Maximization of poly-β-hydroxybutyrate accumulation by potassium limitation in Methylobacterium organophilum and its related metabolic analysis

Journal of Microbiology and Biotechnology

Volumn 9, Issue 2, 1999, Pages 140-146

  Kim, Seon Won ^a   Kim, Pil ^a   Kim, Jung Hoe ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Intracellular ATP level; Methylobacterium organophilum; Poly hydroxybutyrate; Potassium limitation; Surplus energy

Indexed keywords

2,4 DINITROPHENOL; ADENOSINE TRIPHOSPHATE; AMMONIA; FLAVINE ADENINE NUCLEOTIDE; NITROGEN; POLY(3 HYDROXYBUTYRIC ACID); POTASSIUM; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SULFATE;

ARTICLE; BACTERIAL METABOLISM; BIOACCUMULATION; CELL ENERGY; CONTROLLED STUDY; ENERGY BALANCE; METHYLOBACTERIUM ORGANOPHILUM; NONHUMAN; STOICHIOMETRY;

METHYLOBACTERIUM ORGANOPHILUM;

EID: 0032954135     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Anderson, A. J. and E. A. Dawes. 1990. Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microb. Rev. 54: 450-472.
2. Anthony, C. 1982. The Biochemistry of Methylotrophs. Academic Press, London.
3. Anthony, C. 1986. Bacterial oxidation of methane and methanol. Adv. Microb. Physiol. 27: 113-210.
4. Brandl, H., R. A. Gross, R. W. Lenz, and R. C. Fuller. 1990. Plastics from bacteria and for bacteria: Poly(β-hydroxyalkanoates) as natural, biocompatable, and biodegradable polyester. Adv. Biochem. Eng./Biotech. 41: 77-95.
5. Braunegg, G. and B. Bogensberger. 1985. Growth kinetics and the storage of poly-(-)-3-hydroxybutyric acid in Alcaligenes latus. Acta Biotechnol. 5: 339-345.
6. Braunegg, G., B. Sonnleitner, and R. M. Lafferty. 1978. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. 6: 29-37.
7. Buurman, E. T., J. Pennock, D. W. Tempest, M. J. J. Mattos, and O. M. Neijssel. 1989. Replacement of potassium ions by ammonium ions in different microorganisms grown in potassium-limited chemostat culture. Arch. Microbiol. 152: 58-63.
8. Byrom, D. 1987. Polymer synthesis by microorganisms: Technology and economics. Trends Biotechnol. 5: 246-250.
9. Chistoserdova, L. V. and M. E. Lidstrom. 1994. Genetics of the serine cycle in Methylobacterium extorquens AMI: Identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA. J. Bacteriol. 176: 1957-1968.
10. Daniel, M., J. H. Choi, J. H. Kim, and J. M. Lebeault. 1992. Effect of nutrient deficiency on accumulation and relative molecular weight of poly-β-hydroxybutyric acid by methylotropnic bacterium, Pseudomonas 135. Appl. Microbiol. Biotechnol. 37: 702-706.
11. Doi, Y, Y. Kawaguchi, Y. Nakamura, and M. Kunioka. 1989 Nuclear magnetic resonance studies of poly(3-hydroxybutyrate) and polyphosphate metabolism in Alcaligenes eutrophus. Appl. Env. Microbiol. 55: 2932-2938.
12. Evans, C. G. T., D. Herbert, and D. W. Tempest. 1970. The continuous cultivation of microorganisms 2. Construction of a chemostat, pp. 277-327. In Peterson, P. K. (eds.), Methods in Microbiology. Academic Press, London.
13. Harris D. A. and C. L. Bashford. 1987. Spectrophotometry and Spectrofluorometry, pp. 72-77. IRL Press, Washington DC, U.S.A.
14. Howells, E. R. 1982. Opportunities in biotechnology for the chemical industry. Chem. Ind. 8: 508-511.
15. Kim, B. S., S. Y Lee, and H. N. Chang. 1992. Production of Poly-β-hydroxybutyrate by fed batch culture of recombinant E.coli. Biotechnol. Lett. 14: 811-816.
