DNA sequence analysis of hprt mutants persisting in peripheral blood of cynomolgus monkeys more than two years after ENU treatment

Environmental and Molecular Mutagenesis

Volumn 33, Issue 1, 1999, Pages 42-48

  Harbach, P R ^a   Mattano, S S ^a   Zimmer, D M ^a   Filipunas, A L ^a   Wang, Y ^a   Aaron, C S ^a  

^a PFIZER INC   (United States)

Author keywords

DNA sequencing; ENU; Hprt; In vivo mutations; Monkey; T cell clones

Indexed keywords

ETHYLNITROSOUREA; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; TIOGUANINE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASE PAIRING; DNA SEQUENCE; FEMALE; GENE AMPLIFICATION; GENE DELETION; INTRAPERITONEAL DRUG ADMINISTRATION; MOLECULAR CLONING; MONKEY; NONHUMAN; NUCLEOTIDE SEQUENCE; PERIPHERAL CIRCULATION; SEQUENCE ANALYSIS; T LYMPHOCYTE;

ANIMALS; CLONE CELLS; CODON; DNA MUTATIONAL ANALYSIS; ETHYLNITROSOUREA; FEMALE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MACACA FASCICULARIS; MUTAGENESIS, SITE-DIRECTED; MUTAGENS; MUTATION; SEQUENCE DELETION; T-LYMPHOCYTES; TIME FACTORS;

EID: 0032953530     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1999)33:1<42::AID-EM5>3.0.CO;2-P     Document Type: Article

References (40)

