Site-specific fluorescence labelling of recombinant polyomavirus-like particles

Biological Chemistry

Volumn 380, Issue 3, 1999, Pages 397-401

  Schmidt, Uli ^a   Kenklies, Janet ^a   Rudolph, Rainer ^a   Böhm, Gerald ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Fluorescence marker; Gene therapy; In vitro assembly; Polyomavirus; VP1

Indexed keywords

CYSTEINE; FLUORESCEIN; FLUORESCENT DYE; MALEIMIDE; RECOMBINANT PROTEIN; SERINE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; ESCHERICHIA COLI; FLUORESCENCE; GENE THERAPY; NONHUMAN; POLYOMA VIRUS; PRIORITY JOURNAL; VIRION; VIRUS CELL INTERACTION; VIRUS RECOMBINANT;

ESCHERICHIA COLI; POLYOMAVIRUS;

EID: 0032971589     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.1999.053     Document Type: Article

References (16)

1. Anderson, W.F. (1998). Human gene therapy. Nature 392 Supp., 25-30.
2. Bartlett, J.S., and Samulski, R.J. (1998). Fluorescent viral vectors: A new technique for the pharmacological analysis of gene therapy. Nature Med. 4, 635-637.
3. Chen, X.S., Stehle, T., and Harrison, S.C. (1998). Interaction of polyomavirus internal protein VP2 with the major capsid protein VP1 and implications for participation of VP2 in viral entry. EMBO J. 17, 3233-3240.
4. Eckhart, W. (1991). Polyomaviridae and their replication. In: Fundamental Virology, 2nd ed. B.N. Fields and D.M. Knipe eds., (New York: Raven Press), pp. 727-741.
5. Forstova, J., Krauzewicz, N., Sandig, V., Elliott, J., Palkova, Z., Strauss, M., and Griffin, B.E. (1995). Polyomavirus pseudocapsids as efficient carriers of heterologous DNA into mammalian cells. Hum. Gene Ther. 3, 297-306.
6. Kraulis, P.J. (1991). Molscript: a program to produce both detailed and schematic plots of protein structures. J. Appl. Cryst. 24, 946-950.
7. Leopold, P.L., Ferris, B., Grinberg, I., Worgall, S., Hackett, N.R., and Crystal, R.G. (1998). Fluorescent virions: Dynamic tracking of the pathway of adenoviral gene transfer vectors in living cells. Hum. Gene Ther. 9, 367-378.
8. Li, M., Beard, P., Estes, P.A., Lyon, M.K., and Garcea, R.L. (1998). Intercapsomeric disulfide bonds in papillomavirus assembly and disassembly. J. Virol. 72, 2160-2167.
9. Mackay, R.L., and Consigli, R.A. (1976). Early events in polyomavirus infection: attachment, penetration and nuclear entry. J. Virol. 19, 620-636.
10. Merritt, E.A., and Bacon, D.J. (1997). Raster3D: Photorealistic molecular graphics. Methods Enzymol 277, 505-524.
11. Salunke, D.M., Caspar, D.L.D., and Garcea, R.L. (1986). Self-assembly of purified polyomavirus capsid protein VP1. Cell 46, 895-904.
12. Salunke, D.M., Caspar, D.L., and Garcea, R.L. (1989). Polymorphism in the assembly of polyomavirus capsid protein VP1. Biophys J. 56, 887-900.
13. Slilaty, S.N., Berns, K.I., and Aposhian, H.V. (1982). Polyoma-like particles: characterisation of the DNA encapsidated in vitro by polyoma empty capsids. J. Biol. Chem. 257, 6571-6575.
14. Stehle, T., and Harrison, S.C. (1997). High-resolution structure of a polyomavirus VP1-oligosaccharide complex: implications for assembly and receptor binding. EMBO J. 16, 5139-5148.
15. Stehle, T., Yan, Y., Benjamin, T.L, and Harrison, S.C. (1994). Structure of murine polyomavirus complexed with an oligosaccharide receptor fragment. Nature 369, 160-163.
16. Stehle, T., Gamblin, S.J., Yan, Y., and Harrison, S.C. (1996). The structure of simian virus 40 refined at 3.1 Å resolution. Structure 4, 165-182.