Genetic interactions between KAR7/SEC71, KAR8/JEM1, KAR5, and KAR2 during nuclear fusion in Saccharomyces cerevisiae

Molecular Biology of the Cell

Volumn 10, Issue 3, 1999, Pages 609-626

  Brizzio, Valeria ^a,b   Khalfan, Waheeda ^a   Huddler, Don ^a   Christopher T, Beh ^a,c   Andersen, Søren S L ^a,d   Latterich, Martin ^e   Rose, Mark D ^a  

^a PRINCETON UNIVERSITY   (United States)

^b MILLENNIUM PHARMACEUTICALS INC   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELISM; ARTICLE; CELL NUCLEUS MEMBRANE; COMPLEX FORMATION; GENE FUNCTION; GENE OVEREXPRESSION; GENE TRANSLOCATION; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0032963615     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.10.3.609     Document Type: Article

References (59)

1. Beh, C.T. (1996). Functions of the Yeast Endoplasmic Reticulum. Ph.D. Thesis. Princeton, NJ: Princeton University.
2. Beh, C.T., Brizzio, V., and Rose, M.D. (1997). KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion. J. Cell Biol. 139, 1063-1076.
3. Brizzio, V., Gammie, A.E., Nijbroek, G., Michaelis, S., and Rose, M.D. (1996). Cell fusion during yeast mating requires high levels of a-factor mating pheromone. J. Cell Biol. 135, 1727-1739.
4. Brodsky, J.L., and Schekman, R. (1993). A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome. J. Cell Biol. 123, 1355-1363.
5. Byers, B. (1981). Multiple roles of the spindle pole bodies in the life cycle of Saccharomyces cerevisiae. In: Molecular Genetics of Yeast, ed. D. von Wittstein, J. Friis, M. Kielland-Brandt, and A. Stenderup, Copenhagen: Munksgaard, 119-131.
6. Byers, B., and Goetsch, L. (1975). Behavior of spindles and spindle plaques in the cell cycle and conjugation in Saccharomyces cerevisiae. J. Bacteriol. 124, 511-523.
7. Conde, J., and Fink, G.R. (1976). A mutant of Saccharomyces cervisiae defective for nuclear fusion. Proc. Natl. Acad. Sci. USA 73, 3651-3655.
8. Deshaies, R.J., Sanders, S.L., Feldheim, D.A., and Schekman, R. (1991). Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature 349, 806-808.
9. Deshaies, R.J., and Schekman, R. (1987). A yeast mutant defective at an early stage in import of secretory precursors into the endoplasmic reticulum. J. Cell Biol. 105, 633-645.
10. Deshaies, R.J., and Schekman, R. (1989). SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum. J. Cell Biol. 109, 2653-2664.
11. Erdman, S., Lin, L., Malczynski, M.D., and Snyder, M. (1998). Pheromone-regulated genes required for yeast mating differentiation. J. Cell Biol. 140, 461-483.
12. Esnault, Y., Feldheim, D., Blondel, M.O., Schekman, R., and Kepes, F. (1994). SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus. J. Biol. Chem. 269, 27478-27485.
13. Fang, H., and Green, N. (1994). Nonlethal sec71-1 and sec72-1 mutations eliminate proteins associated with the Sec63p-BiP complex from S. cerevisiae. Mol. Biol. Cell 5, 933-942.
14. Feldheim, D., Rothblatt, J., and Schekman, R. (1992). Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation. Mol. Cell. Biol. 12, 3288-3296.
15. Feldheim, D., and Schekman, R. (1994). Sec72p contributes to the selective recognition of signal peptides by the secretory polypeptide translocation complex. J. Cell Biol. 126, 935-943.
16. Feldheim, D., Yoshimura, K., Admon, A., and Schekman, R. (1993). Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum. Mol. Biol. Cell 4, 931-939.
17. Gammie, A., Brizzio, V., and Rose, M. (1998). Distinct morphological phenotypes of cell fusion mutants. Mol. Cell. Biol. 9, 1395-1410.
18. Gammie, A., Stewart, B.G., Scott, C.F., and Rose, M.D. (1999). The two forms of karyogamy transcription factor Kar4p are regulated by differential initiation of transcription, translation, and protein turnover. Mol. Cell. Biol. 19, 817-825.
19. Green, N., Fang, H., and Walter, P. (1992). Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae. J. Cell Biol. 116, 597-604.
20. Hanein, D., Matlack, K.E., Jungnickel, B., Plath, K., Kalies, K.U., Miller, K.R., Rapoport, T.A., and Akey, C.W. (1996). Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell 87, 721-732.
21. Herskowitz, I. (1995). MAP kinase pathways in yeast: for mating and more. Cell 80, 187-197.
22. Hoffman, C.S., and Winston, F. (1987). A ten minute DNA preparation from yeast releases autonomous plasmids for transformation of E. coli. Gene 57, 267-272.
23. Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983). Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
24. Kurihara, L.J., Beh, C.T., Latterich, M., Schekman, R., and Rose, M.D. (1994). Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway. J. Cell Biol. 126, 911-923.
25. Kurihara, T., and Silver, P. (1993). Suppression of a sec63 mutations identifies a novel component of the yeast endoplasmic reticulum translocation apparatus. Mol. Biol. Cell 4, 919-930.
26. Latterich, M., and Schekman, R. (1994). The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion. Cell 78, 87-98.
27. Lyman, S.K., and Schekman, R. (1995). Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae. J. Cell Biol. 131, 1163-1171.
