PCR-mediated recombination: A general method applied to construct chimeric infectious molecular clones of plasma-derived HIV-1 RNA

Nature Medicine

Volumn 5, Issue 2, 1999, Pages 239-242

  Fang, Guowei ^a   Weiser, Barbara ^a,b   Visosky, Aloise ^a   Moran, Timothy ^a   Burger, Harold ^a,b  

^a WADSWORTH CENTER   (United States)

^b ALBANY MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

BIOTECHNOLOGY; CHIMERA; DNA SEQUENCE; GENETIC RECOMBINATION; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHORT SURVEY; VIRUS INFECTIVITY;

CHIMERA; CLONING, MOLECULAR; DNA, VIRAL; GENETIC ENGINEERING; HIV-1; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RESTRICTION MAPPING; RNA, VIRAL; SEQUENCE ANALYSIS, DNA;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES; SIMIAN IMMUNODEFICIENCY VIRUS;

EID: 0032957538     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/5607     Document Type: Short Survey

References (20)

1. Saiki, R.K. et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491 (1988).
2. Meyerhans, A., Vartanian, J.P. & Wain-Hobson, S. DNA recombination during PCR. Nucl. Acids Res. 18, 1687-1691 (1990).
3. Horton, R.M., Hunt, H.D., Ho, S.N., Pullen, J. & Pease, L.R. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension. Gene 77, 61-68 (1989).
4. Horton, R.M., Cai, Z.L., Ho, S.N. & Pease, L.R. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction. Biotechniques 8, 528-535 (1990).
5. Yolov, A.A. & Shabarova, Z.A. Constructing DNA by polymerase recombination. Nucl. Acids Res. 18, 3483-3486 (1990).
6. Klug, J., Wolf, M. & Beato, M. Creating chimeric molecules by PCR directed homologous DNA recombination. Nucl. Acids Res. 19, 2793 (1991).
7. Gu, H., Planas, J., Gomez, R. & Wilson, D.J. Full length mouse glycoghorin gene constructed using recombinant polymerase chain reaction. Biochem. Biophys. Res Commun. 177, 202-208 (1991).
8. Sandhu, G.S., Aleff, R.A. & Kline, B.C. Dual asymmetric PCR: One-step construction of synthetic genes. Biotechniques 12, 14-16 (1992).
9. Eckert, K.A. & Kunkel, T.A. DNA polymerase fidelity and the polymerase chain reaction. PCR Meth. Applic. 1, 17-24 (1991).
10. Mattila, P., Korpela, J., Tenkanen, T. & Pitkanen, K. Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase - an extremely heat-stable enzyme with proofreading activity. Nucl. Acids Res. 19, 4967-4973 (1991).
11. Coffin, J.M. HIV population dynamics in vivo: Implications for genetic variation, pathogenesis, and therapy. Science 267, 483-489 (1995).
12. Chun, T.W. et al. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature 387, 183-188 (1997).
13. Fang, G., Weiser, B., Visosky, A., Townsend, L. & Burger, H. Molecular cloning of full-length HIV-1 genomes directly from plasma viral RNA. J. AIDS 12, 352-357 (1996).
14. Fang, G. et al. Maternal plasma human immunodeficiency virus type 1 RNA level: A determinant and projected threshold for mother-to-child transmission. Proc. Natl. Acad. Sci. USA 92, 12100-12104 (1995).
15. Human Retroviruses and AIDS (eds. Myers, G. et al.) (Los Alamos National Laboratory, Los Alamos, New Mexico, 1996).
16. Salminen, M.O. et al. Recovery of virtually full-length HIV-1 provirus of diverse subtypes from primary virus cultures using the polymerase chain reaction. Virology 213, 80-86 (1995).
17. Lundberg, K.S et al. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene 108, 1-6 (1991).
18. Flaman, J.M. et al. A rapid PCR fidelity assay. Nucl. Acids Res. 22, 3259-3260 (1994).
19. Piatak, M., Jr. et al. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science 259, 1749-1 754 (1993).
20. Horton, R.M. SOEing together tailor-made genes. Mol. Biotechnol. 3, 93-99 (1995).