On-line sample clean-up and chromatography coupled with electrospray ionization mass spectrometry to characterize the primary sequence and disulfide bond content of recombinant calcium binding proteins

Biomedical Chromatography

Volumn 13, Issue 1, 1999, Pages 37-45

  Johnson, Kenneth L ^a   Veenstra, Timothy D ^a   Londowski, James M ^a   Tomlinson, Andy J ^a   Kumar, Rajiv ^a   Naylor, Stephen ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CALCIUM; CHROMATOGRAPHY; COVALENT BONDS; ELECTRODEPOSITION; ELECTROSPRAY IONIZATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; SULFUR COMPOUNDS;

BIOLOGICAL BUFFERS; CALBINDIN; CALCIUM BINDING PROTEINS; DELETION MUTANTS; DISULPHIDE BONDS; EF-HAND; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; PRIMARY SEQUENCES; RATS BRAIN; SAMPLE CLEAN-UP;

RECOMBINANT PROTEINS;

BUFFER; CALBINDIN; CALCIUM BINDING PROTEIN; CYSTEINE; MERCAPTOETHANOL; METHIONINE; PROTEINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; CHROMATOGRAPHY; COMPARATIVE STUDY; COVALENT BOND; DELETION MUTANT; DIMERIZATION; DISULFIDE BOND; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN EXPRESSION; PROTEIN ISOLATION; RAT;

EID: 0032989006     PISSN: 02693879     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-0801(199902)13:1<37::AID-BMC810>3.0.CO;2-P     Document Type: Article

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