Xenopus laevis pancreatic DNase I: Purification and immunological characterization

Experimental and Clinical Immunogenetics

Volumn 16, Issue 2, 1999, Pages 117-123

  Hosomi, Osamu ^a   Yasuda, Toshihiro ^a   Takeshita, Haruo ^a   Nakajima, Tamiko ^a   Nakashima, Yoshimitsu ^a   Hanaoka, Yoichi ^b   Kishi, Koichiro ^a  

^a GUNMA UNIVERSITY   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

Antibody, specific; DNase I; Pancreas; Tissue distribution; Xenopus laevis

Indexed keywords

CALCIUM ION; DEOXYRIBONUCLEASE I; EDETIC ACID; MAGNESIUM ION; PANCREAS ENZYME;

AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; CATTLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME STRUCTURE; FEMALE; HUMAN; MOLECULAR WEIGHT; MOUSE; NONHUMAN; PH; PRIORITY JOURNAL; RABBIT; RECTUM; SEQUENCE HOMOLOGY; SHEEP; SWINE; TISSUE DISTRIBUTION; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; CATALYSIS; DEOXYRIBONUCLEASE I; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; FEMALE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNE SERA; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PANCREAS; RATS; SPECIES SPECIFICITY; XENOPUS LAEVIS;

ANIMALIA; ANURA; BOS TAURUS; BOVINAE; MAMMALIA; ORYCTOLAGUS CUNICULUS; OVIS; OVIS ARIES; SUIDAE; SUS SCROFA; XENOPUS LAEVIS;

EID: 0032963569     PISSN: 02549670     EISSN: None     Source Type: Journal    
DOI: 10.1159/000019102     Document Type: Article

References (26)

1. Moore S: Pancreatic DNase; in Boyer PD (ed): The Enzymes, ed 3. New York, Academic Press, 1981, vol 14, pp 281-296.
2. Liao T-H, Salnikow J, Moore S, Stein WH: Bovine pancreatic deoxyribonuclease A. J Biol Chem 1973; 248:1489-1495.
3. Paudel HK, Liao T-H: Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas. J Biol Chem 1986;61:16012-16017.
4. Lacks SA: Deoxyribonuclease I in mammalian tissues: Specificity of inhibition by actin. J Biol Chem 1981;256:2644-2648.
5. Takeshita H, Yasuda T, Nadano D, Iida R, Kishi K: Deoxyribonuclease I from rat urine: Affinity purification, characterization, and immunochemical studies. J Biochem 1995; 118:932-938.
6. Takeshita H, Yasuda T, Nakajima T, Hosomi O, Nakashima Y, Kishi K: Mouse deoxyribonuclease I (DNase I): Biochemical and immunological characterization, cDNA structure and tissue distribution. Biochem Mol Biol Int 1997;42:65-75.
7. Takeshita H, Yasuda T, Nadano D, Tenjo E, Sawazaki K, Iida R, Kishi K: Detection of deoxyribonucleases I and II (DNases I and II) activities in productive organs of male rabbits. Int J Biochem 1994; 26:1025-1031.
8. Yasuda T, Takeshita H, Nakajima T, Hosomi O, Nakashima Y, Kishi K: Rabbit DNase I: Purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships to other mammalian DNases I and phylogenetic analysis. Biochem J 1997;325:465-473.
9. Yasuda T, Awazu S, Sato W, Iida R, Tanaka Y, Kishi K: Human genetically polymorphic deoxyribonuclease: Purification, characterization and multiplicity of urine deoxyribonuclease I. J Biochem 1990;108: 393-398.
10. Peitsch MC, Polzar B, Stephan H, Crompton T, MacDonald HR, Mannherz HG, Tschopp J: Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death). EMBO J 1993;12:371-377.
11. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S: A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998; 391:43-50.
12. Kishi K, Yasuda T, Ikehara Y, Sawazaki K, Sato W, Iida R: Human serum deoxyribonuclease I (DNase I) polymorphism: Pattern similarities among isozymes from serum, urine, kidney, liver, and pancreas. Am J Hum Genet 1990;47:121-126.
13. Yasuda T, Nadano D, Iida R, Takeshita H, Lane SA, Callen DF, Kishi K: Chromosomal assignment of the human deoxyribonuclease I gene, DNASE 1 (DNL 1), to band 16p13.3 using the polymerase chain reaction. Cytogenet Cell Genet 1995;70:221-223.
14. Iida R, Yasuda T, Aoyama M, Tsubota E, Kobayashi M, Yuasa I, Matsuki T, Kishi K: The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism. Electrophoresis 1997;18:1936-1939.
15. Yasuda T, Kishi K, Yanagawa Y, Yoshida A: Structure of the human deoxyribonuclease I (DNase I) gene: Identification of the nucleotide substitution that generates its classical genetic polymorphism. Ann Hum Genet 1995;59:1-15.
16. Nadano D, Yasuda T, Kishi K: Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method. Clin Chem 1993;39: 448-452.
17. Yasuda T, Takeshita H, Nakazato E, Nakajima T, Hosomi O, Nakashima Y, Kishi K: Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence. Anal Biochem 1998;255: 274-276.
18. 4. Nature 1970;227:680-685.
19. Kishi K, Yasuda T, Awazu S, Mizuta K: Genetic polymorphism of human urine deoxyribonuclease I. Hum Genet 1989;81:295-297.
20. Yasuda T, Mizuta K, Ikehara Y, Kishi K: Genetic analysis of human deoxyribonuclease I by immunoblotting and zymogram method following isoelectric focusing. Anal Biochem 1989;183:84-88.
21. Nakajima T, Yasuda T, Nakashima Y, Hosomi O, Takeshita H, Kishi K: Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity. Immunol Invest 1998;27:145-152.
22. Shak S, Capon DJ, Hellmiss R, Marsters SA, Baker CL: Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum. Proc Natl Acad Sci USA 1990;87: 9188-9192.
23. Polzar B, Mannherz HG: Nucleotide sequence of a full length cDNA clone encoding the deoxyribonuclease I from the rat parotid gland. Nucleic Acids Res 1990;18:7151.
24. Peitsch MC, Irmler M, French LE, Tschopp J: Genomic organisation and expression of mouse deoxyribonuclease I. Biochem Biophys Res Commun 1995;207:62-68.
25. Polzar B, Zanotti S, Stephan H, Rauch F, Peitsch MC, Irmler M, Tschopp J, Mannherz HG: Distribution of deoxyribonuclease I in rat tissues and its correlation to cellular turnover and apoptosis (programmed cell death). Eur J Cell Biol 1994;64:200-204.
26. Yasuda T, Takeshita H, Sawazaki K, Nadano D, Iida R, Miyahara S, Kishi K: A new individualization marker of sweat: Deoxyribonuclease I (DNase I) polymorphism. J Forensic Sci 1996;41:862-864.