Overexpression and simple purification of human superoxide dismutase (SOD1) in yeast and its resistance to oxidative stress

Journal of Biotechnology

Volumn 68, Issue 1, 1999, Pages 29-35

  Yoo, Hae Yong ^a   Kim, Shin Seog ^a   Rho, Hyune Mo ^a  

^a Seoul National University   (South Korea)

Author keywords

Human Cu Zn SOD; Overexpression; Oxidative stress; Purification; Yeast

Indexed keywords

ALDEHYDES; AMMONIUM COMPOUNDS; COLUMN CHROMATOGRAPHY; ENZYMES; EXTRACTION; METHANOL; OXIDATION RESISTANCE; PLANT CELL CULTURE; PURIFICATION; YEAST;

AMMONIUM SULFATE; CELLULOSE COLUMN CHROMATOGRAPHY; HUMAN SUPEROXIDE DISMUTASE; OVEREXPRESSION; OXIDATIVE STRESS;

CLONING;

COPPER ZINC SUPEROXIDE DISMUTASE; DIETHYLAMINOETHYL CELLULOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ARTICLE; COLUMN CHROMATOGRAPHY; ENZYME PURIFICATION; EXPRESSION VECTOR; HUMAN; MOLECULAR CLONING; NONHUMAN; OXIDATIVE STRESS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT PLASMID; STRUCTURAL GENE; YEAST;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HEAT-SHOCK RESPONSE; HUMANS; OXIDATIVE STRESS; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SUPEROXIDE DISMUTASE; TRANSFORMATION, GENETIC;

EID: 0032971130     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00188-6     Document Type: Article

References (32)

1. Beauchamp C., Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. Anal. Biochem. 44:1971;276-287.
2. Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S., Stinchcomb D.T., Struhl K., Davis R.W. Sterile host yeasts (SHY4): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 8:1979;17-24.
3. Briggs R.G., Fee J.A. Further characterization of human erythrocyte superoxide dismutase. Biochim. Biophys. Acta. 537:1978;86-99.
4. Deisseroth A., Dounce A.L. Catalase: physical and chemical properties, mechanism of catalysis, and physiological role. Physiol. Rev. 50:1970;319-375.
5. Flohe L., Giertz H., Beckman R. Handbook of Inflammation. 5:1985;Elsevier Science, New York.
6. Forman H.J., Fridovich I. On the stability of bovine superoxide dismutase. J. Biol. Chem. 248:1973;2645-2649.
7. Fridovich I. Superoxide dismutase. Annu. Rev. Biochem. 44:1975;147-159.
8. Fridovich I. Superoxide radical: an endogenous toxicant. Annu. Rev. Pharmacol. Toxicol. 23:1983;239-257.
9. Friedman S.M. Biochemistry of Thermophily. 1978;Academic Press, New York.
10. Hallewell R.A., Mills R., Tekamp-Olson P., Blacher R., Rosenberg S., Otting F., Masiarz F.R., Scandella C.J. Amino terminal acetylation of authentic human CuZn superoxide dismutase produced in yeast. Biotechnology. 5:1987;363-366.
11. Hartman J.R., Geller T., Yavin Z., Bartfeld D., Kanner D., Aviv H., Gorecki M. High-level expression of enzymatically active human Cu/Zn superoxide dismutase in Escherichia coli. Proc. Natl. Acad. Sci. USA. 83:1986;7142-7146.
12. Hartz J.W., Deutsch H.F. Subunit structure of human superoxide dismutase. J. Biol. Chem. 247:1972;7043-7050.
13. Hill J., Ian K.A., Donald G., Griffiths D.E. DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res. 20:1991;5719.
14. Jabusch J.R., Farb D.L., Kerschensteiner D.A., Deutsch H.F. Some sulfhydryl properties and primary structure of human erythrocyte superoxide dismutase. Biochemistry. 19:1980;2310-2316.
15. Jamieson D.J., Stephen D.W., Terriere E.C. Analysis of the adaptive oxidative stress response of Candida albicans. FEMS Microbiol. Lett. 138:1996;83-88.
16. 2 extracted from a medicinal plant, Panax ginseng. J. Biol. Chem. 271:1996;24539-24543.
17. Kumagai Y., Shinyashiki M., Sun G.F., Shimojo N., Sagai M. An efficient method for purification of cuprozinc superoxide dismutase from bovine erythrocytes. Experientia. 50:1994;673-676.
18. Lee W.G., Kim Y.H., Yang J., Rho H.M. Molecular cloning and high-level expression of human cytoplasmic SOD gene in E. coli. Korean J. Microbiol. 28:1990;91-97.
19. Lieman-Hurwitz J., Dafni N., Lavie V., Groner Y. Human cytoplasmic superoxide dismutase cDNA clone: a probe for studying the molecular biology of Down syndrome. Proc. Natl. Acad. Sci. USA. 79:1982;2808-2811.
20. Liu X.D., Thiele D.J. Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription. Genes Dev. 10:1996;592-603.
21. Malinowski D.P., Fridovich I. Subunit association and side chain reactivities of bovine erythrocyte SOD in denaturing solvents. Biochemistry. 18:1979;5055-5060.
22. McCord J.M. Free radicals and inflammation: protection of synovial fluid by superoxide dismutase. Science. 185:1974;529-531.
23. McCord J.M., Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244:1969;6049-6055.
24. Moradas-Ferreira P., Costa V., Piper P., Mager W. The molecular defences against reactive oxygen species in yeast. Mol. Microbiol. 19:1996;651-658.
25. Oberley L.W., Buettner G.R. Role of superoxide dismutase in cancer: a review. Cancer Res. 39:1979;1141-1149.
26. Orr W.C., Sohal R.S. Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. Science. 263:1994;1128-1130.
27. Reddy M.V., Gangadharam P.R. Heat shock treatment of macrophages causes increased release of superoxide anion. Infect. Immun. 60:1992;2386-2390.
28. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning - A Laboratory Manual. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
29. Sherman L., Dafni N., L-Hurwitz J., Groner Y. Nucleotide sequence and expression of human chromosome 21 encoded superoxide dismutase mRNA. Proc. Natl. Acad. Sci. USA. 80:1983;5465-5469.
30. Steinman H.M. The amino acid sequence of copper-zinc superoxide dismutase from Baker's yeast. J. Biol. Chem. 255:1980;6758-6765.
31. Stellwagon E., Wilgus H. Friedman S. Biochemistry of Thermophily. 1978;Academic Press, New York.
32. Tolmasoff J.M., Ono T., Cutler R.G. Superoxide dismutase: correlation with life-span and specific metabolic rate in primate species. Proc. Natl. Acad. Sci. USA. 77:1980;2777-2781.