Characterization of the exopolygalacturonate lyase PelX of Erwinia chrysanthemi 3937

Journal of Bacteriology

Volumn 181, Issue 5, 1999, Pages 1652-1663

  Shevchik, Vladimir E ^a   Kester, Harry C M ^b   Benen, Jacques A E ^b   Visser, Jaap ^b   Robert Baudouy, Janine ^a   Hugouvieux Cotte Pattat, Nicole ^a  

^a CNRS   (France)

^b Sect Molec Genet Indust M   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; DNA FRAGMENT; PECTATE LYASE; PECTIN;

AMINO ACID SEQUENCE; ARTICLE; CATABOLISM; ENTEROBACTERIACEAE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; ERWINIA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ERWINIA CHRYSANTHEMI;

EID: 0032988077     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.5.1652-1663.1999     Document Type: Article

References (46)

1. Alfano, J. R, J. H. Ham, and A. Collmer. 1995. Use of Tn5tacl toeluncapc/ gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysantherm EC16 mutant with deletions affecting the major pectate lyase isozymes. J. Bacteriol. 177:4553-4556.
2. Bardonnet, N., and C. Blanco. 1992. uidA antibiotic resistance cassettes for insertion mutagenesis, gene fusion and genetic constructions. FEMS Microbiol. Lett. 93:243-248.
3. Barras, F., F. Van Gijsegem, and A. K. Chatterjee. 1994. Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu. Rev. Phytopathol. 32:201-234.
4. Beaulieu, C., M. Boccara, and F. Van Gijsegem. 1993. Pathogenic behaviour of pectinase-defective Erwinia chrysanthemi mutants on different plants. Mol. Plant-Microbe Interact. 6:197-202.
5. Beer, S. Personal communication.
6. Benen, J., H. Kester, L. Parenicova, and J. Visser. 1996. Kinetics and mode of action of Aspergillus niger polygalacturonases. Prog. Biotechnol. 14:221-230.
7. Brooks, A. D., S. Y. He, S. Gold, N. T. Keen, A. Collmer, and S. W. Hutcheson. 1990. Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product. J. Bacteriol. 172:6950-6958.
8. Collmer, A., and D. F. Bateman. 1981. Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligogalacturonide lyase. Proc. Natl. Acad. Sci. USA 78:3920-3924.
9. Collmer, A., C. H. Whalen, S. V. Beer, and D. F. Bateman. 1982. An exopoly-alpha-D-galacturonosidase implicated in the regulation of extracellular pectate lyase production in Erwinia chrysanthemi. J. Bacteriol. 149:626-634.
10. Copeland, B. R., R. J. Richter, and C. E. Furlong. 1982. Renaturation and identification of periplasmic proteins in two-dimensional gels of Escherichia coli. J. Biol. Chem. 257:15065-15071.
11. Expert, D., and A. Toussaint. 1985. Bacteriocin-resistant mutants of Erwinia chrysanthemi: possible involvement of iron acquisition in phytopathogenicity. J. Bacteriol. 163:221-227.
12. Gilkes, N., B. Henrissat, D. Kilburn, J. R. Miller, and R. Warren. 1991. Domains in microbial β-1-4-glycanases: sequence conservation, function, and enzyme families. Microbiol. Rev. 55:303-315.
13. He, S. Y., and A. Collmer. 1990. Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16. J. Bacteriol. 172:4988-4995.
14. He, S. Y., M. Lindeberg, A. K. Chatterjee, and A. Collmer. 1991. Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. Proc. Natl. Acad. Sci. USA 88:1079-1083.
15. Heffron, S., B. Henrissat, M. D. Yoder, S. Lietzke, and F. Jurnak. 1995. Structure-based multiple alignment of extracellular pectate lyase sequences. Mol. Plant-Microbe Interact. 8:331-334.
16. Henrissat, B, S. E. Heffron, M. D. Yoder, S. E. Lietzke, and F. Jurnak. 1995. Functional implications of structure-based sequence alignment of proteins in the extracellular pectate lyase superfamily. Plant Physiol. 107:963-976.
17. Hugouvieux-Cotte-Pattat, N., G. Condemine, W. Nasser, and S. Reverchon. 1996. Regulation of pectinolysis in Erwinia chrysanthemi. Annu. Rev. Microbiol. 50:213-257.
18. Hugouvieux-Cotte-Pattat, N., H. Dominguez, and J. Robert-Baudouy, 1992. Environmental conditions affect the transcription of the pectinase genes of Erwinia chrysanthemi 3937. J. Bacteriol. 174:7807-7818.
19. Hugouvieux-Cotte-Pattat, N., S. Reverchon, and J. Robert-Baudouy. 1989. Expanded linkage map of Erwinia chrysanthemi strain 3937. Mol. Microbiol. 3:573-581.
20. Johnson, B. H., and M. H. Hecht. 1994. Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and !hawing. Biol Technology 12:1357-1360.
