Measurement of native dextran synthesis and sedimentation properties by analytical ultracentrifugation

Journal of Chemical Technology and Biotechnology

Volumn 74, Issue 1, 1999, Pages 17-24

  Setford, Steven J ^a  

^a CRANFIELD UNIVERSITY   (United Kingdom)

Author keywords

Bioreaction; Dextran; Sedimentation; Separation; Ultracentrifuge; Zonal

Indexed keywords

BIOSYNTHESIS; CATALYSIS; CENTRIFUGATION; ENZYMES; SEDIMENTATION; SUGAR (SUCROSE);

DEXTRAN; DEXTRANSUCRASE; SVEDBERGS SEDIMENTATION COEFFICIENTS; ULTRACENTRIFUGATION;

POLYSACCHARIDES;

DEXTRAN; DEXTRANSUCRASE; SUCROSE;

CENTRIFUGE; SEDIMENT; SEPARATION;

ARTICLE; BIOSYNTHESIS; DIFFUSION COEFFICIENT; MOLECULAR WEIGHT; SEDIMENTATION RATE; ULTRACENTRIFUGATION; VISCOSITY;

EID: 0032967402     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4660(199901)74:1<17::AID-JCTB983>3.0.CO;2-Q     Document Type: Article

References (36)

1. Shieh MT and Barker PE, Combined Bioreaction and Separation in a Simulated Countercurrent Chromatographic Bioreactor-Separator for the Hydrolysis of Lactose. J Chem Technol Biotechnol 66:265-278 (1996).
2. Shieh MT and Barker PE, Saccharification of modified starch to maltose in a semi-continuous countercurrent chromatographic reactor separator (SCCR-S). J Chem Technol Biotechnol 63:125-134 (1995).
3. Barker PE, Ganetsos G and Ajongwen NJ, A novel approach to the production of clinical-grade dextran. J Chem Technol Biotechnol 57:21-26 (1993).
4. Sarmidi MR and Barker PE, Simultaneous biochemical reaction and separation in a rotating annular chromatograph Chem Eng Sci 48:2615-2623 (1993).
5. Barker PE, Ganetsos G, Ajongwen J and Akintoye A, Bioreaction-Separation on Continuous chromatograph systems. Chem Eng Journal and Biochem Eng Journal 50:B23-28 (1992).
6. Zafar I and Barker PE, An experimental and computational study of a Biochemical polymerization in a chromatographic reactor separator. Chem Eng Sci 43:2369-2375 (1988).
7. Hashimoto K, Shuji A, Hiromitsu N and Ueda Y, A new Process combining adsorption and enzyme reaction for producing higher fructose syrup Biotechnol Bioeng 25:2371-2393 (1983).
8. Nilsson K and Soderlund G, Clinical dextrans specifications and quality of preparations on the market. Acta Pharmaceuticals Suec 15:434-454 (1978).
9. Alsop RM, Industrial production of dextrans, in Progress in Industrial Microbiology, ed by Bushell ME, Elsevier, Amsterdam. Vol 18, pp 1-45 (1983).
10. Robyt JF and Walseth TF, The mechanism of acceptor reaction of Leuconstoc mesenteroides B-512F dextransucrase. Carbohyd Res 61:433-445 (1978).
11. Setford SJ, Barker PE and Ganetsos G, Combined bioreaction and separation in centrifugal fields. Bioseparation 4:117-126 (1994).
12. Setford SJ and Barker PE, Combined bioreaction and separation in centrifugal fields using zonal rotors. J. Chem Technol Biotechnol 61:19-30 (1994).
13. Cohen R, Giraud B and Messiah, A, Theory and practice of the ultracentrifugation of an active enzyme-substrate complex. Biopolymers 5:203-225 (1967).
14. Parts AG and Elbing E, The polymerisation of Acrylonitrile in centrifugal fields. Am Chem Soc Polym Prepr 16:211-216 (1975).
15. Gilbert GA, Sedimentation and electrophoresis of interacting substances. Proc Royal Soc London A250:377-388 (1959).
16. Schumaker VN, Zone centrifugation Adv Bio Med Phys 11: 245-339 (1967).
17. Williams JW and Saunders WM, Size distribution analysis in plasma extender systems. II. Dextran J Phys Chem 58: 854-859 (1954).
18. Ogston JE and Woods, EF, The sedimentation of some fractions of degraded dextran. Trans Faraday Soc 50:635-643 (1954).
19. Barker PE and Ajongwen NJ, The production of the enzyme dextransucrase using non-aerated fermentation techniques. Biotech Bioeng 37: 703-707 (1991).
20. Ajongwen JN, Akintoye A, Barker PE, Ganetsos G and Shieh MT, Large-scale purification of Leuconostoc mesenteroides NRRL-B512F dextransucrase for use in the biosynthesis of dextran by batch and continuous chromatography. Chem Eng Journal and Biochem Eng Journal 51:B43-50 (1993).
21. Alsop RM and Vlachogiannis GJ, Determination of the molecular weight of clinical dextran by gel permeation chromatography on TSK-PW type columns. J Chromatog 246:227-240 (1982).
22. McCall JS and Potter BJ, Ultracentrifugation, Bailliere Tindall, London (1973).
23. MSE Technical Publication No 71. The MSE Analytical Ultracentrifuge Mk 2, Measuring and Scientific Equipment Ltd, Crawley, Sussex, UK.
24. Schachman HK, The Ultracentrifuge in Biochemistry, Academic Press, New York (1959).
25. Svedberg T and Pedersen KO, The Ultracentrifuge, Oxford University Press, Oxford (1940).
26. Rees DA and Scott WE, Polysaccharide conformation. Part 6. J Chem Soc (B) 469-479 (1971).
27. Bovey FA, Enzyme polymerisation I, molecular weight and branching during the formation of dextran J Polym Sci 35:167-183 (1959).
28. Tsuchiya HM, Hellman NN, Koepsell HJ, Corman J, Stringer CS, Rogovin SP, Bogard MO, Bryant G, Feder VH, Hoffman CA, Senti FR and Jackson RW, Factors affecting molecular weight of enzymatically synthesised dextran. J Am Chem Soc 77:2412-2419 (1955).
29. Ingelman B and Siegbahn K, Dextran and levan molecules studied with the electron microscope. Nature 154:237-238 (1944).
30. Weast RC (ed), The Handbook of Chemistry and Physics, 53rd Edn, Chemical and Rubber Company Press, Ohio (1972).
31. Senti FR, Hellman NN, Ludwig NH, Babcock GE, Tobin R, Glass CA and Lamberts BL, Viscosity, sedimentation and light scattering properties of fractions of an acid-hydrolysed dextran, J Polym Sci 17:527-546 (1955).
32. Fujita H, Effects of concentration dependence on the sedimentation coefficient in velocity ultracentrifugation. J Chem Phys 24: 1084-1089 (1956).
33. Williams JW, Baldwin RL, Saunders WM and Squire PG, Boundary Spreading in sedimentation velocity experiments. I. The enzymatic degradation of serum globulins. J Am Chem Soc 74:1542-1547 (1952).
34. Baldwin RL and Van Holde KE, Sedimentation of high polymers. Adv Polym Sci 1:451-511 (1960).
35. Chervenka CH, A Manual of Methods for the Analytical Ultracentrifuge, Beckman Instruments Ltd, Spinco Division, Palo Alto, California (1973).
36. Setford SJ, Combined bioreaction and separation in centrifugal fields, PhD thesis, Aston University, Birmingham, UK (1992).