Establishment of a shear stress protocol to study the mechanosensitivity of human primary osteogenic cells in vitro

Biomedical Sciences Instrumentation

Volumn 35, Issue , 1999, Pages 327-332

  Billotte, William G ^a   Hofmann, Marie Claude ^a  

^a UNIVERSITY OF DAYTON   (United States)

Author keywords

Isolation; Magnetic beads; Osteoprogenitors; Shear stress

Indexed keywords

BONE; CELLS; MATHEMATICAL MODELS; PHYSIOLOGICAL MODELS; SHEAR STRESS; STRESS ANALYSIS;

HUMAN OSTEOGENIC CELLS; OSTEOPROGENITORS;

BIOMECHANICS;

BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL STRAIN 3T3; CONFERENCE PAPER; CONTROLLED STUDY; HUMAN; HUMAN CELL; MECHANICAL STRESS; MESENCHYME; OSTEOBLAST; SHEAR STRESS; STEM CELL; TECHNIQUE;

3T3 CELLS; ALKALINE PHOSPHATASE; ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; HUMANS; MICE; OSTEOBLASTS; OSTEOCALCIN; OSTEOGENESIS; STRESS, MECHANICAL;

EID: 0032961694     PISSN: 00678856     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (6)

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2. Galbraith C.G., Skalak R., and Chien S. (1998). Shear stress induces spatial reorganization of the endothelial cell cytoskeleton. Cell Motil. Cytoskeleton 40: 317-330
3. Ingber D.E. and Folkman J. (1989). Tension and Compression as Basic Determinants of Cell Form and Function: Utilization of a Cellular Tensegrity Mechanism. In: Cell Shape: Determinants, Regulation, and Regulatory Role. Academic Press, New York, pp 3-31
4. Simmons P.J. and Torok-Storb B. (1991). Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78: 55-62.
5. + fraction of adult human bone marrow contains the osteogenic precursors. Blood 84:4164-4173.
6. Narisawa S, Sowadski JM, and Millan JL (1990). An active site mutant of human placental alkaline phosphatase with deficient enzymatic activity and preserved immunoreactivity. Clin Chim Acta 186: 189-196