The protein phosphatase inhibitor cantharidin alters vascular endothelial cell permeability

Journal of Pharmacology and Experimental Therapeutics

Volumn 289, Issue 3, 1999, Pages 1480-1486

  Knapp, Jörg ^b   Bokník, Peter ^c   Lüss, Iva ^c   Huke, Sabine ^c   Linck, Bettina ^c   Lüss, Hartmut ^c   Müller, Frank U ^c   Müller, Thorsten ^c   Nacke, Peter ^c   Noll, Thomas ^a   Piper, Hans M ^a   Schmitz, Wilhelm ^c   Vahlensieck, Ute ^c   Neumann, Joachim ^c  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

CANTHARIDIN; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CONCENTRATION RESPONSE; DOSE RESPONSE; DRUG EFFECT; ENDOTHELIUM CELL; ENZYME ACTIVE SITE; ENZYME INHIBITION; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

ANIMALS; AORTA; CANTHARIDIN; CATTLE; CELL MEMBRANE PERMEABILITY; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; ENZYME INHIBITORS; KINETICS; MYOSIN LIGHT CHAINS; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOPROTEINS; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SERUM ALBUMIN, BOVINE; TRANSCRIPTION, GENETIC;

EID: 0032992597     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Barker HM, Brewis ND, Street AJ, Spurr NK and Cohen PTW (1994) Three genes for protein phosphatase 1 map to different human chromosomes: Sequence, expression and gene localization of protein serine/threonine phosphatase 1 beta (PPP 1CB). Biochim Biophys Acta 1220:212-218.
2. Barker HM, Jones TA, Da Cruze Silva EF, Spurr NK, Sheer D and Cohen PTW (1990) Localization of the gene encoding a type 1 protein phosphatase catalytic subunit to human chromosome band 11q13. Genomics 7:159-166.
3. Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
4. Cohen P (1989) The structure and regulation of protein phosphatases. Annu Rev Biochem 58:453-508.
5. DePaoli-Roach AA, Park I-N, Cerovsky V, Csortos C, Durbin SD, Kuntz MJ, Sitikov A, Tang PM, Verin A and Zolinierowiecz S (1994) Serine/threonine protein phosphatases in the control of cell function. Adv Enzyme Regul 34:199-224.
6. Erdödi F, Csortos C, Bot G and Gergely P (1985) Separation of rabbit liver latent and spontaneously active phosphorylase phosphatase by chromatography on heparin-sepharose. Biochem Biophys Res Commun 128:705-712.
7. Garcia JGN, Davis HW and Patterson CE (1995) Regulation of endothelial cell gap formation and barrier dysfunction: Role of myosin light chain phosphorylation. J Cell Physiol 163:510-522.
8. Garcia JGN, Verin AD and Schaphorst KL (1996) Regulation of thrombin-mediated endothelial cell contraction and permeability. Semin Thromb Hemost 22:309-315.
9. Haeberle JR, Hathaway DR and DePaoli-Roach AA (1985) Dephosphorylation of myosin by the catalytic subunit of a type-2 phosphatase produces relaxation of chemically skinned uterine smooth muscle. J Biol Chem 260:9965-9968.
10. Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteege J and Stone SR (1988) The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2Aβ catalytic subunit. Nucleic Acids Res 16:11366.
11. Hempel A, Noll T, Muhs A and Piper HM (1996) Functional antagonism between cAMP and cGMP on permeability of coronary endothelial monolayers. Am J Physiol 270:H1264-H1271.
12. Herzig S, Meier A, Pfeiffer M and Neumann J (1995) Stimulation of protein phosphatases as a mechanism of the muscarinic receptor-mediated inhibition of cardiac L-type calcium channels. Pflugers Arch 429:531-538.
13. i and membrane currents in single bovine aortic endothelial cells. Am J Physiol 267:C1338-C1350.
14. Honkanen RE (1993) Cantharidin, another natural toxin that inhibits the activity of serine/threonine protein phosphatases types 1 and 2A. FEBS Lett 330:283-286.
15. Hunter T (1995) Protein kinases and phosphatases: The ying and yang of protein phosphorylation and signaling. Cell 80:225-236.
