Molecular analysis of expression of the lantibiotic Pep5 immunity phenotype

Applied and Environmental Microbiology

Volumn 65, Issue 2, 1999, Pages 591-598

  Pag, Ulrike ^a   Heidrich, Christoph ^a,b   Bierbaum, Gabriele ^a   Sahl, Hans Georg ^a  

^a UNIVERSITY OF BONN   (Germany)

^b MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LANTIBIOTIC; MESSENGER RNA; XYLOSE;

ARTICLE; GENE CLUSTER; IMMUNITY; IMMUNOBLOTTING; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS EPIDERMIDIS; START CODON; STRUCTURAL GENE;

POSIBACTERIA; PROKARYOTA; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 0032974997     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.2.591-598.1999     Document Type: Article

References (50)

1. Allgaier, H., G. Jung, R. G. Werner, U. Schneider, and H. Zähner. 1986. Epidermin: sequencing of a heterodet tetracyclic 21-peptide amide antibiotic. Eur. J. Biochem. 160:9-22.
2. Augustin, J., and F. Götz. 1990. Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation. FEMS Microbiol. Lett. 66:203-208.
3. Augustin, J., R. Rosenstein, B. Wieland, U. Schneider, N. Schnell, G. Engelke, K.-D. Entian, and F. Götz. 1992. Genetic analysis of epidermin biosynthetic genes and epidermin-negative mutants of Staphylococcus epidermidis. Eur. J. Biochem. 204:1149-1154.
4. Belasco, J. G., and C. F. Higgins. 1988. Mechanisms of mRNA decay in bacteria: a perspective. Gene 72:15-23.
5. Bierbaum, G., M. Reis, C. Szekat, and H.-G. Sahl. 1994. Construction of an expression system for engineering of the lantibiotic Pep5. Appl. Environ. Microbiol. 60:4332-4338.
6. Bierbaum, G., C. Szekat, M. Josten, C. Heidrich, C. Kempter, G. Jung, and H.-G. Sahl. 1997. Engineering of a novel thioether bridge and rule of modified residues in the lantibiotic Pep5. Appl. Environ. Microbiol. 62:385-392.
7. Bouvet, P., and J. G. Belasco. 1992. Control of RNase E-mediated RNA degradation by 5′-terminal base pairing in E. coli. Nature 360:488-491.
8. Brötz, H., M. Josten, I. Wiedemann, U. Schneider, F. Götz, G. Bierbaum, and H.-G. Sahl. 1998. Role of lipid-bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics. Mol. Microbiol. 30:317-327.
9. Chan, P. T., H. Ohmori, J. Tomizawa, and J. Lebowitz. 1985. Nucleotide sequence and gene organisation of ColE1 DNA. J. Biol. Chem. 260:8925-8935.
10. Chen, C.-Y. A., J. T. Beatty, S. N. Cohen, and J. G. Belasco. 1988. An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability. Cell 52:609-619.
11. Dodd, H. M., N. Horn, W. C. Chan, C. J. Giffard, B. W. Bycroft, G. C. K. Roberts, and M. J. Gasson. 1996. Molecular analysis of the regulation of nisin immunity. Microbiology 142:2385-2392.
12. Emory, S. A., P. Bouvet, and J. G. Belasco. 1992. A 5′-terminal stem-loop structure can stabilize mRNA in Escherichia coli. Genes Dev. 6:135-148.
13. Engelke, G., Z. Gutowski-Eckel, P. Kiesau, K. Siegers, M. Hammelmann, and K.-D. Entian. 1994. Regulation of nisin biosynthesis and immunity in Lactococcus lactis 6F3. Appl. Environ. Microbiol. 60:814-825.
14. Ersfeld-Dreßen, H., H.-G. Sahl, and H. Brandis. 1984. Plasmid involvement in production of and immunity to the staphylococcin-like peptide Pep5. J. Gen. Microbiol. 130:3029-3035.
15. Feliciello, I., and G. Chinali. 1993. A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal. Biochem. 212:394-401.
16. Geli, V., D. Baty, F. Pattus, and C. Lazdunski. 1989. Topology and function of the integral membrane protein conferring immunity to colicin A. Mol. Microhiol. 3:679-687.
17. Götz, F., and B. Schumacher. 1987. Improvements of protoplast transformation in Staphylococcus carnosus. FEMS Microbiol. Lett. 40:285-288.
18. Gross, E., H. Kiltz, and E. Nebelin. 1973. Subtilin. VI. Die Struktur des Subtilins. Hoppe-Seyler's Z. Physiol. Chem. 354:810-812.
19. Gross, E., and J. L. Morell. 1971. The structure of nisin. J. Am. Chem. Soc. 93:4634-4635.
20. Heidrich, C., U. Pag, M. Josten, J. Metzger, R. W. Jack, G. Bierbaum, G. Jung, and H.-G. Sahl. 1998. Isolation, characterization, and heterologous expression of the novel lantibiotic epicidin 280 and analysis of its biosynthetic gene cluster. Appl. Environ. Microbiol. 64:3140-3146.
21. Hue, K. K., S. D. Cohen, and D. H. Bechhofer. 1995. A polypurine sequence that acts as a 5′ mRNA stabilizer in Bacillus subtilis. J. Bacteriol. 177:3465-3471.
22. Kaletta, C., K.-D. Entian, R. Kellner, G. Jung, M. Reis, and H.-G. Sahl. 1989. Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence. Arch. Microbiol. 152:16-19.
23. Klein, C., and K.-D. Entian. 1994. Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633. Appl. Environ. Microbiol. 60:2793-2801.