1. Aaron CS, Zimmer DM, Harbach PR, Yu RL. 1996. End points for biomonitoring: Assay sensitivity/selectivity. Environ Health Perspect 104(3):521-525.
2. Aidoo A, Lyn-Cook LE, Mittelstaedt RA, Heflich RH, Casciano DA. 1991. Induction of 6-thioguanine-resistant lymphocytes in Fischer 344 rats following in vivo exposure to N-ethyl-N-nitrosourea and cyclophosphamide. Environ Mol Mutagen 17:141-151.
3. Aidoo A, Lyn-Cook LE, Heflich RH, George EO, Casciano DA. 1993. The effect of time after treatment, treatment schedule and animal age on the frequency of 6-thioguanine-resistant T-lymphocytes induced in Fischer 344 rats by N-ethyl-N-nitrosourea. Mutat Res 298:169-178.
4. Albertini RJ, Castle KL, Borcherding WR. 1982. T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood. Proc Natl Acad Sci USA 79:6617-6621.
5. Albertini RJ, O'Neill JP, Nicklas JA, Heintz NH, Kelleher PC. 1985. Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T-lymphocytes. Nature 316:369-371.
6. Bronstein SM, Cochrane JE, Craft TR, Swenberg JA, Skopek TR. 1991. Toxicity, mutagenicity and mutational spectra of N-ethyl-N-nitrosourea in human cell lines with different DNA repair phenotypes. Cancer Res 51:5188-5197.
7. 6-alkylguanine-DNA alkyltransferase and nucleotide excision repair activities in human cells. Cancer Res 52:2008-2011.
8. Cariello NF. 1994. Software for the analysis of mutations at the human hprt gene. Mutat Res 312:173-185.
9. Cariello NF, Skopek TR. 1993. Analysis of mutations occurring at the human hprt locus. J Mol Biol 231:41-57.
10. 4-ethylthymine and the ethylphosphotriester dTp(Et)dT are highly persistent DNA modifications in slowly dividing tissues of the ethylnitrosourea-treated rat. Carcinogenesis 8:751-757.
11. Fuscoe JC, Zimmerman LJ, Lippert MJ, Nicklas JA, O'Neill JP, Albertini RJ. 1991. V(D)J recombinase-like activity mediates hprt gene deletion in human fetal T-lymphocytes. Cancer Res 51:6001-6005.
12. Fuscoe JC, Zimmerman LJ, Harrington-Brock K, Burnette L, Moore MM, Nicklas JA, O'Neill JP, Albertini RJ. 1992. V(D)J recombinase-mediated deletion of the hprt gene in T-lymphocytes from adult humans. Mutat Res 283:13-20.
13. Green MHL, O'Neill JP, Cole J. 1995. Suggestions concerning the relationship between mutant frequency and mutation rate at the hprt locus in human peripheral T-lymphocytes. Mutat Res 334:323-339.
14. Harbach PR, Filipunas AL, Wang Y, Aaron CS. 1992. DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes. Environ Mol Mutagen 20:96-105.
15. Harbach PR, Mattano SS, Zimmer DM, Wang Y, Aaron CS. 1995a. DNA sequence analysis of spontaneous hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes. Environ Mol Mutagen 26: 218-225.
16. Harbach PR, Zimmer DM, Mattano SS, Wang Y, Aaron CS. 1995b. DNA sequence analysis of hprt mutants persisting in peripheral blood of cynomolgus monkeys more than two years after ENU treatment. Environ Mol Mutagen 25(Suppl 25):22.
17. Horsfall MJ, Gordon AJE, Burns PA, Zielenska M, van der Vliet GME, Glickman BW. 1990. Mutational specificity of alkylating agents and the influence of DNA repair. Environ Mol Mutagen 15:107-122.
18. Jansen JG, van Teijllingen CMM, Mohn GR, van Zeeland AA, Vrieling H, 1994a. AT base pairs are the main target for mutations at the hprt locus of rat skin fibroblasts exposed in vitro to the monofunctional alkylating agent N-ethyl-N-nitrosourea. Mutagenesis 9:417-421.
19. Jansen JG, Mohn GR, Vrieling H, van Teijllingen CMM, Lohman PHM, van Zeeland AA. 1994b. Molecular analysis of hprt gene mutations in skin fibroblasts of rats exposed in vivo to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea. Cancer Res 54:2478-2485.
20. Jansen JG, de Groot AJL, van Teijllingen CMM, Lohman PHM, Mohn GR, Vrieling H, van Zeeland AA. 1994c. Formation and persistence of DNA adducts in pouch skin fibroblasts and liver tissue of rats exposed in vivo to the monofunctional alkylating agents N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea. Mutat Res 307:95-105.
21. 6-alkylguanine in hprt mutagenesis in T-lymphocytes of rats exposed in vivo to ethylmethanesulfonate, N-(2-hydroxyethyl)-N-nitrosourea, or N-ethyl-N-nitrosourea. Cancer Res 55:1875-1882.
22. Jones IM, Burkhart-Schultz K, Crippen TL. 1987. Cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo. Somat Cell Mol Genet 13:325-333.
23. Mattano SS, Zimmer DM, Harbach PR, Hunter TC, Aaron CS. 1995. Southern blot analysis of T-cell receptor gene rearrangements in cynomolgus monkeys, and identification of a progenitor cell HPRT mutation. Environ Mol Mutagen 26:119-126.
24. McGregor WG, Maher VM, McCormick JJ. 1991. Kinds and locations of mutations arising spontaneously in the coding region of the HPRT gene of finite-life-span diploid human fibroblasts. Somat Cell Mol Genet 17:463-469.
25. Mittelstaedt RA, Smith BA, Heflich RH. 1995. Analysis of in vivo mutation induced by N-ethyl-N-nitrosourea in the hprt gene of rat lymphocytes. Environ Mol Mutagen 26:261-269.
26. Morley AA, Trainor KJ, Seshadri R, Ryall RG. 1983. Measurement of in vivo mutations in human lymphocytes. Nature 302:155-156.
27. 4-alkylthymidine following exposure to methylating and ethylating hepatocarcinogens. Carcinogenesis 6:625-629.
28. Richardson KK, Richardson FC, Crosby RM, Swenberg JA, Skopek TR. 1987. DNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea. Proc Natl Acad Sci USA 84:344-348.
29. Shibuya T, Morimoto K. 1993. A review of the genotoxicity of 1-ethyl-1-nitrosourea. Mutat Res 297:3-38.
30. 1 mice exposed to N-ethyl-N-nitrosourea. Proc Natl Acad Sci USA 89:7866-7870.
31. Steingrimsdottir H, Rowley G, Dorado G, Cole J, Lehmann AR. 1992. Mutations which alter splicing in the human hypoxanthine guanine phosphoribosyl transferase gene. Nucleic Acids Res 20:1201-1208.
32. Stout JT, Caskey CT. 1985. HPRT: Gene structure, expression, and mutation. Annu Rev Genet 19:127-148.
33. 6-ethyldeoxyguanosine, accumulates in hepatocyte DNA of rats exposed continuously to diethylnitrosamine. Proc Natl Acad Sci USA 81: 1692-1695.
34. Tates AD, van Dam FJ, Natarajan AT, Zwinderman AH, Osanto S. 1994. Frequencies of HPRT mutants and micronuclei in lymphocytes of cancer patients under chemotherapy: A prospective study. Mutat Res 307:293-306.
35. Thayer RM, Power MD, Bryant ML, Gardner MB, Barr PJ, Luciw PA. 1987. Sequence relationships of type D retrovirus which cause simian acquired immunodeficiency syndrome. Virology 157:317-329.
36. - diploid human fibroblasts. Carcinogenesis 15:939-945.
37. Zhang L-H, Vrieling H, van Zeeland AA, Jenssen D. 1992. Spectrum of spontaneously occurring mutations in the hprt gene of V79 Chinese hamster cells. J Mol Biol 223:627-635.
38. Zimmer DM, Aaron CS. 1997. In vivo mutagenesis in the cynomolgus monkey: Time course of HPRT mutant frequency at long time points following ENU exposure. Environ Mol Mutagen 29:117-123.
39. Zimmer DM, Aaron CS, O'Neill JP, Albertini RJ. 1991. Enumeration of 6-thioguanine-resistant T-lymphocytes in the peripheral blood of nonhuman primates (cynomolgus monkeys). Environ Mol Mutagen 18:161-167.
40. Zimmer DM, Mattano SS, Wang Y, Aaron CS. 1995. Persistence of ENU-induced hprt mutants in cynomolgus monkeys and the effect of split dose treatment on mutant frequency. Environ Mol Mutagen 25(Suppl 25):60.