Repression at a distance by the global regulator KorB of promiscuous IncP plasmids

Molecular Microbiology

Volumn 32, Issue 3, 1999, Pages 519-532

  Jagura Burdzy, Grazyna ^a,b   Macartney, Donia P ^a   Zatyka, Malgorzata ^a,b   Cunliffe, Lesley ^a   Cooke, Dunstan ^a,c   Huggins, Cerys ^a,d   Westblade, Lars ^a   Khanim, Farhat ^a,e   Thomas, Christopher M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY OF READING   (United Kingdom)

^e KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DEOXYRIBONUCLEASE I; RNA POLYMERASE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA FOOTPRINTING; GENE DELETION; GENE REPRESSION; MUTAGENESIS; NONHUMAN; OPERATOR GENE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; OPERATOR REGIONS (GENETICS); PLASMIDS; PROMOTER REGIONS (GENETICS); REPRESSION; REPRESSOR PROTEINS; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0032956390     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01365.x     Document Type: Article

References (45)

1. Arnold, F.H. (1991) Metal affinity separations - a new dimension in protein processing. Bio/Technology 9: 151-156.
2. Balzer, D., Ziegelin, G., Pansegrau, W., Kruft, V., and Lanka, E. (1992) KorB protein of promiscuous plasmid RP4 recognises inverted sequence repetitions in regions essential for conjugative transfer. Nucleic Acids Res 20: 1851-1858.
3. Birnboim, H.C., and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513-1523.
4. Calladine, C.R., and Drew, H.R. (1997) Understanding DNA. San Diego: Academic Press.
5. Collado-Vides, J., Magasanik, B., and Gralla, J.D. (1991) Control site location and transcriptional activity in Escherichia coli. Microb Rev 55: 371-394.
6. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395.
7. Gordon, G.S., Sitnikow, D., Webb, C.D., Teleman, A., Straight, A., Losick, R., et al. (1997) Chromosome and low copy plasmid segregation in E. coli: visual evidence for distinct mechanisms. Cell 90: 1113-1121.
8. Gornall, A.G., Bardawill, C.J., and David, M.M. (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177: 751-766.
9. Hanai, R., Ruiping, L., Benedetti, P., Caron, P.R., Lynch, A.S., and Wang, J.C. (1996) Molecular dissection of a protein SopB essential for Escherichia coli F plasmid partition. J Biol Chem 271: 17469-17475.
10. Jagura-Burdzy, G., and Thomas, C.M. (1992) kfrA gene of broad host range plasmid RK2 encodes a novel DNA binding protein. J Mol Biol 225: 651-660.
11. Jagura-Burdzy, G., and Thomas, C.M. (1994) KorA protein of promiscuous plasmid RK2 controls a transcriptional switch between divergent operons for plasmid replication and conjugative transfer. Proc Natl Acad Sci USA 91: 10571-10575.
12. Jagura-Burdzy, G., and Thomas, C.M. (1995) Purification of KorA protein from broad-host-range plasmid RK2: definition of a hierarchy of KorA operators. J Mol Biol 253: 39-50.
13. Jagura-Burdzy, G., and Thomas, C.M. (1997) Dissection of the switch between genes for replication and transfer of promiscuous plasmid RK2: basis of the dominance of trfAp over trbAp and specificity for KorA in controlling the switch. J Mol Biol 265: 507-518.
14. Jagura-Burdzy, G., Ibbotson, J.P., and Thomas, C.M. (1991) The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity. J Bacteriol 173: 826-833.
15. Jagura-Burdzy, G., Khanim, F., Smith, C.A., and Thomas, C.M. (1992) Cross-talk between plasmid vegetative replication and conjugative transfer: regulation of the trfA operon by trbA of broad-host-range plasmid RK2. Nucleic Acids Res 20: 3939-3944.
16. Kahn, M., Kolter, R., Thomas, C.M., Figurski, D., Meyer, R., Remaut, E., et al. (1979) Plasmid cloning vehicles derived from ColE1, F, R6K and RK2. Methods Enzymol 68: 268-280.
17. Kornacki, J.A., Balderes, P.J., and Figurski, D.H. (1987) Nucleotide sequence of korB, a replication control gene of broad host range plasmid RK2. J Mol Biol 198: 211-222.
18. Lobocka, M., and Yarmolinsky, M. (1996) P1 plasmid partition - a mutational analysis of ParB. J Mol Biol 259: 366-382.
19. Macartney, D.P., Williams, D.R., Stafford, T., and Thomas, C.M. (1997) Divergence and conservation of the partitioning and global regulation functions in the central control region of the IncP plasmids RK2 and R751. Microbiology 143: 2167-2177.
20. Mohl, D.A., and Gober, J.W. (1997) Cell cycle-dependent polar localization of chromosome partitioning proteins in Caulobacter crescentus. Cell 88: 675-684.