21. Keen, N. T., D. Dahlbeck, B. Staskawicz, and W. Belser. 1984. Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expres-sion in Escherichia coli. J. Bacteriol. 159:825-831.
22. Kester, H., and J. Visser. 1990. Purification and characterization of polygalacturonases produced by the hyphal fungus Aspergillus niger. Biotechnol. Appl. Biochcm. 12:150-160.
23. Lojkowska, E., C. Dorel, P. Reignault, N. Hugouvieux-Cotte-Pattat, and J. Robert-Baudouy. 1993. Use of GUS fusions to study the expression of Erwinia chrysanthemi pectinase genes during infection of potato tubers. Mol. Plant-Microbe Interact. 6:488-494.
24. Lojkowska, E., C. Masclaux, M. Boccara, J. Robert-Baudouy, and N. Hugouvieux-Cotte-Pattat. 1995. Characterization of the pell. gene encoding a novel pectate lyase of Erwiniaia chrysanthemi 3937. Mol. Microbiol. 16:1183-1195.
25. Mengin-Lecreulx, D., and J. van Heijenoort. 1993. Identification of they glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli. J. Bacteriol 175:6150-6157.
26. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
27. Moran, F., S. Nasuno, and M. P. Starr. 1968. Extracellular and intracellular polygalacturonic acid trans eliminase of Erwinia carotovora. Arch. Biochem. Biophys. 123:298-306.
28. Moran, F., S. Nasuno, and M. P. Starr. 1968. Oligogalacturonide transeliminase of Enwinia carotovora. Arch. Biochem. Biophys. 125:734-741.
29. Nasser, W., S. Reverchon, G. Condemine, and J. Robert-Baudouy. 1994. Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis. J. Mol. Biol. 236:427-440.
30. Pissavin, C., J. Robert-Baudouy, and N. Hugouvieux-Cotte-Pattat. 1996. REgulation of pelZ. a gene of the pelBC cluster encoding a new pectate lyase in Erwinia chrysanthemi 3937. J. Bacteriol. 178:7187-7196.
31. Preston, J., J. Rice, L. Ingram, and N. T. Keen. 1992. Differential depolymerization mechanisms of pectate lyase secreted by Erwinia chrysanthemi EC16. J. Bacteriol. 174:2039-2042.
32. Resibois, A., M. Colet, M. Faelen, E. Schoonejans, and A. Toussaint. 1984. PhiEC2, a new generalised transducing phage of Erwinia chrysanthemi. Virology 137:102-112.
33. Reverchon, S., D. Expert, J. Robert-Baudouy, and W. Nasser. 1997. The cyclic AMP receptor protein is the main activator of the pectinolysis genes in Erwinia chrysanthemi. J. Bacteriol. 179:3500-3508.
34. Reverchon, S., W. Nasser, and J. Robert-Baudouy. 1991. Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation. Mol. Microbiol. 5:2203-2216.
35. Reverchon, S., W. Nasser, and J. Robert-Baudouy. 1994. pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi. Mol. Microbiol. 11:1127-1139.
36. Ried, J. L., and A. Collmer. 1986. Comparison of pectic enzymes produced by Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, and Erwinia carotovora subsp. atroseptica. Appl. Environ. Microbiol. 52:305-310.
37. Roeder, D. L., and A. Collmer. 1985. Marker-exchange mutagenesis of pectate lyase isozyme gene in Erwinia chrysanthemi. J. Bacteriol. 164:51-56.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
39. Saraste, M., N. Gay, M. Runswik, and J. Walker. 1981. The atp operon: nucleotide sequence of the genes for the gamma, beta, and epsilon subunits of Escherichia coli ATP synthase. Nucleic Acids Res. 9:5287-5296.
40. Shevchik, V. E., J. Robert-Baudony, and N. Hugouvieux-Cotte-Pattat. 1997. The pectate lyase Pell of Erwinia chrysanthemi belongs to a new family. J. Bacteriol. 179:7321-7330.
41. Studier. W. F., and B. A. Moffat. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
42. Surgey, N., J. Robert-Baudouy, and G. Condemine. 1996. The Erwinia chrysanthemi pecT gene regulates pectinase gene expression. J. Bacteriol. 178: 1593-1599.
43. Tabor, S., and C. Richardson. 1985. A bacteriophage T7 RNA polymerase. promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
44. Tardy, F., W. Nasser, J. Robert-Baudouy, and N. Hugouvieux-Cotte-Pattat. 1997. Comparative analysis of the five major Erwinia chrysantheni pectate lyases: enzyme characteristics and potential inhibitors. J. Bacteriol. 179: 2503-2511.
45. Van Gijsegem, F., A. Toussaint, and E. Schoonejans. 1985. In vivo cloning of pectate lyase and cellulase genes of Erwinia chrysanthemi. EMBO J. 4:787-795.
46. Wandersman, C., M. Schwartz, and T. Ferenci. 1979. Escherichia coli mutants impaired in maltodextrin transport. J. Bacteriol. 140:1-13.