Localisation of enzymes in live spermatozoa by CellProbe(TM) reagents (preliminary results)

Andrologia

Volumn 31, Issue 1, 1999, Pages 37-42

  Glander, H J ^b   Schaller, J ^a  

^a St Barbara Hospital   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Fluorescence microscopy; Fluorogenic enzyme substrates; Human spermatozoa; Proteases

Indexed keywords

BETA GLUCURONIDASE; CATHEPSIN D; CELL ENZYME; DIPEPTIDYL PEPTIDASE IV; GLUCOSIDASE; GLUTAMYL AMINOPEPTIDASE; REAGENT; SUBTILISIN;

ACROSOME; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ASSAY; ENZYME LOCALIZATION; HUMAN; HUMAN CELL; MALE; SPERMATOZOON;

EVALUATION STUDIES; HUMANS; INDICATORS AND REAGENTS; MALE; MICROSCOPY, FLUORESCENCE; SIGNAL PROCESSING, COMPUTER-ASSISTED; SPERMATOZOA;

EID: 0032954424     PISSN: 03034569     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1439-0272.1999.00237.x     Document Type: Article

References (19)

1. Adham IM, Nayernia K, Engel W (1997) Spermatozoa lacking acrosin protein show delayed fertilization. Mol Reprod Dev 46:370-376.
2. Aitken RJ, Paterson M, Fisher H, Buckingham DW, van Duin M (1995) Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function. J Cell Sci 108:2017-2025.
3. Aitken RJ, Buckingham DW, Carreras A, Irvine DS (1996) Superoxide dismutase in human sperm suspensions: relationship with cellular composition, oxidative stress, and sperm function. Free Radic Biol Med 21:495-504.
4. Alkan I, Simsek F, Haklar G, Kervancioglu E, Özveri H, Yalcin S, Akdas A (1997) Reactive oxygen species production by the spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants. J Urol 157:140-143.
5. Barbieri MA, Veisage ML, Paolicchi F., Fornes MW, Sosa MA, Mayorga LS, Bustos-Obregón E, Bertini F (1996) Affinity sites for β-glucuronidase on the surface of human spermatozoa. Andrologia 28:327-333.
6. Carter J (1996) Live cell functional enzymology using Cell Probe™ reagents. Presentation at the XVIII Congress of the International Society for Analytical Cytology, Proc Congr, Rimini, Italy, p. 1.
7. Gavella M, Lipovac V, Vucic M, Rocic B (1996) Relationship of sperm superoxide dismutase-like activity with other sperm-specific enzymes and experimentally induced lipid peroxidation in infertile men. Andrologia 28:223-229.
8. Geva E, Bartoov B, Zabludovsky N, Lessing JB, Lernev-Geva L, Amit A (1996) The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program. Fertil Steril 66:430-434.
9. Griveau JF, LeLannou D (1997) Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl 20:61-69.
10. Hamilton M, Steele B (1995) The use of novel fluorogenic enzyme substrates to detect nucleated red blood cells. Clin Chem 41:S239.
11. Kiefer MC, Tucker JE, Joh R, Landsberg KE, Satiman D, Barr PJ (1991) Identification of a second human subtilisin-like protease gene in the fes/fps region of chromosome 15. DANN Cell Biol 10:757-769.
12. Klemm U, Mulller-Esterl W, Engel W (1991) Acrosin, the peculiar sperm-specific serine proteinase. Hum Genet 87:635-641.
13. Lucas F, Kuylen N, Galiounghi A, Barcelon M, Carter J (1996) New method for identification of nucleated red blood cells in a lysed leukocyte cell preparation by flow cytometry. Labor Hematol 2:68.
14. Rothe G, Klingel S, Assfalg-Machleidt I, Machleidt W, Zirkelbach C, Banati RB, Mangel WF, Valet G (1992) Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe-Seyler 373:547-554.
15. Schill WB (1990) Determination of active, non-zymogen acrosin, proacrosin, and totalacrosin in different andrological patients. Arch Dermatol Res 282:335-342.
16. Töpfer-Petersen E, Calvete JJ, Sanz L, Sinowatz F (1995) Carbohydrate-and heparin-binding proteins in mammalian fertilization. Andrologia 27:303-324.
17. de Vries ACJ, Colenbrander B (1990) Isolation and characlerization of boar spermatozoa with and without a cytoplasmatic droplet. Int J Biochem 22:519-524.
18. World Health Organization (1992) WHO laboratory manual for the examination of human semen and sperm-cervical mucus-interaction, 3rd edn. Cambridge University Press, Cambridge.
19. Zaneveld LDJ, de Jonge CJ (1991) Mammalian sperm acrosomal enzymes and the acrosome reaction. In: A comparative overview of mammalian fertilization. Dunbar BS, O'Rand MG (eds) Plenum press, New York 63-79.