Direct PCR of symbiotic fungi using microslides

BioTechniques

Volumn 26, Issue 3, 1999, Pages 454-455

  Wolinski, Heimo ^a   Grube, Martin ^a   Blanz, Paul ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA;

ARTICLE; FUNGUS GROWTH; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; GENE ISOLATION; LICHEN; POLYMERASE CHAIN REACTION; SYMBIOSIS;

FUNGI;

EID: 0032968932     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99263bm20     Document Type: Article

References (8)

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2. Gargas, A. and P.T. DePriest. 1996. A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA. Mycologia 88:745-748.
3. Gargas, A. and J. Taylor. 1992. Polymerase chain reaction (PCR) primers for amplifying and sequencing nuclear 18S rDNA from lichenized fungi. Mycologia 84:589-592.
4. Grube, M., A. Gargas, P.T. DePriest and J. Hafellner. 1995. DNA isolation from lichen ascomata. Mycol. Res. 99:1321-1324.
5. Hindkjaer, J., C. Terkelsen, S. Kølvraa, J. Koch and L. Bolund. 1996. Detection of nucleic acids (DNA and RNA) in situ by single and cyclic primed in situ labelling (PRINS): two alternatives to traditional in situ hybridization methods, p. 45-66. In M. Clark (Ed.), In Situ Hybridization. Chapman and Hall, London.
6. Lee, S.B. and J.W. Taylor. 1990. Isolation of DNA from fungal mycelia and single spores, p. 282-287. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego.
7. White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, p. 315-322. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego.
8. Yap, E.P.H. and J.O'D. McGee. 1991. Slide PCR: DNA amplification from cell samples on microscopic glass slides. Nucleic Acids Res. 19:4294.