The pecT repressor coregulates synthesis of exopolysaccharides and virulence factors in Erwinia chrysanthemi

Molecular Plant-Microbe Interactions

Volumn 12, Issue 1, 1999, Pages 45-52

  Condemine, Guy ^a   Castillo, Arnaud ^a   Passeri, Fabrice ^a   Enard, Corine ^b  

^a CNRS   (France)

^b AGROPARISTECH   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EXOPOLYSACCHARIDE; OPERON; PATHOGENICITY; PROMOTER REGION; VIRULENCE;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CHRYSANTHEMI; ERWINIA CHRYSANTHEMI STR. 3937; SAINTPAULIA IONANTHA;

EID: 0032955896     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI.1999.12.1.45     Document Type: Article

References (47)

1. Arakawa, Y., Wacharotayankun, R., Nagatsuka, T., Ito, H., Kato, N., and Ohta, M. 1995. Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J. Bacteriol. 177:1788-1796.
2. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. 1987. Current Protocols in Molecular Biology. Wiley-Interscience, New York.
3. Bailey, M. J. A., Hughes, C., and Koronakis, V. 1997. RfaH and the ops element, components of a novel system controlling bacterial transcription elongation. Mol. Microbiol. 26:845-851.
4. Bardonnet, N., and Blanco, C. 1992. uidA antibiotic resistance cassettes for insertion mutagenesis, gene fusion and genetic constructions. FEMS Microbiol. Lett. 93:243-248.
5. Beaulieu, C., and Van Gijsegem, F. 1990. Identification of plant-inducible genes in Erwinia chrysanthemi 3937. J. Bacteriol. 172: 1569-1575.
6. Beck von Bodman, S., and Farrand, S. K. 1995. Capsular polysaccharide biosynthesis and pathogenicity in Erwinia stewartii require induction by an N-acylhomoserine lactone autoinducer. J. Bacteriol. 177:5000-5008.
7. Beck von Bodman, S., Majerczak, D. R., and Coplin, D. L. 1998. A negative regulator mediates quorum-sensing control of exopolysaccharide production in Pantoea stewartii subsp. stewartii. Proc. Natl. Acad. Sci. USA 95:7687-7692.
8. Brumbley, S. M., Carney, B. F., and Denny, T. P. 1993. Phenotype conversion in Pseudomonas solanacearum due to spontaneous inactivation of PhcA, a putative LysR transcriptional regulator. J. Bacteriol. 175:5477-5487.
9. Bugert, P., and Geider, K. 1995. Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol. Microbiol. 15:917-933.
10. Castillo, A., and Reverchon, S. 1997. Characterization of the pecT control region from Erwinia chrysanthemi 3937. J. Bacteriol. 179:4909-4918.
11. Clough, S. J., Lee, K.-E., Schell, M. A., and Denny, T. P. 1997. A two-component system in Ralstonia (Pseudomonas) solanacearum modulates production of a PhcA-regulated virulence factor in response to 3-hydroxypalmitic acid methyl ester. J. Bacteriol. 179:3639-3948.
12. Condemine, G., and Robert-Baudouy, J. 1987a. 2-keto-3-deoxygluconate transport system in Erwinia chrysanthemi. J. Bacteriol. 169: 1972-1978.
13. Condemine, G., and Robert-Baudouy, J. 1987b. Tn5 insertion in kdgR, a regulatory gene of the polygalacturonate pathway in Erwinia chrysanthemi. FEMS Microbiol. Lett. 42:39-46.
14. Denny, T. P. 1995. Involvement of bacterial polysaccharides in plant pathogenesis. Annu. Rev. Phytopathol. 33:173-197.
15. Denny, T. P., and Baek, S.-R. 1991. Genetic evidence that extracellular polysaccharide is a virulence factor of Pseudomonas solanacearum. Mol. Plant-Microbe Interact. 4:198-206.
16. Expert, D., and Toussaint, A. 1985. Bacteriocin-resistant mutants of Erwinia chrysanthemi: Possible involvement of iron acquisition in phytopathogenicity. J. Bacteriol. 163:221-227.
17. Gottesman, S. 1995. Regulation of capsule synthesis: Modification of a two component paradigm by an accessory unstable protein. Pages 253-262 in: Two-Component Signal Transduction. J. A. Hoch and T. J. Silhavy, eds. American Society for Microbiology, Washington, DC.
18. Grangeasse, C., Doublet, P., Vaganay, E., Vincent, C., Deléage, G., Duclos, B., and Cozzone, A. J. 1997. Characterization of a bacterial gene encoding an autophosphorylating protein tyrosine kinase. Gene 204: 259-265.
19. Hobbs, M., and Reeves, P. R. 1994. The JUMPstart sequence: A 39 bp element common to several polysaccharide gene clusters. Mol. Microbiol. 12:855-856.
20. Huang, J., Carney, B. F., Denny, T. P., Weissinger, A. K., and Schell, M. A. 1995. A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum. J. Bacteriol. 177: 1259-1267.
