Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322

Journal of Bacteriology

Volumn 181, Issue 5, 1999, Pages 1689-1693

  Jewell, Jean E ^a   Orwick, Jill ^a   Liu, Jun ^a   Miller, Kurt W ^a  

^a UNIVERSITY OF WYOMING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL PROTEIN; CYSTEINE; DNA; TETRACYCLINE;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; CODON; ENTEROBACTERIACEAE; GENOTYPE; MINIMUM INHIBITORY CONCENTRATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0032985477     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.5.1689-1693.1999     Document Type: Article

References (36)

1. Allard, J. D., and K. P. Bertrand. 1992. Membrane topology of the pBR322 tetracycline resistance protein. J. Biol. Chem. 267:17809-17819.
2. Allard, J. D., and K. P. Bertrand. 1993. Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins. J. Bacteriol. 175:4554-4560.
3. Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heynecker, H. W. Boyer, J. H. Crosa, and S. Falkow. 1977. Construction and characterization of a new cloning vehicle. II. A multipurpose cloning system. Gene 2:95-113.
4. Bulaj, G., T. Kortemme, and D. P. Goldenberg. 1998. Ionization-reactivity relationships for cysteine thiols in polypeptides. Biochemistry 37:8965-8972.
5. Duplay, P., H. Bedouelle, A. Fowler, I. Zabin, W. Saurin, and M. Hofnung. 1984. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K-12. J. Biol. Chem. 259:10606-10613.
6. Eckert, B., and C. F. Beck. 1989. Topology of the transposon Tn10-encoded tetracycline resistance protein within the inner membrane of Escherichia coli. J. Biol. Chem. 264:11663-11670.
7. Glavas, N. A., C. Hou, and P. D. Bragg. 1995. Organization in the membrane of the N-terminal proton-translocating domain of the β subunit of the pyridine nucleotide transhydrogenase of Escherichia coli. Biochem. Biophys. Res. Commun. 214:230-238.
8. Gregory, J. D. 1955. The stability of N-ethylmaleimide and its reaction with sulfhydryl groups. J. Am. Chem. Soc. 77:3922-3923.
9. Guo, D., J. Liu, A. Motlagh, J. Jewell, and K. W. Miller. 1996. Efficient insertion of odd-numbered transmembrane segments of the tetracycline resistance protein requires even-numbered segments. J. Biol. Chem. 271:30829-30834.
10. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
11. + antiporter. Biochemistry 35:15896-15899.
12. + antiporter of Escherichia coli is essential for substrate binding. FEBS Lett. 388:50-52.
13. + antiporter as studied by site-directed chemical labeling. J. Biol. Chem. 272:580-585.
14. + antiporter. J. Biol. Chem. 273:5243-5247.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
16. Levy, S. B. 1984. Resistance to the tetracyclines, p. 191-240. In L. E. Bryan (ed.), Antimicrobial drug resistance. Academic Press, New York, N.Y.
17. Liu, T.-Y. 1977. The role of sulfur in proteins, p. 239-402. In H. Neurath, R. L. Hill, and C.-L. Boeder (ed.), The proteins. Academic Press, New York, N.Y.
18. Loo, T. W., and D. M. Clarke. 1995. Membrane topology of a cysteine-less mutant of human P-glycoprotein. J. Biol. Chem. 270:843-848.
19. Matos, M., M.-C. Fann, R.-T. Yan, and P. C. Maloney. 1996. Enzymatic and biochemical probes of residues external to the translocation pathway of UhpT, the sugar phosphate carrier of Escherichia coli. J. Biol. Chem. 271: 18571-18575.
20. McMurry, L. M., M. Stephan, and S. B. Levy. 1992. Decreased function of the class B tetracycline efflux protein Tet with mutations at aspartate 15, a putative intramembrane residue. J. Bacteriol. 174:6294-6297.
21. McNicholas, P., I. Chopra, and D. M. Rothstein. 1992. Genetic analysis of the tetA(C) gene on plasmid pBR322. J. Bacteriol. 174:7926-7933.
22. Miller, K. W., P. L. Konen, J. Olson, and K. M. Ratanavanich. 1993. Membrane protein topology determination by protcolysis of maltose binding protein fusions. Anal. Biochem. 215:118-128.
23. Miller, K. W., and J. E. Jewell. 1995. Identification of a topology control domain in the tetracycline resistance protein. Arch. Biochem. Biophys. 322: 445-452.
24. Nguyen, T. T., K. Postle, and K. P. Bertrand. 1983. Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322. Gene 25:83-92.
25. Nikaido, H., and M. H. Saier, Jr. 1992. Transport proteins in bacteria: common themes in their design. Science 258:936-942.
26. Rubin, R. A., S. B. Levy, R. L. Heinrikson, and F. J. Kezdy. 1990. Gene duplication in the evolution of the two complementing domains of gram-negative bacterial tetracycline efflux proteins. Gene 87:7-13.
27. Rubin, R. A., and S. B. Levy. 1991. Tet protein domains interact productively to mediate tetracycline resistance when present on separate polypeptides. J. Bacteriol. 173:4503-4509.
28. Taylor, J. W., J. Ott, and F. Eckstein. 1985. The rapid generation of oligo-nucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res. 13:8765-8785.
29. Yamaguchi, A., T. Udagawa, and T. Sawai. 1990. Transport of divalent cations with tetracycline as mediated by the transposon Tn10-encoded tetracycline resistance protein. J. Biol. Chem. 265:4809-4813.
30. 66, in tetracycline transport. J. Biol. Chem. 265:15525-15530.
31. + translocation. J. Biol. Chem. 266:6045-6051.
32. + antiporter of Escherichia coli. FEBS Lett. 307:229-232.
33. + antiporter of Escherichia coli encoded by transposon Tn10. Roles of aspartyl residues located in the putative transmembrane helices. J. Biol. Chem. 267:7490-7498.
34. + antiporter of Escherichia coli encoded by transposon Tn10. The role of a conserved sequence motif, GXXXXRXGRR, in a putative cytoplasmic loop between helices 2 and 3. J. Biol. Chem. 267:19155-19162.
35. + antiporter of Escherichia coli. Biochemistry 32:5698-5704.
36. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.