21. Motallebi-Veshareh, M., Rouch, D., and Thomas, C.M. (1990) A family of ATPases involved in the active partitioning of diverse bacterial plasmids. Mol Microbiol 4: 1455-1463.
22. Motallebi-Veshareh, M., Balzer, D., Lanka, E., Jagura-Burdzy, G., and Thomas, C.M. (1992) Conjugative transfer functions of broad host range plasmid RK2 are coregulated with vegetative replication. Mol Microbiol 6: 607-920.
23. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., and Erlich, H. (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 51: 263-273.
24. Niki, H., and Hiraga, S. (1997) Subcellular distribution of actively partitioning F plasmid during the cell division cycle of E. coli. Cell 90: 951-957.
25. Pansegrau, W., Lanka, E., Barth, P.T., Figurski, D.H., Guiney, D.G., Haas, D., et al. (1994) Complete nucleotide sequence of Birmingham IncPα plasmids: compilation and comparative analysis. J Mol Biol 239: 623-663.
26. Perez-Martin, J., de Rojo, F., and Lorenzo, V. (1994) Promoters responsive to DNA bending: a common theme in prokaryotic gene expression. Microbiol Rev 58: 268-290.
27. Radnedge, L., Davis, M.A., and Austin, S.J. (1996) P1 and P7 plasmid partition - ParB protein bound to its partition site makes a separate discriminator contact with the DNA that determines species specificity. EMBO J 15: 1155-1162.
28. Sambrook, J., Fritsch, E.F., and Maniatis, T.M. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
29. Sancar, A., Hack, A.M., and Rupp, D. (1979) Simple method for identification of plasmid-encoded proteins. J Bacteriol 137: 692-693.
30. Sharpe, M., and Errington, J. (1996) The Bacillus subtilis soj-spoJ locus is required for centromere-like function involved in prespore chromosome partitioning. Mol Microbiol 21: 501-505-9.
31. Shingler, V., and Thomas, C.M. (1984a) Analysis of the trfA region of broad host range plasmid RK2 by transposon mutagenesis and identification of polypeptide products. J Mol Biol 175: 229-250.
32. Shingler, V., and Thomas, C.M. (1984b) Transcription in the trfA region of broad host range plasmid RK2 is regulated by trfB and korB. Mol Gen Genet 195: 523-529.
33. Studier, F.W., and Moffatt, B.A. (1986) Use of bacteriophage-T7 RNA polymerase to direct selective high level expression of cloned genes. J Mol Biol 189: 113-130.
34. Theophilus, B.D.M., and Thomas, C.M. (1987) Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad-host-range plasmid RK2. Nucleic Acids Res 15: 7443-7450.
35. Thomson, V.J., Jovanovic, O.S., Pohlman, R.F., and Chang, C.-H., and Figurski, D.H. (1993) Structure, function and regulation of the kilB locus of promiscuous plasmid RK2. J Bacteriol 175: 2423-2435.
36. Thorsted, P.B., Shah, D.S., Macartney, D.P., and Kostelidou, K., and Thomas, C.M. (1996) Conservation of the genetic switch between replication and transfer genes of IncP plasmids but divergence of the replication functions which are major host range determinants. Plasmid 36: 95-111.
37. Thorsted, P.B., Macartney, D.P., Akhtar, P., Haines, A.S., Ali, N., Davidson, P., et al. (1998) Complete sequence of the IncPβ plasmid R751: implications for the evolution and organisation of the IncP backbone. J Mol Biol 282: 696-990.
38. Wake, R.G., and Errington, J. (1995) Chromosome partitioning in bacteria. Annu Rev Genet 29: 41-67.
39. Webb, C.D., Teleman, A., Gordon, S., Straight, A., Belmont, A., Lin, D.C.-H., et al. (1997) Bipolar localisation of the replication origins of chromosomes in vegetative and sporulating cells of B. subtilis. Cell 88: 667-674.
40. Williams, D.R., and Thomas, C.M. (1992) Active partitioning of bacterial plasmids. J Gen Microbiol 138: 1-16.
41. Williams, D.R., Motallebi-Vershareh, M., and Thomas, C.M. (1993) Multifunctional repressor KorB can block transcription by preventing isomerization of RNA polymerase-promoter complexes. Nucleic Acids Res 21: 1141-1148.
42. B3. Microbiology 144: 3369-3378.
43. Zatyka, M., Jagura-Burdzy, G., and Thomas, C.M. (1994) Regulation of transfer genes of promiscuous IncPα plasmid RK2: repression of Tra1 region transcription both by relaxosome proteins and by the Tra2 regulator TrbA. Microbiology 140: 2981-2990.
44. Zatyka, M., Jagura-Burdzy, G., and Thomas, C.M. (1997) Transcriptional and translational control of the genes for the mating pair formation apparatus of promiscuous IncP plasmids. J Bacteriol 179: 7201-7209.
45. Zukowski, M.M., Gaffney, D.F., Speck, D., Kauffman, M., Findeli, A., Wisecup, A., et al. (1983) Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonad gene. Proc Natl Acad Sci USA 80: 1101-1105.