Method of melanin bleaching in MIB1-Ki67 immunostaining of pigmented lesions: A quantitative evaluation in malignant melanomas

Histochemical Journal

Volumn 31, Issue 4, 1999, Pages 237-240

  Li, Ling Xi Lawrence ^a,b   Crotty, Kerry A ^a,b   Kril, Jillian J ^b,c   Palmer, Allan A ^b   McCarthy, Stanley W ^b  

^a UNIVERSITY OF SYDNEY   (Australia)

^b ROYAL PRINCE ALFRED HOSPITAL   (Australia)

^c ANZAC Research Institute   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEN PEROXIDE; KI 67 ANTIGEN; MELANIN;

ANTIBODY LABELING; ARTICLE; BLEACHING; CELL COUNT; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MELANOMA; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CELL COUNT; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; HYDROGEN PEROXIDE; IMMUNOHISTOCHEMISTRY; KI-67 ANTIGEN; MELANINS; MELANOCYTES; MELANOMA; OXIDANTS; SKIN NEOPLASMS;

EID: 0032988798     PISSN: 00182214     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1003528507828     Document Type: Article

References (14)

1. Albrecht S, Jambrosic JA, Kahn HJ (1989) Nucleolar organizer regions in superficial spreading melanoma with nodule. Mod Pathol 2: 666-670.
2. Alexander RA, Hiscott PS, Hart RL, Grierson I (1986) Effect of melanin bleaching on immunoperoxidase, with reference to ocular tissues and lesions. Med Lab Sci 43: 121-127.
3. Cattoretti G, Becker MHG, Key G, Duchrow M, Schlüter C, Galle J, Gerdes J (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB1 and MIB3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 168: 357-363.
4. Gerdes J, Becker MHC, Key G, Cattoretti G (1992) Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 168: 85-87.
5. Gerdes J, Lemke H, Baisch H, Wacker H, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133: 1710-1715.
6. Gerdes J, Schwab U, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31: 13-20.
7. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291-302.
8. Hsu S-M, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabeled antibody (PAP) procedure. J Histochem Cytochem 29: 577-580.
9. Key G, Becker MHG, Baron B, Duchrow M, Schlüter C, Flad H-D, Gerdes J (1993) New Ki-67-equivalent murine monoclonal antibodies (MIB1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three base pair repetitive elements encoding for the Ki-67 epitope. Lab Invest 68: 629-636.
10. Mackie RM, White SI, Seywright MM, Young H (1989) An assessment of the value of AgNOR staining in the identification of dysplastic and other borderline melanocytic naevi. Br J Dermatol 120: 511-516.
11. McCormick D, Chong H, Hobbs C, Datta C, Hall PA (1993) Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1. Histopathology 22: 355-360.
12. Sawhney N, Hall PA (1992) Ki67-structure, function, and new antibodies. J Pathol 168: 161-162.
13. Talve LAI, Collan YUI, Alanen KA (1995) Bleaching of melanin before image cytometry of the DNA Content of pigmented skin tumours. Anal Quant Cytol Histol 17: 344-350.
14. Yu CC-W, Woods AL, Levison DA (1992) The assessment of cellular proliferation by immunohistochemistry: a view of currently available methods and their applications. Histochem J 24: 121-131.