A CCD-based system for the detection of DNA in electrophoresis gels by UV absorption

Physics in Medicine and Biology

Volumn 44, Issue 6, 1999, Pages 1529-1541

  Mahon, Alex R ^a,b   MacDonald, John H ^c   Ott, Robert J ^a   Mainwood, Alison ^c  

^a INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ETHIDIUM BROMIDE;

ACCURACY; ARTICLE; DEVICE; DNA DETERMINATION; GEL ELECTROPHORESIS; PRIORITY JOURNAL; QUANTITATIVE ASSAY; TECHNIQUE; ULTRAVIOLET SPECTROSCOPY;

EID: 0032994925     PISSN: 00319155     EISSN: None     Source Type: Journal    
DOI: 10.1088/0031-9155/44/6/307     Document Type: Article

References (15)

1. Andrews A T 1988 Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications (New York: Oxford University Press)
2. Chan K C, Koutny L B and Yeung E S 1991 On-line detection of DNA in gel electrophoresis by ultraviolet absorption utilizing a charge-coupled device imaging system Anal. Chem. 63 746-50
3. Clarke P, Chi-Lin H and Wilcox G 1982 Ultraviolet imaging: a simple method for detecting nucleic acids in preparative gels Anal. Biochem. 124 88-91
4. El Rassi Z 1994 Capillary electrophoresis of carbohydrates Adv. Chromatogr. 34 177-250
5. Hartley J L and Gregori T J 1981 Cloning multiple copies of a DNA segment Gene 13 347-53
6. Hassur S M and Whitlock H W 1974 UV shadowing - a new and convenient method for the location of ultraviolet-absorbing species in polyacrylamide gels Anal. Biochem. 59 162-4
7. Hu A and Hartley J L 1992 A new ladder for sizing DNA fragments Focus 14 93
8. Kiseleva M N, Zarochentseva Y P and Dodonova N Y 1975 Absorption spectra of nucleic acids and related compounds in the spectral region 120-280 nm Biofizika 20 561-5
9. MacDonald J 1998 PhD Thesis Institute of Cancer Research, University of London
10. MacDonald J H, Mahon A R, Allers L A, Hassard J F, Mainwood A and Ott R J 1997a A CCD system for UV imaging of biomolecules Nucl. Instrum. Methods A 392 227-32
11. MacDonald J H, Wells K, Reader A J and Ott R J 1997b A CCD-based tissue imaging system Nucl. Instrum. Methods A 392 220-6
12. McGregor D A and Yeung E S 1992 Interactive control of pulsed field gel electrophoresis via real time monitoring Anal. Chem. 64 1-6
13. Naletto G, Tondello G, Bonanno G, Dibenedetto R and Scuderi S 1994 Response analysis in the 300-angstrom to 2500-angstrom spectral range of ultraviolet-enhanced charge coupled devices Opt. Eng. J. 33 2544-552
14. Sambrook J, Fritsch E F and Maniatis T 1989 Molecular Cloning: A Laboratory Manual 2nd edn (New York: Cold Spring Harbour Laboratory Press)
15. Sharp P A, Sugden B and Sambrook J 1973 Detection of two restriction endonuclease activities in Haemophilius parainfluenzae using analytical agarose-ethidium bromide electrophoresis Biochemistry 12 3055