16. Knapp J, Bokník P, Huke S, Lüss H, Müller FU, Müller T, Nacke P, Schmitz W, Vahlensieck U and Neumann J (1998) The mechanism of action of cantharidin in smooth muscle. Br J Pharmacol 123:911-919.
17. Knapp J, Bokník P, Linck B, Lüss H, Müller FU, Nacke P, Neumann J, Vahlensieck U and Schmitz W (1997) The effect of the protein phosphatases inhibitor cantharidin on β-adrenoceptor-mediated vasorelaxation. Br J Pharmacol 120:421-428.
18. Kodama I, Kondo N and Shibata S (1986) Electromechanical effects of okadaic acid isolated from black sponge in guinea-pig ventricular muscles. J Physiol (Lond) 378:359-373.
19. Linck B, Bokník P, Knapp J, Müller FU, Neumann J, Schmitz W and Vahlensieck U (1996) Effects of cantharidin on force of contraction and phosphatase activity in nonfailing and failing human hearts. Br J Pharmacol 119:546-555.
20. Lum H and Malik AB (1994) Regulation of vascular endothelial barrier function. Am J Physiol 267:L223-L241.
21. Neumann J, Bokník P, Herzig S, Schmitz W, Scholz H, Gupta RC and Watanabe AM (1993) Evidence for physiological functions of protein phosphatases in the heart: Evaluation with okadaic acid. Am J Physiol 265:H257-266.
22. Neumann J, Eschenhagen T, Jones LR, Linck B, Schmitz W, Scholz H and Zimmermann N (1997) Increased expression of cardiac phosphatases in patients with end-stage heart failure. J Mol Cell Cardiol 29:265-272.
23. Neumann J, Herzig S, Bokník P, Apel M, Kaspareit G, Schmitz W, Scholz H, Tepel M and Zimmermann N (1995) On the cardiac contractile, biochemical and electrophysiological effects of cantharidin, a phosphatase inhibitor. J Pharmacol Exp Ther 274:530-539.
24. Noll T, Hempel A and Piper HM (1996) Neuropeptide Y reduces macromolecule permeability of coronary endothelial monolayers. Am J Physiol 271:H1878-H1883.
25. Sasaki K, Shima H, Kitagawa Y, Irino S, Sugimura T and Nagao M (1990) Identification of members of the protein phosphatase 1 gene family in the rat and enhanced expression of protein phosphatase 1a gene in rat hepatocellular carcinomas. Jpn J Cancer Res 81:1272-1280.
26. Shasby MD, Stevens T, Ries D, Moy AB, Kamath JM, Kamath AN and Shasby SS (1997) Thrombin inhibits myosin light chain dephosphorylation in endothelial cells. Am J Physiol 272:L311-L319.
27. Shenolikar S and Nairn AC (1991) Protein phosphatases: Recent progress, in Advances in Second Messenger and Phosphoprotein Research (Greengard P and Robinson GA eds) pp 1-121, Raven Press, Ltd., New York.
28. Shirazi A, Iizuka K, Fadden P, Mosse C, Somlyo AP, Somlyo AV and Haystead TAJ (1994) Purification and characterization of the mammalian myosin light chain phosphatase holoenzyme. J Biol Chem 269:31598-31606.
29. Siflinger-Birnboim A, Del Vecchio PJ, Cooper JA, Blumenstock FA, Shepard JM and Malik AB (1987) Molecular sieving characteristics of the cultured endothelial monolayer. J Cell Physiol 132:111-117.
30. Spahr R and Piper HM (1990) Microcarrier cultures of endothelial cells, in Cell Culture Techniques in Heart and Vessel Research (Piper HM ed) pp 220-229, Springer-Verlag, Berlin.
31. Stone SR, Mayer R, Wernet W, Maurer F, Hofsteenge J and Hemmings BA (1988) The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2Aα catalytic subunit. Nucleic Acids Res 16:11365.
32. Tachibana K, Sheuer PJ, Tsukitani Y, Kikuchi H, van Eugen E, Clardy J, Gopichand Y and Schmitz FJ (1981) Okadaic acid, a cytotoxic polyether from two marine sponges of the genus Halichondria. J Am Chem Soc 103:2469-2471.
33. Takai A, Bialojan C, Troschka M and Rüegg JC (1987) Smooth muscle myosin phosphatase inhibition and force enhancement by black sponge toxin. FEBS Lett 217:81-84.
34. Verin AD, Patterson CE, Day MA and Garcia JGN (1995) Regulation of endothelial cell gap formation and barrier function my myosin-associated phosphatase activities. Am J Physiol 269:L99-L108.
35. Wera S and Hemmings BA (1995) Serine/threonine protein phosphatases. Biochem J 311:17-29.