Cloning of the transketolase gene and the effect of its dosage on aromatic amino acid production in Corynebacterium glutamicum

Applied Microbiology and Biotechnology

Volumn 51, Issue 2, 1999, Pages 201-206

  Ikeda, M ^a   Okamoto, K ^a   Katsumata, R ^b  

^a KYOWA HAKKO KOGYO CO LTD   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC AMINO ACID; GLUCONIC ACID; LYSINE; PHENYLALANINE; RIBOSE; SHIKIMIC ACID; TRANSKETOLASE; TRYPTOPHAN;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE DOSAGE; GENE OVEREXPRESSION; GENETIC COMPLEMENTATION; GLYCOLYSIS; MOLECULAR CLONING; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PLASMID; PLEIOTROPY;

AMINO ACIDS; BLOTTING, SOUTHERN; CLONING, MOLECULAR; CORYNEBACTERIUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE DOSAGE; GENES, BACTERIAL; RESTRICTION MAPPING; TRANSKETOLASE;

EID: 0032986593     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051382     Document Type: Article

References (33)

1. Backman K, Ptashne M, Gilbert W (1976) Construction of plasmids carrying the cI gene of bacteriophage λ. Proc Natl Acad Sci USA 73: 4174-4178
2. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
3. 2 fixation operon of Rhodobacter sphaeroides. J Biol Chem 266: 20447-20452
4. Draths KM, Frost JW (1990) Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J Am Chem Soc 112: 1657-1659
5. Eidels L, Osborn MJ (1971) Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium. Proc Natl Acad Sci USA 68: 1673-1677
6. Fletcher TS, Kwee IL, Nakada T, Largman C, Martin BM (1992) DNA sequence of the yeast transketolase gene. Biochemistry 31: 1892-1896
7. Gynheung A, Friesen JD (1979) Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol 140: 400-407
8. Himmo SD, Thomson M, Gubler CJ (1988) Isolation of transketolase from human red blood cells. Prep Biochem 18: 261-276
9. Iida A, Teshiba S, Mizobuchi K (1993) Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12. J Bacteriol 175: 5375-5383
10. Ikeda M, Katsumata R (1992) Metabolic engineering to produce tyrosine or phenylalanine in a tryptophan-producing Corynebacterium glutamicum strain. Appl Environ Microbiol 58: 781-785
11. Ikeda M, Katsumata R (1994) Transport of aromatic amino acids and its influence on overproduction of the amino acids in Corynebacterium glutamicum. J Ferment Bioeng 78: 420-425
12. Ikeda M, Katsumata R (1998) A novel system with positive selection for the chromosomal integration of replicative plasmid DNA in Corynebacterium glutamicum. Microbiology 144: 1863-1868
13. Ikeda M, Ozaki A, Katsumata R (1993) Phenylalanine production by metabolically engineered Corynebacterium glutamicum with the pheA gene of Escherichia coli. Appl Microbiol Biotechnol 39: 318-323
14. Ikeda M, Okamoto K, Katsumata R (1998) A transketolase mutant of Corynebacterium glutamicum. Appl Microbiol Biotechnol 50: 375-378
15. Katsumata R, Ikeda M (1993) Hyperproduction of tryptophan in Corynebacterium glutamicum by pathway engineering. Biotechnology 11: 921-925
16. Katsumata R, Ozaki A, Oka T, Furuya A (1984) Protoplast transformation of glutamate-producing bacteria with plasmid DNA. J Bacteriol 159: 306-311
17. Kinoshita S, Nakayama K (1987) Amino acids. In: Rose AH (ed) Primary products of metabolism. Academic Press, London New York San Francisco, pp 209-261
18. Kochetov GA (1982) Transketolase from yeast, rat liver and pig liver. Methods Enzymol 90: 209-223
19. Koplow J, Goldfine H (1974) Alterations in the outer membrane of the cell envelop of heptose-deficient mutants of Escherichia coli. J Bacteriol 117: 527-543
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
21. Masri SW, Ali M, Gubler CJ (1988) Isolation of transketolase from rabbit liver and comparison of some of its kinetic properties with transketolase from other sources. Comp Biochem Physiol [B] 90: 167-172
22. Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101: 20-78
23. Metzger MH, Hollenberg CP (1994) Isolation and characterization of the Pichia stipitis transketolase gene and expresstion in a xylose-utilising Saccharomyces cerevisiae transformant. Appl Microbiol Biotechnol 42: 319-325
24. Mocali A, Paoletti F (1989) Transketolase from human leukocytes. Isolation, properties and induction of polyclonal antibodies. Eur J Biochem 180: 213-219
25. Ozaki A, Katsumata R, Oka T, Furuya A (1984) Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum. Mol Gen Genet 196: 175-178
26. Ozaki A, Katsumata R, Oka T, Furuya A (1985) Cloning of the genes concerned in phenylalanine biosynthesis in Corynebacterium glutamicum and its application to breeding of a phenylalanine producing strain. Agric Biol Chem 49: 2925-2930
27. Saito H, Miura K (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72: 619-629
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
29. Sasajima K, Yoneda M (1984) Production of pentoses by microorganisms. Biotechnol Genet Eng Rev 2: 175-213
30. Schaaff-Gerstenschlager I, Mannhaupt G, Vetter I, Zimmermann FK, Feldmann H (1993) TKL2, a second transketolase gene of Saccharomyces cerevisiae: cloning, sequence and deletion analysis of the gene. Eur J Biochem 217: 487-492
31. Sprenger GA (1993) Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene. Biochim Biophys Acta 1216: 307-310
32. Sundström M, Lindqvist Y, Schneider G, Hellman U, Ronne H (1993) Yeast TKL1 gene encodes a transketolase that is required for efficient glycolysis and biosynthesis of aromatic amino acids. J Biol Chem 268: 24346-24352
33. Takeuchi T, Nishino K, Itokawa Y (1986) Purification and characterization of, and preparation of an antibody to, transketolase from human red blood cells. Biochim Biophys Acta 872: 24-32