16. Kim, S. W., P. Kim, H. S. Lee, and J. H. Kim. 1996. High production of poly-β-hydroxybutyrate from Methylobacterium organophilum under potassium limitation. Biotechnol. Lett. 18: 25-30.
17. Linton, J. D. 1990. The relationship between metabolite production and the growth efficiency of the producing organism. FEMS Microbiol. Rev. 75: 1-18.
18. Ludin, A. and A. Thore. 1975. Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP. Analytical Biochem. 66: 47-63.
19. Neijssel, O. M. 1977. The effect of 2,4-dinitrophenol on the growth of Klebsiella aerogenes NCTC 418 in aerobic chemostat culture. FEMS Microbiol. Lett. 1: 47-50.
20. Neijssel, O. M. and. D. W. Tempest. 1987. Relationships Between Nutrient Status of the Environment and Metabolic Fluxes in Micro-organisms, pp. 29-42. Dechema-Monographs vol. 105, VCH Verlagsgesellschaft.
21. OConnor, M. L. and R. S. Hanson. 1977. Enzyme regulation in Methylobacterium organophilum. J. Gen. Microbiol. 101: 327-332.
22. Page, W. J. 1989. Hyperproduction of poly-β-hydroxybutyrate during exponential growth of Azotobacter vinelandii UWD. Appl. Env. Microbiol. 55: 1334-1339.
23. Powell, K. A. and B. A. Collinson. 1980. Microbiological process for the production of poly (β-hydroxybutyric acid). United States Patent Appl. No. 123,354
24. Senior, P. J., G. A. Beech, G. A. F. Richie, and E. A. Dawes. 1972. The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. Biochem. J. 125: 55-66.
25. Slater, E. C. 1967. Application of inhibitors and uncouplers for the study of oxidative phosphorylation, pp. 48-57. In Methods in Enzymology, vol. 10. Academic press, New York, U.S.A.
26. Steinbüchel, A. and H. G. Schlegel. 1989. Excretion of pyruvate by mutants of Alcaligenes eutrophus, which are impaired in the accumulation of poly(β-hydroxybutyric acid) (PHB), under conditions permitting synthesis of PHB. Appl. Microbiol. Biotechnol. 31: 168-175.
27. Steinbüchel, A. 1991. Polyhydroxyalkanoic acids, pp. 123-213. In D. Byrom (eds.), Biomaterials. Macmillan, New York, U.S.A.
28. Stouthamer, A. H. and C. W. Bettenhaussen. 1980. Growth and physiology of potassium-limited chemostat cultures of Paracoccus denitrificans. Arch. Microbiol. 125: 239-244.
29. Suzuki, T., T. Yamane, and S. Shimizu. 1986. Mass production of poly-β-hydroxybutyric acid by fully automatic fed-batch culture of methylotroph. Appl. Microbiol. Biotechnol. 23: 322-329.
30. Tempest, D. W. and O. M. Neijssel. 1981. Metabolic compromises involved in the growth of microorganisms in nutrient-limited (chemostat) environments, pp. 335-356. In A. Hollaender (ed.), Trends in the Biology of Fermentations for Fuels and Chemicals, Plenum Press, New York, U.S.A.
31. Tempest, D. W. and J. T. M. Wouters. 1981. Properties and performance of microorganisms in chemostat culture. Enz. Microbiol Technol. 3: 283-290.
32. Yamane, T. 1992. Cultivation engineering of microbial bioplastics production. FEMS Microbiol. Rev. 103: 257-264.
33. Yamane, T. 1993. Yield of poly-D(-)-3-hydroxybutyrate from various carbon sources: A theoretical study. Biotech. Bioeng. 41: 165-170.
34. Yoshiharu, W., M. Emiko, and N. Yoji. 1982. Formation of crystalline δ-endotoxin or poly-β-hydroxybutyric acid granules by asporogenous mutants of Bacillus thuringiensis. Appl. Env. Microbiol. 43: 1473-1480.