Heterologous expression of soluble methane monooxygenase genes in methanotrophs containing only particulate methane monooxygenase

Archives of Microbiology

Volumn 171, Issue 6, 1999, Pages 364-370

  Lloyd, John S ^a,b   De Marco, Paolo ^a,c   Dalton, Howard ^a   Murrell, J Colin ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b CYTOCELL LTD   (United Kingdom)

^c UNIVERSITY OF PORTO   (Portugal)

Author keywords

Heterologous expression; Methane monooxygenase; Methane oxidation; Methanotroph; Regulation

Indexed keywords

METHANE MONOOXYGENASE; PLASMID VECTOR;

ARTICLE; BACTERIAL GROWTH; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE EXPRESSION REGULATION; GENETIC VARIABILITY; METHANOTROPHIC BACTERIUM; METHYLOBACTERIUM; METHYLOCOCCACEAE; MOLECULAR GENETICS; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS;

CLONING, MOLECULAR; CONJUGATION, GENETIC; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; METHYLOCOCCACEAE; OXYGENASES; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); METHYLOBACTERIUM; METHYLOCOCCACEAE; METHYLOCOCCUS CAPSULATUS; METHYLOCYSTIS PARVUS; METHYLOMICROBIUM ALBUM; METHYLOSINUS TRICHOSPORIUM; TRICHOSPORIUM; UNIDENTIFIED BACTERIOPHAGE;

EID: 0032980205     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002030050723     Document Type: Article

References (40)

