRONMENTAL EXPOSURE; EOSINOPHIL; EPITHELIUM CELL; EXERCISE; FORCED EXPIRATORY VOLUME; HUMAN; HUMAN EXPERIMENT; INFLAMMATION; LEUKOCYTE DIFFERENTIAL COUNT; LUNG FUNCTION TEST; LUNG LAVAGE; LYMPHOCYTE; MACROPHAGE; NEUTROPHIL; NORMAL HUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; PROVOCATION TEST; RESPIRATORY SYSTEM; REST; STATISTICAL ANALYSIS;

EID: 0032996692     PISSN: 13510711     EISSN: None     Source Type: Journal    
DOI: 10.1136/oem.56.7.473     Document Type: Article

References (35)

1. 1 Bascom R, Bromberg PA, Costa DA, et al. Health effects of outdoor air pollution. Am J Respir Crit Care Med 1996;153:3-50.
2. 2 Department of Health Advisory Group on the Medical Aspects of Air Pollution. First report: ozone. London: Department of Health, The Stationery Office, 1991.
3. 3 Pryor WA. Mechanisms of radical formation from reactions of ozone with target molecules in the lung. Free Radic Biol Med 1994;17:451-65.
4. 4 Pryor WA. Ozone in all its reactive splendor. J Lab Clin Med 1993;122:483-6.
5. 5 Postlethwait EM, Langford SD, Bidani A. Determinants of inhaled ozone absorption in isolated rat lungs. Toxicol Appl Pharmacol 1994;125:77-89.
6. 6 Pryor WA, Squadrito GL, Friedman M. The cascade mechanism to explain ozone toxicity: the role of lipid ozonation products. Free Raid Biol Med 1095;19:935-41.
7. 7 Langford SD, Bidani A, Postlethwait EM. Ozone-reactive absorption by pulmonary epithelial lining fluid constituents, Toxicol Pharmacol 1995;132:122-30.
8. 8 Kelly FJ, Mudway I, Krishna MT, et al. The free radical basis of air pollution: focus on nzone. Respir Med 1995;89:647-56.
9. 9 Schelegle ES, Siefkin AD, McDonald RJ. Time course of ozone-induced neutrophilia in normal humans. Am Rev Respir Dis 1991;143:1353-8.
10. 10 Barnes PJ. Reactive oxygen species and airway inflammation. Free Radic Biol Med 1900;9:235-43.
11. 11 Mudway I, Kelly FJ, Khrishna T, et al. Upper airways loss of uric acid during exposure to ozone [abstract]. Eur J Respir Dis 1996;9:415s.
12. 12 Blomberg A, Mudway I, Frew A, et al. Dose-dependent antioxidant and cellular responses in the airways of human subjects exposed to ozone [abstract]. Eur Respir J 1996;9:147s.
13. 13 Baker MA, Cerniglia GJ, Zaman A, Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 1990;190:360-5.
14. 14 Iriyama K, Yoshiura M, Iwamoto T, et al. Simultaneous determination of uric and ascorbic acids in human scrum by reversed-phase high-performance liquid chromatography with electrochemical detection. Anal Biochem 1984;141:238-43.
15. 15 Bieri JG, Tolliver TJ, Catignani GL. Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am J Clin Nutr 1979;32:2143-9.
16. 16 Smith PK, Krohn RI, Hermanson GT, et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985; 150:76-85.
17. 17 Waynor DD, Barton GW, Ingold KU, et al. The relative contributions of vitamin E, urate, ascorbate, and proteins to the total peroxyl radical trapping antioxidant activity of human blood plasma. Biochem Biophys Acta 1987;924:408-19.
18. 18 Chirico S. High-performance liquid chromatography-based thiobarbituric acid tests. Methods Enzymol 1994;233:314-8.
19. 19 Kelly FJ, Blumberg A, Frew AJ, et al. Antioxidant kinetics in lung lining fluid following exposure of human to nitrogen dioxide Am J Respir Crit Care Med 1996;154:1700-5.
20. 20 Reznick AZ, Packer L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 1994;233:357-63.
21. 21 Slade R, Crissman K, Norwood J, et al. Comparison of antioxidant substances in bronchoalveolar lavage cells and fluid from humans, guinea pigs, and rats. Exp Lung Res 1993;19:469-84.
22. 22 Mudway IS, Housley D, Eccles R, et al. Differential depletion of human respiratory tract antioxidants in response to ozone challenge. Free Radic Res 1996;25:499 513.
23. 23 Giamalva D, Church D, Pryor W. A comparison of the rates of ozonation of biological antioxidants and linoleate esters. Biochem Biophys Res Commun 1985;133:773-9.
24. 24 Mudway I, Kelly FJ. Consumption kinetics of water soluble antioxidants in lung lining fluid by ozone [abstract]. J Free Radic Biol Med 1994;9:M/O15.
25. 25 Frei B. Ascorbic acid protects lipids in human plasma and low density lipoprotein against oxidative damage. Am J Clin Nutr 1991;54:1113S-8S.
26. 26 Stocker R, Frei B. Endogenous antioxidant defences in human blood plasma. In: Sies H, ed. Oxidative stress: oxidants and antioxidants. San Diego: Academic Press, 1991;213-43.
27. 27 Frei B, Forte TM, Ames BN, at al. Gas phase oxidants of cigarette smoke induce lipid peroxidation and change in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid. Biochem J 1994;277:133-8.
28. 28 Cross CF, Forte T, Stocker R, et al. Oxidative stress and abnormal cholesterol metabolism in patients with adult respiratory distress syndrome. J Lab Clin Med 1990;115:396-404.
29. 29 Chow CK, Thacker RR, Changchit C, et al. Lower levels of vitamin C and carotenes in plasma of cigarette smokers. J Am Coll Nutr 1986;5:305-2.
30. 30 Slade R, Highfill JW, Hatch GE. Effects of depletion of ascorbic acid or nonprotein sulfhydryls on the acute inhalation toxicity of nitrogen dioxide, ozone and phosgene. Inhalation Toxicology 1989;1;261-71.
31. 31 Wiester MJ, Tepper JS, Winsett DW, et al. Adaptation to ozone in rats and its association with ascorbic acid in the lung. Fundam Appl Toxicol 1996;31:56-64.
32. 32 Aris RM, Christian D, Hearne PQ, et al. Ozone-induced airway inflammation in human subjects as determined by airway lavage and biopsy. Am Rev Respir Dis 1993;148;1363 72.
33. 33 Koren HS, Hatch GE, Graham DE. Nasal lavage as a tool in assessing acute inflammation in response to inhaled pollutants. Toxicology 1990;60:15-25.
34. 34 Vera JC, Rivas CI, Fischbarg J, et al. Mammalian facilitated hexose transporters mediate the transport of dehydroascorbic acid. Nature 1993;364:79-82.
35. 35 Frampton MW, Morrow PF, Torres A, et al. Effects of ozone on normal and potentially sensitive human subjects. Part II: Airway inflammation and responsiveness to ozone in nonsmokers and smokers. Health Effects Institute Research Report 1997;78:39-72.