Cloning and characterization of three genes (SUT1-3) encoding glucose transporters of the yeast Pichia stipitis

Molecular Microbiology

Volumn 31, Issue 3, 1999, Pages 871-883

  Weierstall, Thomas ^a   Hollenberg, Cornelis P ^a   Boles, Eckhard ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; GLUCOSE TRANSPORTER; MONOSACCHARIDE; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME SPECIFICITY; FUNGAL GENETICS; GENE EXPRESSION; GENETIC TRANSCRIPTION; GLUCOSE TRANSPORT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OXYGEN SUPPLY; PHENOTYPE; PICHIA STIPITIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BETA-GALACTOSIDASE; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENOMIC LIBRARY; KINETICS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; PICHIA; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

PICHIA PASTORIS; PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE;

EID: 0032961329     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01224.x     Document Type: Article

References (60)

1. Bairoch, A. (1993) The PROSITE dictionary of sites and patterns in proteins, its current status. Nucleic Acids Res 21: 3097-3103.
2. Billard, P., Menart, S., Blaisonneau, J., Bolotin-Fukuhara, M., Fukuhara, H., and Wesolowski-Louvel, M. (1996) Glucose uptake in Kluyveromyces lactis: role of the HGT1 gene in glucose transport. J Bacteriol 178: 5860-5866.
3. Birnboim, H.C., and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513-1523.
4. Bisson, L.F., and Fraenkel, D.G. (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci USA 80: 1730-1734.
5. Boles, E., and Hollenberg, C.P. (1997) The molecular genetics of hexose transport in yeasts. FEMS Microbiol Rev 21: 85-111.
6. Botstein, D., Falco, S.C., Stewart, S.E., Brennan, M., Scherer, S., Stinchcomb, D.T., Struhl, K., and Davis, R.W. (1979) Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 1: 17-24.
7. Bruinenberg, P.M., De Bot, P.H.M., Van Dijken, J.P., and Scheffers, W.A. (1984) NADH-linked aldose reductase: the key to anaerobic fermentation of xylose by yeasts. Appl Microbiol Biotechnol 19: 256-260.
8. Bucher, P., and Trifonov, E.N. (1986) Compilation and analysis of eukaryotic POL II promoter sequences. Nucleic Acids Res 14: 10009-10025.
9. Cho, J.Y., and Jeffries, T.W. (1998) Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions. Appl Environ Microbiol 64: 1350-1358.
10. Dellweg, H., Rizzi, M., Mether, H., and Debus, D. (1984) Xylose fermentation by yeasts. 3. Comparison of Pachysolen tannophilus and Pichia stipitis. Biotechnol Lett 6: 395-400.
11. Denis, C.L., Ciriacy, M., and Young, E.T. (1981) A positive regulatory gene is required for accumulation of the functional messenger RNA for the glucose repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J Mol Biol 148: 355-368.
12. Does, A.L., and Bisson, L.F. (1989) Characterization of xylose uptake in the yeasts Pichia heedii and Pichia stipitis. Appl Environ Microbiol 55: 159-164.
13. Dohmen, R.J., Strasser, A.W.M., Höner, C.B., and Hollenberg, C.P. (1991) An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7: 691-692.
14. Du Preez, J.C., Bosch, M., and Prior, B.A. (1986) The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis. Appl Microbiol Biotechnol 23: 228-233.
15. Du Preez, J.C., van Driessel, B., and Prior, B.A. (1989) Effect of aerobiosis on fermentation and key enzyme levels during growth of Pichia stipitis, Candida shehatae and Candida tenius on D-xylose. Arch Microbiol 152: 143-147.
16. Feinberg, A.P., and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6-13.
17. Feinberg, A.P., and Vogelstein, B. (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266-267.
18. Goldberg, M. (1979) Sequence Analysis of Drosophila Histone Genes. Stanford, CA: Stanford University Press.
19. Hagedorn, J. (1990) Isolierung und Charakterisierung von Mutanten im Xylosestoffwechsel und Entwicklung eines Transformationssystems für die Hefe Pichia stipitis (PhD Thesis). Düsseldorf: Dusseldorf University Press.
20. Hahn-Hägerdal, B., Jeppsson, H., Skoog, K., and Prior, B.A. (1994) Biochemistry and physiology of xylose fermentation by yeasts. Enzyme Microb Technol 16: 933-943.
21. Hamilton, R., Watanabe, C.K., and de Boer, H.A. (1987) Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic Acids Res 15: 3581-3591.
22. Henikoff, S. (1984) Unidirectinoal digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351-359.
23. Henikoff, S., and Cohen, E.H. (1984) Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae. Mol Cell Biol 4: 1515-1520.
24. Hill, J.E., Myers, A.M., Koerner, T.J., and Tzagoloff, A. (1986) Yeast/E.coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167.
25. Jeffries, T.W. (1985) Emerging technology for fermenting D-xylose. Trends Biochem Sci 3: 208-212.
26. Jeffries, T.W., and Kurtzman, C.P. (1994) Strain selection, taxonomy, and genetics of xylose-fermenting yeasts. Enzyme Microb Tecnnol 16: 922-932.
27. Kilian, S.G., and van Uden, N. (1988) Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis. Appl Microbiol Biotechnol 27: 545-548.
