Catalytic role of the active site histidine of porcine pancreatic phospholipase A2 probed by the variants H48Q, H48N and H48K

Protein Engineering

Volumn 12, Issue 6, 1999, Pages 497-503

  Janssen, Marcel J W ^a   Van De Wiel, Wendy A E C ^a   Beiboer, Sigrid H W ^a   Van Kampen, Muriel D ^a   Verheij, Hubertus M ^a   Slotboom, Arend J ^a   Egmond, Maarten R ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Active site histidine; Conformational stability; Inhibitor binding; Porcine pancreatic phospholipase A2; Site directed mutagenesis

Indexed keywords

ASPARAGINE; GLUTAMINE; GLYCEROPHOSPHOLIPID; HISTIDINE; LYSINE; PHOSPHOLIPASE A2;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; HYDROGEN BOND; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SWINE;

EID: 0032993357     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.6.497     Document Type: Article

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