Misdiagnosis of toxoplasma infection by PCR: Fears unfounded

Journal of Clinical Pathology

Volumn 52, Issue 6, 1999, Pages 468-470

  Bhalla, M ^a   Johnson, J D ^b   Holliman, R E ^b   Savva, D ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b ST GEORGE S HOSPITAL   (United Kingdom)

Author keywords

Cross reaction; Polymerase chain reaction; Toxoplasma gondii

Indexed keywords

BACTERIAL DNA; PROTOZOAL DNA;

ACTINOMYCES; ARTICLE; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC ERROR; MYCOBACTERIUM; NOCARDIA ASTEROIDES; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMODIUM FALCIPARUM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TOXOPLASMA GONDII; TOXOPLASMOSIS;

EID: 0032974068     PISSN: 00219746     EISSN: None     Source Type: Journal    
DOI: 10.1136/jcp.52.6.468     Document Type: Article

References (10)

1. Burg JL, Grover CM, Ponletty P, et al. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 1989;27:1787-92.
2. Savva D, Morris JC, Johnson JD, et al. Polymerase chain reaction for detection of Toxoplasma gondii. J Med Microbiol 1990;32:25-31.
3. Weiss LM, Uden SA, Salgo M, et al. Sensitive and specific detection of toxoplasma DNA in an experimental murine model: use of Toxoplasma gondii-specific cDNA and the polymerase chain reaction. J Infect Dis 1991;163:180-6.
4. Johnson JD, Butcher PD, Savva D, et al. Application of the polymerase chain reaction to the diagnosis of human toxoplasmosis. J Infect 1993;26:147-58.
5. McHugh TD, Ramsay AR, James EA, et al. Pitfalls of PCR: misdiagnosis of cerebral nocardia infection. Lancet 1995; 346:1436.
6. Holliman RE, Patel B, Johnson JD, et al. Pitfalls of PCR: misdiagnosis of cerebral nocardia infection. Lancet 1996; 347:335-6.
7. Ausubel FM, Brent R, Kingston RE, et al. Current protocols in molecular biology. New York: John Wiley and Sons, 1994.
8. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning, A laboratory manual. 2nd ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1989.
9. Ellis JT. Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii. Int J Parasitol 1998;28:1053-60.
10. Burg JL, Perelman D, Kasper LH, et al. Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol 1988,141:3584-91.