Quantification of tumour vasculature and hypoxia by immunohistochemical staining and HbO2 saturation measurements

British Journal of Cancer

Volumn 79, Issue 3-4, 1999, Pages 464-471

  Fenton, Bm ^a,c   Paoni, Sf ^a   Lee, J ^a   Koch, Cj ^b   Lord, Em ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Angiogenesis; Blood vessels; Hypoxic marker; Image analysis; Oxyhaemoglobin; Tumour oxygenation

Indexed keywords

CD31 ANTIGEN; FLUORESCENT DYE; HOE 33342; MONOCLONAL ANTIBODY; OXYHEMOGLOBIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD OXYGEN TENSION; COMPUTER ANALYSIS; FROZEN SECTION; HYPOXIA; IMAGE ANALYSIS; IMMUNOCHEMISTRY; METHODOLOGY; MICROSCOPY; MOUSE; NONHUMAN; OXYGEN CONSUMPTION; OXYGEN DIFFUSION; PRIORITY JOURNAL; SPECTROPHOTOMETRY; TUMOR VASCULARIZATION; VIDEORECORDING;

ANIMALS; ANOXIA; ANTIBODIES, MONOCLONAL; BIOLOGICAL TRANSPORT; BLOOD VESSELS; FEMALE; FLUORESCENT DYES; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MICE; NEOPLASMS, EXPERIMENTAL; NEOVASCULARIZATION, PATHOLOGIC; OXYGEN; OXYHEMOGLOBINS; SARCOMA, EXPERIMENTAL;

EID: 0032961720     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjc.6690072     Document Type: Article

References (20)

1. Brown JM and Giaccia AJ (1994) Tumour hypoxia: the picture has changed in the 1990s. (Review). Int J Radiat Biol 65: 95-102
2. Fenton BM and Gayeski TEJ (1990) Determination of microvascular oxyhemoglobin saturations using cryospectrophotometry. Am J Physiol 259: H1912-H1920
3. Fenton BM and Way BW (1993) Vascular morphometry of KHT and RIF-1 murine sarcomas. Radiother Oncol 28: 57-62
4. Fox SB, Leek RD, Weekes MP, Whitehouse RM, Gatter KC and Harris AL (1995) Quantitation and prognostic value of breast cancer angiogenesis: comparison of microvessel density, Chalkley count, and computer image analysis. J Pathol 177: 275-283
5. Horsman MR, Nordsmark M, Khalil AA, Hill SA, Chaplin DJ, Siemann DW and Overgaard J (1994) Reducing acute and chronic hypoxia in tumours by combining nicotinamide with carbogen breathing. Acta Oncologica 33: 371-376
6. Huang X, Molema G, King S, Watkins L, Edgington TS and Thorpe PE (1997) Tumor infarction in mice by antibody-directed targeting of tissue factor to tumour vasculature. Science 275: 547-550
7. Kayar SR, Archer PG, Lechner AJ and Banchero N (1982) The closest-individual method in the analysis of the distribution of capillaries. Microvasc Res 24: 326-341
8. Klauber N, Parangi S, Flynn E, Hamel E and Damato RJ (1997) Inhibition of angiogenesis and breast cancer in mice by the microtubule inhibitors 2-methoxyestradiol and taxol. Cancer Res 57: 81-86
9. Loats JT, Sillau AH and Banchero N (1978) How to quantify skeletal muscle capillarity. Adv Exp Med Biol 94: 41-48
10. Lord EM, Harwell L and Koch CJ (1993) Detection of hypoxic cells by monoclonal antibody recognizing 2-nitroimidazole adducts. Cancer Res 53: 5721-5726
11. Olive PL and Durand RE (1987) Characterization of a carbocyanine derivative as a fluorescent penetration probe. Cytometry 8: 571-575
12. O'Reilly MS, Boehm T, Shing Y, Fukai N, Vasios G, Lane WS, Flynn E, Birkhead JR, Olsen BR and Folkman J (1997) Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88: 277-285
13. Rijken PFJW, Bernsen HJJA and van der Kogel AJ (1995) Application of an image analysis system to the quantitation of tumor perfusion and vascularity in human glioma xenografts. Microvasc Res 50: 141-153
14. Smith KA, Hill SA, Begg AC and Denekamp J (1988) Validation of the fluorescent dye Hoechst 33342 as a vascular space marker in tumours. Br J Cancer 57: 247-253
15. Thomson JE and Rauth AM (1974) An in vitro assay to measure the viability of KHT tumor cells not previously exposed to culture conditions. Radiat Res 58: 262-276
16. Trotter MJ, Chaplin DJ, Durand RE and Olive PL (1989a) The use of fluorescent probes to identify regions of transient perfusion in murine tumors. Int J Radiat Oncol Biol Phys 16: 931-934
17. Trotter MJ, Chaplin DJ and Olive PL (1989b) Use of a carbocyanine dye as a marker of functional vasculature in murine tumours. Br J Cancer 59: 706-709
18. van Geel IPJ, Oppelaar H, Rijken PFJW, Bernsen HJJA, Hagemeier NEM, van der Kogel AJ, Hodgkiss RJ and Stewart FA (1996) Vascular perfusion and hypoxic areas in RIF-1 tumours after photodynamic therapy. Br J Cancer 73: 288-293
19. Vaupel PW (1993) Oxygenation of solid tumors. In Drug Resistance in Oncology. Teicher BA. (ed), pp. 53-85. Marcel Dekker: New York
20. Vecchi A, Garlanda C, Lampugnani MG, Resnati M, Matteucci C, Stoppacciaro A, Schnurch H, Risau W, Ruco L, Mantovani A and Dejana E (1994) Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol 63: 247-254