A fluorescence energy transfer method for analyzing protein oligomeric structure: Application to phospholamban

Biophysical Journal

Volumn 76, Issue 5, 1999, Pages 2587-2599

  Li, Ming ^a   Reddy, Laxma G ^a   Bennett, Roberta ^a   Silva Jr , Norberto D ^b   Jones, Larry R ^c   Thomas, David D ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b MAYO CLINIC   (United States)

^c INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOLAMBAN;

ANIMAL CELL; ARTICLE; ENERGY TRANSFER; FLUORESCENCE SPECTROSCOPY; MATHEMATICAL ANALYSIS; NONHUMAN; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SARCOPLASMIC RETICULUM; SIMULATION;

EID: 0032988826     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77411-4     Document Type: Article

References (25)

1. Adair, B. D., and D. M. Engelman. 1994. Glycophorin a helical transmembrane domains dimerize in phospholipid bilayers: a resonance energy transfer study. Biochemistry. 33:5539-5544.
2. Arkin, I. T., P. D. Adams, K. R. MacKenzie, M. A. Lemmon, A. T. Brunger, and D. M. Engelman. 1994. Structure organization of the pentameric transmembrane α-helices of phospholamban, a cardiac ion channel. EMBO J. 13:4757-4764.
3. 2+-pump and phospholamban in Sf21 cells reveals new insights on ATPase regulation. J. Biol. Chem. 272:15872-15880.
4. Chen, R. F. 1966. Fluorescence quantum yield of 1-dimethyl-aminonaphthalene-5-sulphonate. Nature. 209:69.
5. Cornea, R. L., L. R. Jones, J. M. Autry, and D. D. Thomas. 1997. Mutation and phosphorylation change the oligomeric state of phospholamban in lipid bilayers. Biochemistry. 36:2960-2967.
6. Colyer, J. 1993. Control of the calcium pump of cardiac sarcoplasmic reticulum. A specific role for the pentameric structure of phospholamban? Cardiovasc. Res. 27:1766-1771.
7. Förster, T. 1948. Intermolecular energy migration and fluorescence. Ann. Phys. 2:55-75.
8. Kimura, Y., K. Kurzydlowski, M. Tada, and D. H. MacLennan. 1997. Phospholamban inhibitory function is activated by depolymerization. J. Biol. Chem. 272:15061-15064
9. 2+-selective channels in lipid bilayers. J. Biol. Chem. 263:18364-18368.
10. Li, M., R. L. Cornea, E. J. Olson, L. R. Jones, and D. D. Thomas. 1996. The oligomeric structure of phospholamban in membranes probed by fluorescence energy transfer. Biophys. J 70:A284.
11. Li, M., L. R. Jones, R. L. Cornea, and D. D. Thomas. 1998. Phosphorylation-induced structural change in phospholamban and its mutants, detected by intrinsic fluorescence. Biochemistry. 37:7869-7877.
12. 2+-ATPase activity in guinea ventricles. J. Biol. Chem. 258:464-471.
13. Luo, W., I. L. Grupp, J. Harrer, S. Ponniah, G. Grupp, J. J. Duffy, T. Doetschman, and E. G. Kranias. 1994. Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of β-agonist stimulation. Circ. Res. 75:401-409.
14. Moens, P. D., D. J. Yee, and C. G. dos Remedios. 1994. Determination of the radial coordinate of Cys-374 in F-actin using fluorescence resonance energy transfer spectroscopy: effect of phalloidin on polymer assembly. Biochemistry. 33:13102-13108.
15. Reddy, L. G., L. R. Jones, and D. D. Thomas. 1999. Depolymerization of phospholamban in the presence of calcium pump: a fluorescence energy transfer study. Biochemistry. In press.
16. 2+ ATPase. J. Biol. Chem. 270:9390-9397.
17. Schaffner, W., and C. Weissman. 1973. A rapid, sensitive, and specific method for the determination of protein in dilute solutions. Anal. Biochem. 56:502-514.
18. Schick, M. J. 1967. Nonionic Surfactants. Marcel Dekker, Inc., New York.
19. Simmerman, H. K. B., and L. R. Jones. 1998. Phospholamban: protein structure, mechanism of action, and role in cardiac function. Physiol. Rev. 78:921-947.
20. Simmerman, H. K. B., Y. M. Kobayashi, J. M. Autry, and L. R. Jones. 1996. A leucine zipper stabilizes the pentameric membrane domain of phospholamban and forms a coiled-coil pore structure. J. Biol. Chem. 271:5941-5946.
21. Vanderkooi, J. M., A. Lerokomar, H. Nakamura, and A. Martinosi. 1977. Fluorescence energy transfer between Ca transport ATPase molecules in artificial membranes. Biochemistry. 16:1262-1267.
22. Veatch, W., and L. Stryer. 1977. The dimeric nature of the gramicidin A transmembrane channel: conductance and fluorescence energy transfer studies of hybrid channels. J. Mol. Biol. 113:89-102
23. Watanabe, Y., Y. Kijima, M. Kadoma, M. Tada, and T. Takagi. 1991. Molar weight determination of phospholamban oligomer in the presence of sodium dodecyl sulfate: application of low-angle laser light scattering photometry. J. Biochem. 110:40-45.
24. Wegener, A. D., and L. R. Jones. 1984. Phosphorylation-induced mobility shift in phospholamban in sodium dodecyl sulfate-polyacrylamide gels. J. Biol. Chem. 259:1834-1841.
25. Wegener, A. D., H. K. B. Simmerman, J. P. Lindemann, and L. R. Jones. 1989. Phospholamban phosphorylation in intact ventricles. J. Biol. Chem. 264:11468-11474.