Chemiluminescent detection of sequential DNA hybridizations to high- density, filter-arrayed cDNA libraries: A subtraction method for novel gene discovery

BioTechniques

Volumn 27, Issue 1, 1999, Pages 146-152

  Guiliano, D ^a   Ganatra, M ^b   Ware, J ^b   Parrot, J ^b   Daub, J ^a   Moran, L ^b   Brennecke, H ^b   Foster, J M ^b   Supali, T ^c   Blaxter, M ^a   Scott, A L ^d   Williams, S A ^e   Slatko, B E ^b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b NEW ENGLAND BIOLABS   (United States)

^c University of Jakarta   (Indonesia)

^d JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^e SMITH COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ARTICLE; BRUGIA MALAYI; CHEMOLUMINESCENCE; DNA DETERMINATION; DNA HYBRIDIZATION; DNA LIBRARY; DNA PROBE; DNA SEQUENCE; GENETIC ANALYSIS; NONHUMAN;

BACTERIA (MICROORGANISMS); BRUGIA MALAYI;

EID: 0032987748     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99271rr03     Document Type: Article

References (19)

1. Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1989. Current Protocols in Molecular Biology. Wiley-Interscience, New York.
3. Birren, B., E.D. Green, S. Klapholz, R. Myers and J. Roslams. 1997. Genome Analysis: A Laboratory Manual. CSH Laboratory Press. Cold Spring Harbor, NY.
4. Blaxter, M.L. 1995. The Filarial Genome Project. Parasitol. Today 11:811-812.
5. Blaxter, M.L. and M. Aslett 1997. Internet resources for the parasite genome projects. Trends Genet. 13:40-41.
6. Blaxter, M.L., J. Daub, M. Waterfall, D. Guiliano, S. Williams, K. Jayaraman, R. Ramzy, B. Slatko and A.L. Scott 1998. The Filarial Genome Project. In COST 819. The Commission of the European Community, Brussels. p. 47-58.
7. Blaxter, M.L., N. Raghavan, I. Ghosh, D. Guiliano, W. Lu, S.A. Williams, B. Slatko and A.L. Scott. 1996. Genes expressed in Brugia malayi infective third stage larvae. Mol. Biochem. Parasitol. 77:77-93.
8. Creasey, A., L. D'Angio, T.S. Dunne, C. Kissinger, T. O'Keefe, H. Perry-O'Keefe, L. Moran, M. Roskey, I. Schildkraut, L.E. Sears and B. Slatko. 1991. Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing. BioTechniques 11:102-109.
9. Dubltsky, A., J. Brown and H. Brandwein. 1992. Chemiluminescent detection of DNA on nylon membranes. BioTechniques 13:392-400.
10. Kern, S. and G.M. Hampton. 1997. Direct hybridization of large-insert genomic clones on high-density gridded filter arrays. BioTechniques 23:120-124.
11. Kincaid, R.L. and M.S. Nightingale. 1988. A rapid non-radioactive procedure for plaque hybridization using biotinylated probes prepared by random primed labeling. BioTechniques 6:42-49.
12. New England Biolabs. 1997. Phototope®-Star Detection Manual. Beverly, MA.
13. Ottesen, E.A. and C.D. Ramachandran. 1995. Lymphatic filariasis. Infection and disease: control strategies. Parasitol. Today 11:129-131.
14. Pomrehn, A. 1997. The construction of a Brugia malayi cosmid library for analysis of B. malayi genome organization. Northhampton, MA: Smith College; Thesis.
15. Pope-Chappell, J. 1997. Construction and characterization of a genomic Brugia malayi library using bacterial artificial chromosomes. Northhampton, MA: Smith College; Thesis.
16. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
17. Sheffield, J.S., W.H. Benjamin, Jr. and L.S. McDaniel. 1992. Detection of DNA in Southern blots by chemiluminescence is a sensitive and rapid technique. BioTechniques 12:836-839.
18. Unasch, T.R. 1994. The filarial genome project. Parasitol. Today 10:415-416.
19. Weintraub, D., P. Ulanch and J. Mullet 1998. Screening BAC high density filters with Southern-Star™. Luminescence Topics 6:3-4.