Gephyrin-like immunoreactivity is a marker for growing axons in the central nervous system of the immature rat

Developmental Neuroscience

Volumn 21, Issue 1, 1999, Pages 50-57

  Fallah, Zahra ^a   Clowry, Gavin J ^a,b  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b ROYAL VICTORIA INFIRMARY   (United Kingdom)

Author keywords

Corticospinal tract; Development; Gephyrin; Microtubules; Myelination

Indexed keywords

GEPHYRIN; MONOCLONAL ANTIBODY;

ANIMAL TISSUE; ARTICLE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; GRAY MATTER; IMMUNOELECTRON MICROSCOPY; IMMUNOREACTIVITY; NERVE FIBER GROWTH; NONHUMAN; NONMYELINATED NERVE; PRIORITY JOURNAL; PYRAMIDAL TRACT; RAT; WESTERN BLOTTING;

EID: 0032995514     PISSN: 03785866     EISSN: None     Source Type: Journal    
DOI: 10.1159/000017366     Document Type: Article

References (34)

1. Ahmad F, Pienkowski TP, Baas PW (1993): Regional differences in microtubule dynamics in the axon. J Neurosci 13:856-866.
2. Baas PW, Ahmad FJ, Pienkowski TP, Brown A, Black MM (1993): Sites of microtubule stabilisation for the axon. J Neurosci 13:2177-2185.
3. Baas PW, Black MM (1990): Individual microtubules in the axon consist of domains that differ in both composition and stability. J Cell Biol 111:495-509.
4. Becker C-M, Hoch W, Betz H (1988): Glycine receptor heterogeneity in rat spinal cord during postnatal development. EMBO J 7:3717-3726.
5. Boyne LJ, Martin K, Hockfield S, Fischer I (1995): Expression and distribution of phosphorylatcd MAPIB in growing axons of cultured hippocampal neurons. J Neurosci Res 40:439-450.
6. Brandt R, Lee G (1993): Functional organisation of microtubule-associated protein tau - Identification of regions which affect microtubule growth, nucleation, and bundle formation in vitro. J Biol Chem 68:3414-3419.
7. 3H]strychnine binding sites in rat brain. Eur J Pharmacol 126:303-307.
8. Cabot JB, Bushnell A, Alessi V, Mendell NR (1995): Postsynaptic gephyrin immunoreactivity exhibits a nearly one-to-one correspondence with gamma-aminobutyric acid-like immunogold labelled synaptic inputs to sympathetic preganglionic neurons. J Comp Neurol 356: 418-432.
9. Craig AM, Banker G, Chang W, McGrath M, Serpinskaya AS (1996): Clustering of gephyrin at GABAergic but not glutamatergic synapses in cultured rat hippocampal neurons. J Neurosci 16:3166-3177.
10. Curfs MHJM, Gribnau AAM, Dederen PJWC (1994): Selective elimination of transient corticospinal projections in the rat cervical spinal cord grey matter. Dev Brain Res 78:182-190.
11. Curtis DR, Hösli L, Johnston GAR (1968): A pharmacological study of the depression of spinal neurones by glycine and related amino acids. Exp Brain Res 6:1-18.
12. Davis JQ, McLaughlin T, Bennet V (1993): Ankyrin-binding proteins related nervous system cell adhesion molecules: Candidates to provide transmembrane and intercellular connections in adult brain. J Cell Biol 121:121-133.
13. Dodd J, Jessell TM (1988): Axon guidance and the patterning of neuronal projections in vertebrates. Science 242:4158-4168.
14. Fallah Z, Clowry GJ (1996): Localisation of gephyrin to growing axons by immunohistochemistry and a comparison between the time course of its expression and myelination in the corticospinal tract of the rat. J Anat 189:235.
15. Gorgels TGMF (1990): A quantitative analysis of axon outgrowth, axon loss and myelination in the rat pyramidal tract. Dev Brain Res 54:51-61.
16. Gorgels TGMF, van Lookcren Campagne M, Oestereicher AM, Gribnau AMM, Gispen WH (1989): B-50/GAP 43 is localised at the cytoplasmic side of the plasma membrane in developing and adult rat pyramidal tract. J Neurosci 9:3681-3689.
17. Joosten EAJ (1994): Developmental expression of N-CAM epitopes in the rat spinal cord during corticospinal axon outgrowth and target innervation. Dev Brain Res 78:226-236.
18. Joosten EAJ, Gribnau AAM, Gorgels TGMF (1990): Immunoelectron microscopic localisation of the cell adhesion molecule L1 in developing rat pyramidal tract. Neuroscience 38: 675-686.
19. Keilhauer G, Faissner A, Schachner M (1985): Differential inhibition of neurone-neurone, neurone-astrocyte and astrocyte-astrocyte adhesion of L1, L2 and N-CAM antibodies. Nature 316:728-730.
20. Kirsch J, Betz H (1993): Widespread expression of gephyrin, a putative glycine receptor-tubulin linking protein, in rat brain. Brain Res 621: 301-310.
21. Kirsch J, Betz H (1995): The postsynaptic localisation of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton. J Neurosci 15:4148-4156.
22. Kirsch J, Langosch D, Prior P, Littauer UZ, Schmitt B, Betz H (1991): The 93 kDa glycine receptor associated protein binds to tubulin. J Biol Chem 266:22242-22245.
23. Kirsch J, Malosio M-L, Wolters I, Betz H (1993a): Distribution of gephyrin transcripts in the adult and developing rat brain. Eur J Neurosci 5:1109-1117.
24. Kirsch J, Wolters I, Triller A, Betz H (1993b): Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal neurones. Nature 366:745-748.
25. Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
26. Langosch D, Thomas L, Betz H (1988): Conserved structure of ligand gated ion channels: The inhibitory glycine receptor is a pentamer. Proc Natl Acad Sci USA 85:7394-7398.
27. Prior P, Schmidt B, Grenningloh G, Pribilla I, Multhaup G, Beyreuther K, Maulet Y, Werner P, Langosch D, Kirsch J, Betz H (1992): Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron 8:1161-1170.
28. Pfeiffer F, Simler R, Grenningloh G, Betz H (1984): Monoclonal antibodies and peptide mapping reveal structural similarities between the subunits of the glycine receptor of rat spinal cord. Proc Natl Acad Sci USA 81:7224-7227.
29. A-receptor subunits in the rat retina. J Comp Neurol 357:1-14.
30. Schmitt B, Knaus P, Becker CM, Betz H (1987): The Mr 93,000 polypeptide of the postsynaptic glycine receptor complex is a peripheral membrane protein. Biochemistry 26:805-811.
31. Schmued LC (1990): A rapid, sensitive histochemical stain for myelin in frozen brain sections. J Histochem Cytochem 38:717-720.
32. Stanfield BB (1992): The development of the corticospinal projection. Prog Neurobiol 38:169-202.
33. Todd AJ, Spike RC, Chong D, Neilson M (1995): The relationship between glycine and gephyrin in synapses of the rat spinal cord. Eur J Neurosci 7:1-11.
34. Van den Pol AN, Gores T (1988): Glycine and glycine receptor immunoreactivity in brain and spinal cord. J Neurosci 8:472-492.