1. Bodrossy L, Murrell JC, Dalton H, Kalman M, Puskas LG, Kovacs KL (1995) Heat-tolerant methanotrophic bacteria from the hot water effluent of a natural gas field. Appl Environ Microbiol 61:3549-3555
2. Brusseau GA, Tsien H-C, Hanson RS, Wackett LP (1990) Optimisation of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity. Biodegradation 1:19-29
3. Cardy DLN, Laidler V, Salmond GPC, Murrell JC (1991a) Molecular analysis of the methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b. Mol Microbiol 5:335-342
4. Cardy DLN, Laidler V, Salmond GPC, Murrell JC (1991b) The methane monooxygenase (MMO) gene cluster of Methylosinus trichosporium OB3b: cloning and sequencing of the mmoC gene. Arch Microbiol 156:477-483
5. - cloning vectors. 1. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene 68:139-149
6. Colby J, Dalton H (1978) Resolution of the methane monooxygenase of Methylococcus capsulatus (Bath) into three components. Biochem J 171:461-468
7. 1 Compounds, 27 August-1 September 1995, San Diego, CA, USA
8. Dalton H, Whittenbury R (1976) The acetylene reduction technique as an assay for the nitrogenase activity m the methane-oxidising bacterium Methylococcus capsulatus Bath. Arch Microbiol 109:147-151
9. 1 compounds. Intercept Press, Andover, UK, pp 65-80
10. Elango N, et al (1997) Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b. Protein Sci 6:556-568
11. Fox BG, Froland WA, Dege JE, Lipscomb JD (1989) Methane monooxygenase from Methylosinus trichosporium OB3b. Purification and properties of a three component system with high specific activity from a type II methanotroph. J Biol Chem 264:10023-10033
12. Green J, Dalton H (1985) Protein B of the soluble methane monooxygenase from Methylococcus capsulatus (Bath): a novel regulatory protein of enzyme activity. J Biol Chem 260: 15795-15801
13. Hanson R S, Hanson TE (1996) Methanotrophic bacteria. Microbiol Rev 60:439-471
14. Jahng D, Wood TK (1994) Trichloroethylene and chloroform degradation by a recombinant Pseudomonad expressing soluble methane monooxygenase from Methylosinus trichosporium OB3b. Appl Environ Microbiol 60:2473-2482
15. Knauf VC, Nester EW (1982) Wide host range cloning vectors: a cosmid clone bank of Agrobacterium Ti plasmids. Plasmid 8:45-54
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680-685
17. Lidstrom ME, Wopat AE (1984) Plasmids in methanotrophic bacteria: isolation, characterisation and DNA hybridisation analysis. Arch Microbiol 140:27-33
18. Lipscomb JD (1994) Biochemistry of the soluble methane monooxygenase. Annu Rev Microbiol 48:371-399
19. Lloyd JS, Bhambra A, Murrell JC, Dalton H (1997) Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gln modification. Eur J Biochem 248:7 2-79
20. Lloyd JS, Finch R, Dalton H, Murrell JC (1999) Homologous expression of soluble methane monooxygnease genes in Methylosinus trichosporium OB3b. Microbiol UK 145:461-470
21. - recipient strains of Escherichia coli K12. Proc Natl Acad Sci 60:160-167
22. Lund J, Woodland MP, Dalton H (1985) Electron transfer reactions in the soluble methane monooxygenase of Methylococcus capsulatus (Bath). Eur J Biochem 147:297-305
23. Martin H (1994) Molecular genetics of methane oxidation in Methylosinus trichosporium OB3b. PhD Thesis, University of Warwick, UK
24. Murrell JC (1994) Molecular genetics of methane oxidation. Biodegradation 5:145-159
25. Nguyen HHT, Elliott SJ, Yip JHK, Chan SI (1998) The particulate methane monooxygenase from Methylococcus capsulatus (Bath) is a novel copper-containing three-subunit enzyme. Isolation and characterisation. J Biol Chem 273:7957-7966
26. Nielsen AK, Gerdes K, Degn H, Murrell JC (1996) Regulation of bacterial methane oxidation: transcription of the soluble methane monooxygenase operon of Methylococcus capsulatus (Bath) is repressed by copper ions. Microbiol UK 142:1289-1296
27. Nielsen AK, Gerdes K, Murrell JC (1997) Copper-dependent reciprocal transcriptional regulation of methane monooxygenase genes in Methylococcus capsulatus and Methylosinus trichosporium. Mol Microbiol 25:399-409
28. Pilkington SJ, Dalton H (1990) Soluble methane monooxygenase from Methylococcus capsulatus (Bath). Methods Enzymol 188:181-190
29. Rosenzweig AC, Frederick CA, Lippard SJ, Nordlund P (1993) Crystal structure of a bacterial non-haem iron hydroxylase that catalyses the biological oxidation of methane. Nature 366:537-543
30. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
31. Saunders SE, Burke JF (1990) Rapid isolation of miniprep DNA for double strand sequencing. Nucleic Acids Res 18:4948-4949
32. Semrau JD, et al (1995) Particulate methane monooxygenase genes in methanotrophs. J Bacteriol 177:3071-3079
33. Simon R, Priefer U, Pühler A (1983) A broad-host-range mobilisation system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1:784-791
34. Stainthorpe AC, Murrell JC, Salmond GPC, Dalton H, Lees V (1989) Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath). Arch Microbiol 152:154-159
35. Stainthorpe AC, Lees V, Salmond GPC, Dalton H, Murrell JC (1990) The methane monooxygenase gene cluster from Methylococcus capsulatus (Bath). Gene 91:27-34
36. Stanley SH, Prior SD, Leak DJ, Dalton H (1983) Copper stress underlies the fundamental change in intracellular location of methane monooxygenase in methane-oxidising organisms: studies in batch and continuous cultures. Biotechnol Lett 5: 487-492
37. Tsien H-C, Brusseau GA, Hanson RS, Wackett LP (1989) Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl Environ Microbiol 55:3155-3161
38. West CA, Salmond GPC, Dalton H, Murrell JC (1992) Functional expression m Escherichia coli of proteins B and C from soluble methane monooxygenase of Methylococcus capsulatus (Bath). J Gen Microbiol 138:1301-1307
39. Woodland MP, Dalton H (1989) Purification and characterisation of Component A of the methane monooxygenase from Methylococcus capsulatus (Bath). J Biol Chem 259:53-59
40. Zahn JA, DiSpirito AA (1996) Membrane associated methane monooxygenase from Methylococcus capsulatus (Bath). J Bacteriol 178:1018-1029