24. Klug, G., and S. N. Cohen. 1990. Combined actions of multiple hairpin loop structures and sites of rate-limiting endonucleolytic cleavage determine differential degradation rates of individual segments within polycistronic puf operon mRNA. J. Bacteriol. 172:5140-5146.
25. Kuipers, O. P., M. M. Beerthuyzen, P. G. G. A. de Ruyter, E. J. Luesink, and W. M, de Vos. 1995. Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction. J. Biol. Chem. 270:27299-27304.
26. Kuipers, O. P., M. M. Beerthuyzen, R. J. Siezen, and W. M. de Vos. 1993. Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis: requirement of expression of the nisA and nisI genes for producer immunity. Eur. J. Biochem. 216:281-291.
27. McMaster, G. K., and G. G. Carmichael. 1977. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc. Natl. Acad. Sci. USA 74:4835-4838.
28. Meyer, C., G. Bierbaum, C. Heidrich, M. Reis, J. Süling, M. I. Iglesias-Wind, C. kempter, E. Molitor, and H.-G. Sahl. 1995. Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. Eur. J. Biochem. 232:478-489.
29. Peschel, A., and F. Götz. 1996. Analysis of the Staphylococcus epidermidis genes epiF, -E, and -G involved in epidermin immunity. J. Bacteriol. 178: 531-536.
30. Peterson, C. 1992. Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non-nucleolytic inactivation. Mol. Microbiol. 6:277-282.
31. Pugsley, A. P. 1987. Nucleotide sequencing of the structural gene for colicin N reveals homology between the catalytic, C-terminal domains of colicins A and N. Mol. Microbiol. 1:317-325.
32. Reis, M., M. Eschbach-Bludau, M. I. Iglesias-Wind, T. Kupke, and H.-G. Sahl. 1994. Producer immunity towards the lantibiotic Pep5: identification of the immunity gene pepI and localization and functional analysis of its gene product. Appl. Environ. Microbiol. 60:2876-2883.
33. Reis, M., and H.-G. Sahl. 1991. Genetic analysis of the producer self-protection mechanism ("immunity") against Pep5, p. 320-332. In G. Jung and H.-G. Sahl (ed.), Nisin and novel lantibiotics. Escom, Leiden, The Netherlands.
34. Rince, A., A. Dufour, P. Uguen, J.-P. Le Pennec, and D. Haras. 1997. Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity. Appl. Environ. Microbiol. 63:4252-4260.
35. Rosenberg, M., and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet. 13: 319-353.
36. Sahl, H.-G. 1991. Pore formation in bacterial membranes by cationic lantibiotics, p. 347-358. In G. Jung and H.-G. Sahl (ed.), Nisin and novel lantibiotics. Escom, Leiden, The Netherlands.
37. Sahl, H.-G., and G. Bierbaum. 1998. Lantibiotics: biosynthesis and biological activities of uniquely modified peptides from Gram-positive bacteria. Annu. Rev. Microbiol. 52:41-79.
38. Sahl, H.-G., and H. Brandis. 1981. Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis. J. Gen. Microbiol. 127:377-384.
39. Sahl, H.-G., M. Reis, M. Eschbach, C. Szekat, A. G. Beck-Sickinger, J. Metzger, S. Stevanovic, and G. Jung. 1991. Isolation of Pep5 prepeptides in different stages of modification, p. 332-346. In G. Jung and H.-G. Sahl (ed.). Nisin and novel lantibiotics, Escom, Leiden, The Netherlands.
40. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
41. Schleifer, K. H., and U. Fischer. 1982. Description of a new species of the genus Staphylococcus: Staphylococcus carnosus. Int. J. Syst. Bacteriol. 32: 153-156.
42. Schramm, E., T. Ölschläger, W. Tröger, and V. Braun. 1988. Sequence, expression and localization of the immunity protein for colicin B. Mol. Gen. Genet. 21:176-182.
43. Siegers, K., and K.-D. Entian. 1995. Genes involved in immunity to the lantibiotic nisin produced by Lactococcus lactis 6F3. Appl. Environ. Microbiol. 61:1082-1089.
44. Skaugen, M., C. I. M. Abildgaard, and I. F. Nes. 1997. Organization and expression of a gene cluster involved in the biosynthesis of the lantibiotic lactocin S. Mol. Gen. Genet. 253:674-686.
45. Song, H. Y., and W. A. Cramer. 1991. Membrane topography of CoIE1 gene products: the immunity protein. J. Bacteriol. 173:2935-2943.
46. Wieland, K.-P., B. Wieland, and F. Götz. 1995. A promoter-screening plasmid and xylose-inducible, glucose-repressible expression vectors for Staphylococcus carnosus. Gene 158:91-96.
47. Wong, H. C., and S. Chang. 1986. Identification of a positive retroregulator that stabilizes mRNAs in bacteria. Proc. Natl. Acad. Sci. USA 83:3233-3237.
48. Worobo, R. W., M. J. van Belkum, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1995. A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens. J. Bacteriol. 177:3143-3149.
49. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-109.
50. Zell, R., and H. Fritz. 1987. DNA mismatch repair in Escherichia coli counteracting the hydrolytic dcamination of 5-mcthyl-cytosine residues. EMBO J. 6:1809-1815.