Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients

Journal of Virological Methods

Volumn 78, Issue 1-2, 1999, Pages 153-162

  Binder, Thomas ^a,c   Siegert, Wolfgang ^a   Kruse, Anja ^a   Oettle, Helmut ^a   Wilborn, Freimut ^a,d   Peng, Ruoqi ^a   Timm, Horst ^e   Neuhaus, Peter ^b   Schmidt, Christian A ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Klinik fur Allgemein Viszeral und Transplantationschirurgie   (Germany)

^c FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^d BioteCon Gesell B   (Germany)

^e ROBERT KOCH INSTITUTE   (Germany)

Author keywords

Glycoprotein B; Glycoprotein H; Human cytomegalovirus; Liver transplantation; PCR single strand polymorphism analysis; Restriction fragment length polymorphism; Strains variants

Indexed keywords

GLYCOPROTEIN; VIRUS ENVELOPE PROTEIN;

ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; DNA POLYMORPHISM; DNA SEQUENCE; HUMAN; HUMAN TISSUE; LIVER TRANSPLANTATION; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; VIRUS CHARACTERIZATION; VIRUS STRAIN;

BASE SEQUENCE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; HUMANS; LIVER TRANSPLANTATION; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; POSTOPERATIVE COMPLICATIONS; SEQUENCE ANALYSIS, DNA; VARIATION (GENETICS); VIRAL ENVELOPE PROTEINS;

HUMAN HERPESVIRUS 5;

EID: 0032978352     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(98)00173-6     Document Type: Article

References (25)

1. Binder T., Berg T., Siegert W., Schmidt CA. PCR-SSCP: non-radioisotopic detection with biotinylated primers and streptavidin-alkaline phosphatase conjugate. BioTechniques. 18:1995;780-781.
2. Britt W.J., Vugler L., Stephens E.B. Induction of complement-dependent and -independent neutralizing antibodies by recombinant-derived human cytomegalovirus gp55-116. J. Virol. 62:1988;3309-3318.
3. Cha T.-A., Tom E., Kemble E.T., Duke G.M., Mocarski E.S., Spaete R.R. Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J. Virol. 70:1996;78-83.
4. Chou S. Differention of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens. J. Infect. Dis. 162:1990;738-742.
5. Chou S. Comparative analysis of sequence variation in gp116 and gp55 components of glycoprotein B of human cytomegalovirus. Virology. 188:1992;388-390.
6. Chou S. Molecular epidemiology of envelope glycoprotein H of human cytomegalovirus. J. Infect. Dis. 166:1992;604-607.
7. Chou S., Dennison K.M. Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization related epitopes. J. Infect. Dis. 163:1991;1229-1234.
8. Drew L. Cytomegalovirus infections in patients with AIDS. J. Infect. Dis. 158:1988;449-456.
9. Fries B.C., Chou S., Boeckh M., Torok-Storb B. Frequency distribution of cytomegalovirus envelope glycoprotein genotypes in bone marrow transplantat recipients. J. Infect. Dis. 169:1994;769-774.
10. Hayashi K. PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA. PCR Methods Appl. 1:1991;34-38.
11. Jiwa N.M., Van Gemmert G.W., Raap A.K. et al. Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation. 48:1989;72-76.
12. Lehner R., Stamminger T., Mach M. Comparative sequence analysis of human cytomegalovirus strains. J. Clin. Microbiol. 29:1991;2494-2502.
13. Orita M., Iwahana H., Kanazawa H., Hayashi K., Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA. 86:1989;2766-2770.
14. Orita M., Suzuki Y., Sekiya T., Hayashi K. A rapid and sensitive detection of point mutations and genetic polymorphisms using polymerase chain reaction. Genomics. 5:1989;874-879.
15. Rasmussen L., Matkin C., Spaete R., Pachl C., Merigan T.C. Antibody response to human cytomegalovirus glycoproteins gB and gH after natural infections in humans. J. Infect. Dis. 164:1991;835-842.
16. Rasmussen L., Hong C., Zipeto D. et al. Cytomegalovirus gB distribution differs in human immunodeficiency virus-infected patients and immunocompromised allograft recipients. J. Infect. Dis. 175:1997;179-184.
17. Schmidt C.A., Oettle H., Neuhaus P., Wiens M., Timm H., Wilborn F.F., Siegert W. Demonstration of cytomegalovirus by polymerase chain reaction after liver transplantation. Transplantation. 56:1993;872-874.
18. Schmidt C.A., Oettle H., Jessen J. et al. Demonstration of cytomegalovirus after bone marrow transplantation by polymerase chain reaction, virus culture and antigen detection in buffy coat leukocytes. Bone Marrow Transplant. 13:1994;71-75.
19. Shepp D.H., Match M.E., Ashraf A.B., Lipson S.M., Millan C., Pergolizzi R. Cytomegalovirus glycoprotein B groups associated with retinitis in AIDS. J. Infect. Dis. 174:1996;184-187.
20. Simmons P., Kaushansky K., Torok-Storb B. Mechanisms of cytomegalovirus-mediated myelosuppression: perturbation of stromal cell function versus direct infection of myeloid cells. Proc. Natl. Acad. Sci. USA. 87:1990;1386-1390.
21. Spaete R.R., Thayr R.M., Probert W.S. et al. Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology. 167:1988;207-225.
22. Torok-Storb B., Boeckh M., Hoy C., Leisenring W., Myerson D., Gooley T. Association of specific cytomegalovirus genotypes with death from myelosuppression after marrow transplantation. Blood. 90:1997;2097-2102.
23. Torok-Storb B., Fries B., Stachel D., Khaira D. Cytomegalovirus: variations in tropism and disease. Leukemia. 7(Suppl 2):1993;S83-S85.
24. Urban M., Klein M., Britt W., Haßfurther E., Mach M. Glycoprotein H of human cytomegalovirus is a major antigen for the neutralizing humoral immune response. J. Gen. Virol. 77:1996;1537-1547.
25. Wilborn W., Binder T., Neipel F., Blasczyk R., Siegert W. Human herpesvirus type 6 variants identified by single strand conformation polymorphism (SSCP) analysis. J. Virol. Meth. 73:1998;21-29.