28. Klebe, R.J., Harriss, J.V., Sharp, Z.D., and Douglas, M.G. (1983) A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene 25: 333-341.
29. Kötter, P., and Ciriacy, M. (1993) Xylose fermentation by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 38: 776-783.
30. Kötter, P., Amore, R., Hollenberg, C.P., and Ciriacy, M. (1990) Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant. Curr Genet 18: 493-500.
31. Kozak, M. (1981) Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res 9: 5233-5252.
32. Kruckeberg, A.L. (1996) The hexose transporter family of Saccharomyces cerevisiae. Arch Microbiol 166: 283-292.
33. 13C) xylose metabolism in Pichia stipitis under aerobic and anaerobic conditions. Appl Microbiol Biotechnol 28: 293-296.
34. Lu, P., Davis, B.P., and Jeffries, T.W. (1998) Cloning and characterization of two pyruvate decarboxylase genes from Pichia stipitis CBS 6045. Appl Environ Microbiol 64: 94-97.
35. Marger, M.D., and Saier, M.H. (1993) A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci 18: 13-20.
36. McMaster, G.K., and Carmichael, G.G. (1977) Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels using glyoxal and acridine orange. Proc Natl Acad Sci USA 74: 4835-4838.
37. Metzger, M.H., and Hollenberg, C.P. (1994) Isolation and characterization of the Pichia stipitis transketolase gene and expression in a xylose-utilising Saccharomyces cerevisiae transformant. Appl Microbiol Biotechnol 42: 319-325.
38. Miller, J.H. (1972) Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
39. Mueckler, M., Caruso, C., Baldwin, S.A., Panico, M., Blench, I., Morris, H.R., Allard, W.J., Lienhard, G.E., and Lodish, H.F. (1985) Sequence and structure of a human glucose transporter. Science 229: 941-945.
40. Myers, A.M., Tzagoloff, A., Kinney, D.M., and Lusty, C.J. (1986) Yeast shuttle and integrative vectors with multiple cloning sites for construction of lacZ fusions. Gene 45: 299-310.
41. Passoth, V., Schäfer, B., Liebel, B., Weierstall, T., and Klinner, U. (1998) Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia stipitis and identification of the fermentative ADH. Yeast 14: 1311-1325.
42. Ramos, J., Szkutnicka, K., and Cirillo, V.P. (1989) Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles. J Bacteriol 171: 3539-3544.
43. Reifenberger, E., Freidel, K., and Ciriacy, M. (1995) Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol Microbiol 16: 157-167.
44. Reifenberger, E., Boles, E., and Ciriacy, M. (1997) Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repression. Eur J Biochem 245: 324-333.
45. Robzyk, K., and Kassir, Y. (1992) A simple and highly efficient procedure for rescuing autonomous plasmids from yeast. Nucleic Acids Res 20: 3790.
46. Rosenberg, S.L. (1980) Fermentation of pentose sugars to ethanol and other neutral products by microorganisms. Enzyme Microbiol Technol 2: 185-193.
47. Rothstein, R. (1991) Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 194: 281-301.
48. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
49. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
50. Sarwald, C. (1989) Konstruktion einer genomischen Genbank der Hefe Pichia stipitis und Isolation verschiedener Aminosäure-und Nukleotid-Synthesegene durch Komplementation von Saccharomyces cerevisiae-Mutanten (Ph.D. Thesis). Düsseldorf: Dusseldorf University Press.
51. Schneider, H. (1989) Conversion of pentoses to ethanol by yeasts and fungi. CRC Crit Rev Biochem Mol Biol 9: 1-40.
52. Sherman, F., Fink, G.R., and Hicks, J.B. (1983) Methods in Yeast Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
53. Slininger, P.J., Bothast, R.J., Okos, M.R., and Ladisch, M.R. (1985) Comparative evaluation of ethanol production by xylose-fermenting yeasts presented high xylose concentrations. Biotechnol Lett 7: 431-436.
54. Toivola, A., Yarrow, D., Van Den Bosch, E., Van Dijken, J.P., and Scheffers, W.A. (1984) Alcoholic fermentation of D-xylose by yeasts. Appl Environ Microbiol 47: 1221-1223.
55. Van Urk, H., Postma, E., Scheffers, W.A., and Van Dijken, J.P. (1989) Glucose transport in Crabtree-positive and Crabtree-negative yeasts. J Gen Microbiol 135: 2399-2406.
56. Walsh, M.C., Smits, H.P., Scholte, M., and Van Dam, K. (1994) Affinity of glucose transport in Saccharomyces cerevisiae is modulated during growth on glucose. J Bacteriol 176: 953-958.
57. Wesolowski-Louvel, M., Goffrini, P., Ferrero, I., and Fukuhara, H. (1992) Glucose transport in the yeast Kluyveromyces lactis. I. Properties of an inducible low-affinity glucose transporter gene. Mol Gen Genet 233: 89-96.
58. Wyman, C.E., and Goodman, B.J. (1993) Biotechnology for production of fuels, chemicals, and materials from biomass. Appl Biochem Biotechnol 39/40: 41-59.
59. Zamenhoff, S. (1957) Preparation and assay of desoxyribonucleic acids from animal tissue. Methods Enymol 3: 696-704.
60. Zaret, K.S., and Sherman, F. (1982) DNA sequence required for efficient transcription termination in yeast. Cell 28: 563-573.