21. Hugouvieux-Cotte-Pattat, N., Reverchon, S., and Robert-Baudouy, J. 1989. Expanded linkage map of Erwinia chrysanthemi strain 3937. Mol. Microbiol. 3:573-581.
22. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. 1985. Lactose catabolism in Erwinia chrysanthemi. J. Bacteriol. 162:248-255.
23. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. 1989. Isolation of Erwinia chrysanthemi mutants altered in pectinolytic enzyme production. Mol. Microbiol. 3:1587-1597.
24. Jiang, X.-M., Neal, B., Santiago, F., Lee, S. J., Romana, L. K., and Reeves, P. R. 1991. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol. Microbiol. 5:695-713.
25. Kolb, A., Busby, S., Buc, H., Garges, S., and Adhya, S. 1993. Transcriptional regulation by cAMP and its receptor protein. Annu. Rev. Biochem. 62:749-795.
26. Köplin, R., Wang, G., Hötte, B., Priefer, U. B., and Pühler, A. 1993. A 3.9-kb DNA region of Xanthomonas campestris pv. campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose. J. Bacteriol. 175:7786-7792.
27. Leigh, J. A., and Coplin, D. L. 1992. Exopolysaccharides in plant-bacterial interactions. Annu. Rev. Microbiol. 46:307-346.
28. Marolda, C. L., and Valvano, M. A. 1995. Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: Identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rrfE gene. J. Bacteriol. 177:5539-5546.
29. Miller, J. H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
30. Missiakas, D., and Raina, S. 1997. Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: Role of two new phosphoprotein phosphatases PrpA and PrpB. EMBO J. 16:1670-1685.
31. Moran, F., and Starr, M. P. 1969. Metabolic regulation of polygalacturonic acid trans-eliminase in Erwinia. Eur. J. Biochem. 11:291-295.
32. Nasser, W., Reverchon, S., Condemine, G., and Robert-Baudouy, J. 1994. Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis. J. Mol. Biol. 236:427-440.
33. Novel, G., Didier-Fichet, M. L., and Stoeber, F. 1974. Inductibility of β-glucuronidase in wild-type and hexuronate-negative mutants of Escherichia coli K-12. J. Bacteriol. 120:89-95.
34. Reeves, P. R., Hobbs, M., Valvano, M. A., Skurnik, M., Whitfield, C., Coplin, D., Kido, N., Klena, J., Maskell, D., Raetz, C. R. H., and Rick, P. D. 1996. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol. 4:495-503.
35. Resibois, A., Colet, M., Faelen, M., Schoonejans, E., and Toussaint, A. 1984. PhiEC2, a new generalised transducing phage of Erwinia chrysanthemi. Virology 137:102-112.
36. Reverchon, S., Expert, D., Robert-Baudouy, J., and Nasser, W. 1997. The cyclic AMP receptor protein is the main activator of the pectinolysis genes in Erwinia chrysanthemi. J. Bacteriol. 179:3500-3508.
37. Reverchon, S., Nasser, W., and Robert-Baudouy, J. 1994. pecS: A locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi. Mol. Microbiol. 11:1127-1139.
38. Roeder, D. L., and Collmer, A. 1985. Marker-exchange mutagenesis of pectate lyase isozyme gene in Erwinia chrysanthemi. J. Bacteriol. 164:51-56.
39. Sambrook, J., Fritsch, E. F., and Maniatis, T. A. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
40. Shevchik, V. E., and Hugouvieux-Cotte-Pattat, N. 1997. Identification of a bacterial pectin acetyl esterase in Erwinia chrysanthemi. Mol. Microbiol. 24:1285-1301.
41. Shevchik, V. E., Robert-Baudouy, J., and Hugouvieux-Cotte-Pattat, N. 1997. The pectate lyase PelI of Erwinia chrysanthemi belongs to a new family. J. Bacteriol. 179:7321-7330.
42. Simon, R., Priefer, U., and Pühler, A. 1983. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1:784-791.
43. Simon, R., Quandt, J., and Klipp, W. 1989. New derivatives of transposon Tn5 suitable for mobilisation of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80: 161-169.
44. Surgey, N., Robert-Baudouy, J., and Condemine, G. 1996. The Erwinia chrysanthemi pecT gene regulates pectinase gene expression. J. Bacteriol. 178:1593-1599.
45. Wacharotayankun, R., Arakawa, Y., Ohta, M., Hasegawa, T., Mori, M., Horii, T., and Kato, N. 1992. Involvement of rcsB in Klebsiella K2 capsule synthesis in Escherichia coli K-12. J. Bacteriol. 174:1063-1067.
46. Yang, B. Y., Gray, J. S., and Montgomery, R. 1994. Extracellular polysaccharide of Erwinia chrysanthemi Ech6. Int. J. Biol. Macromol. 16: 306-312.
47. Yang, B. Y., Gray, J. S., and Montgomery, R. 1996. Comparison of extracellular polysaccharides of Erwinia chrysanthemi spp. Int. J. Biol. Macromol. 19:223-226.