28. Lyman, S.K., and Schekman, R. (1996). Polypeptide translocation machinery of the yeast endoplasmic reticulum. Experientia 52, 1042-1049.
29. Lyman, S.K., and Schekman, R. (1997). Binding of secretory precursor polypeptides to a translocon subcomplex is regulated by BiP. Cell 88, 85-96.
30. Marsh, L., and Rose, M.D. (1997). The pathway of cell and nuclear fusion during mating in S. cerevisiae. In: The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, ed. J.R. Pringle, J.R. Broach, and E.W. Jones, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 827-888.
31. Meluh, P.B., and Rose, M.D. (1990). KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60, 1029-1041.
32. Miller, R.K., and Rose, M.D. (1998). Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast. J. Cell Biol. 140, 377-390.
33. Nelson, M.K., Kurihara, T. and Silver, P. (1993). Extragenic suppressors of mutations in the cytoplasmic C-terminus of SEC63 define five genes in Saccharomyces cerevisiae. Genetics 134, 159-173.
34. Ng, D.T.W., and Walter, P. (1996). ER membrane protein complex required for nuclear fusion. J. Cell Biol. 132, 499-510.
35. Nishikawa, S., and Endo, T. (1997). The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion. J. Biol. Chem. 272, 12889-12892.
36. Nishikawa, S., and Endo, T. (1998). Reinvestigation of the functions of the hydrophobic segment of Jem1p, a yeast endoplasmic reticulum membrane protein mediating nuclear fusion. Biochem. Biophys. Res. Commun. 244, 785-799.
37. Ohashi, A., Gibson, J., Gregor, I., and Schatz, G. (1982). Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent. J. Biol. Chem. 257, 13042-13047.
38. Panzner, S., Dreier, L., Hartmann, E., Kostka, S., and Rapoport, T.A. (1995). Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p. Cell 81, 561-570.
39. Polaina, J., and Conde, J. (1982). Genes involved in the control of nuclear fusion during the sexual cycle of Saccharomyces cerevisiae. Mol. Gen. Genet. 186, 253-258.
40. Riles, L., Dutchik, J.E., Baktha, A., McCauley, B.K., Thayer, E.C., Leckie, M.P., Braden, V.V., Depke, J.E., and Olson, M.V. (1993). Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kb pairs. Genetics 134, 81-150.
41. Rose, M.D. (1996). Nuclear fusion in the yeast, Saccharomyces cerevisiae. Annu. Rev. Cell Dev. Biol. 12, 663-695.
42. Rose, M.D., Misra, L.M., and Vogel, J.P. (1989). KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene. Cell 57, 1211-1221.
43. Rose, M.D., Novick, P., Thomas, J.H., Botstein, D., and Fink, G.R. (1987). A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60, 237-243.
44. Rose, M.D., Winston, F., and Hieter, P. (1990). Methods of Yeast Genetics, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
45. Rothblatt, J.A., Deshaies, R.J., Sanders, S.L., Daum, G., and Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell Biol. 109, 2641-2652.
46. Sadler, I., Chiang, A., Kurihara, T., Rothblatt, J., Way, J., and Silver, P. (1989). A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J. Cell Biol. 109, 2665-2675.
47. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
48. Sanders, S.L., Whitfield, K.M., Vogel, J.P., Rose, M.D., and Schekman, R.W. (1992). Sec61p and BIP directly facilitate polypeptide translocation into the ER. Cell 69, 353-365.
49. Scherer, S., and Davis, R.W. (1979). Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc. Natl. Acad. Sci. USA 76, 4951-4955.
50. Schlenstedt, G., Harris, S., Risse, B., Lill, R., and Silver, P.A. (1995). A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s. J. Cell Biol. 129, 979-988.
51. Scidmore, M.A., Okamura, H.H., and Rose, M.D. (1993). Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum. Mol. Biol. Cell 4, 1145-1159.
52. Sikorski, R., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
53. Silberstein, S., Schlenstedt, G., Silver, P.A., and Gilmore R. (1998). A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum. J. Cell Biol. 143, 921-933.
54. Sprague, G.F.J., and Thorner, J.W. (1994). Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae. In: The Molecular and Cellular Biology of the Yeast Saccharomyces, ed. E.W. Jones, J.R. Pringle, and J.R. Broach, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 657-744.
55. Stearns, T., and Botstein, D. (1988). Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae. Genetics 119, 249-260.
56. Stirling, C.J., Rothblatt, J., Hosobuchi, M., Deshaies, R., and Schekman, R. (1992). Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell 3, 129-142.
57. Tange, Y., Horio, T., Shimanuki, M., Ding, D.Q., Hiraoka, Y., and Niwa, O. (1998). A novel fission yeast gene, tht1(+), is required for the fusion of nuclear envelopes during karyogamy. J. Cell Biol. 140, 247-258.
58. Vogel, J. (1993). KAR2, the Yeast Homologue of Mammalian BiP/ GRP78. Ph.D. Thesis, Princeton, NJ: Princeton University.
59. Vogel, J.P., Misra, L.M., and Rose, M.D. (1990). Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast. J. Cell Biol. 110, 1885-1895.