C-terminal region of hTPO is important for secretion and expression in insect cells

Biochemistry and Molecular Biology International

Volumn 47, Issue 5, 1999, Pages 729-733

  Ahn, Hyea Kyung ^a   Chung, Joo Young ^a   Park, Sang Kyu ^a   Joo, Sang Myung ^a   Park, Seung Kook ^a   Koh, Yeo Wook ^a  

^a Daewoong Pharmaceutical Co Ltd   (South Korea)

Author keywords

Expression; Secretion; Truncated hTPO

Indexed keywords

COMPLEMENTARY DNA; THROMBOPOIETIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CELL; CULTURE MEDIUM; GENE EXPRESSION SYSTEM; GLYCOSYLATION; IMMUNOBLOTTING; INSECT; MOLECULAR SIZE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN SECRETION;

ANIMALS; BACULOVIRIDAE; CELLS, CULTURED; DNA, COMPLEMENTARY; GENE EXPRESSION; GLYCOSYLATION; HUMANS; INSECTS; POLYMERASE CHAIN REACTION; THROMBOPOIETIN; TRANSFECTION;

ANIMALIA; INSECTA; TRICHOPLUSIA NI; UNIDENTIFIED BACULOVIRUS;

EID: 0032966951     PISSN: 10399712     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. De Sauvage, F. J., Hass, P. E., Spencer, S. D., Malloy, B. E., Gurney, A. L., Spencer, S. A., Darbonne, W. C., Henzel, W. J., Wong, S C., Kuang, W. J., Oles, K. J., Hutgren, B., Solberg, L. A., Goeddel, D. V., and Eaton, D. L. (1994) Nature 369, 533-538.
2. Lok, S., Kaushansky, K., Holly, R. D., Kuijper, J. L., Lofton-Day, C. E., Oort, P. J., Grant, F. J., Heipel, M. D., Burkhead, S. K , Kramer, J. M, Bell, L. A., Sprecher, C. A., Blumberg, H., Jonson, R., Prunkard, D., Ching, A. F. T., Mathewes, S. L., Bailey, M. C., Forstrom, J. W., Buddle, M. M., Osborn, S. G., Evans, S. J., Sheppard, P. O., Presnell, S. R., O'Hara, P. J., Hagen, F. S., Roth, G., and Foster, D. C. (1994) Nature 369, 565-568.
3. Kaushansky, K., Lok, S., Holy, R. D , Broudy, V. C., Lin, N., Bailey, M. C., Forstrom, J. W., Buddle, M. M., Oort, P. J., Hagen, F. S., Roth, G. J., Papayannopoulou, T., and Foster, D. C. (1994) Nature 369, 568-571.
4. Solar, G. P., Kerr, W. G., Zeigler, F. C., Fess, D., Donahue, C., de Sauvage, F. J., and Eaton, D. L. (1998) Blood 92, 4-10.
5. Wada, T., Nagta, Y., Nagahisa, H., Okutomi, K., Ha, S. H., Ohnuki, T., Kanaya, T., Matsumura, M., and Todokoro, K. (1995) Biochem. Biophy. Res. Comm. 213, 1091-1098.
6. Sohma, Y., Akahori, H., Seki, N., Hori, T., Ogami, K., Kato, T., Shimada, Y., Kawamura, K., and Miyazaki, H. (1994) FEBS Lett. 353, 57-61.
7. Eriksen, S. H., Jensen, B., and Olsen, J. (1998) Curr. Microbial. 37, 117-122.
8. Jenkins, N., Parekh, R. B., and James, D. C. (1996) Nature. Biotechnol. 14, 975-981.
9. Hou, J., and Jhan, H. (1998) Cytokine 10, 319-330.