Highly sensitive radioactive in situ hybridization using full length hydrolyzed riboprobes to detect α2 adrenoceptor subtype mRNAs in adult and developing rat brain

Brain Research Protocols

Volumn 3, Issue 3, 1999, Pages 229-241

  Winzer Serhan, Ursula H ^a   Broide, Ron S ^a   Chen, Yiling ^a   Leslie, Frances M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Adrenergic receptor; Development; Ontogeny; Riboprobe

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; MESSENGER RNA; PROTEINASE K; RECEPTOR PROTEIN; RECEPTOR SUBTYPE; SULFUR 35;

ADOLESCENT; ANIMAL TISSUE; ARTICLE; BINDING SITE; BRAIN DEVELOPMENT; CLINICAL PROTOCOL; EMBRYO; HYDROLYSIS; IN SITU HYBRIDIZATION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; RAT; RNA PROBE;

ANIMALS; AUTORADIOGRAPHY; BRAIN; BRAIN CHEMISTRY; FROZEN SECTIONS; IN SITU HYBRIDIZATION; NERVE TISSUE PROTEINS; OLIGONUCLEOTIDE PROBES; PROTEIN ISOFORMS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, ADRENERGIC, ALPHA-2; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; SULFUR RADIOISOTOPES; TISSUE FIXATION;

EID: 0032951233     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(98)00043-9     Document Type: Article

References (17)

1. Broide R.S., O'Conner L.T., Smith M.A., Smith J.A.M., Leslie F.M. Developmental expression of α7 neuronal nicotinic receptor messenger RNA in rat sensory cortex and thalamus. Neuroscience. 67:1995;83-94.
2. Cox K.H., DeLeon D.V., Angerer L.M., Angerer R.C. Detection of mRNA in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101:1984;485-502.
3. 2-adrenoceptors. TIPS. 12:1991;62-67.
4. Jensen J.J., Winzer-Serhan U.H., Leslie F.M. Glial regulation of α7-type nicotinic acetylcholine receptor expression in cultured rat cortical neurons. J. Neurochem. 68:1997;112-120.
5. Le Novere N., Zoli M., Changeux J.-P. Neuronal nicotinic receptor α6 subunit mRNA is selectively concentrated in catecholaminergic nuclei of the rat brain. Eur. J. Neurosci. 8:1996;2428-2439.
6. McCabe J.T., Pfaff D.W. In situ hybridization: a methodological guide. Methods Neurosci. 1:1989;98-126.
7. Meister B., Dagerlind A., Nicholas A.P., Hökfelt T. Patterns of messenger RNA expression for adrenergic receptor subtypes in rat kidney. J. Pharm. Exp. Ther. 268:1994;1605-1611.
8. 14C plastic standards for quantitative autoradiography using LKB ultrofilm or Amersham hyperfilm Neurosci. Lett. 121:1991;211-214.
9. Nicholas A.P., Hökfelt T., Pieribone V. The distribution and significance of CNS adrenoceptors examined with in situ hybridization. TIPS. 17:1996;245-255.
10. R.D. O'Shea, A.L. Gundlach, Quantitative analysis of in situ hybridization histochemistry, in: W. Wisden, B.J. Morris (Eds.), Biological techniques, In situ hybridization protocols for the brain, Acad. Press, 1994, pp. 57-78.
11. Simmons D.M., Arriza J.L., Swanson L.W. A complete protocol for in situ hybridization of messenger RNA in brain and other tissues with radiolabeled single-strand RNA probes. J. Histotechnol. 12:1989;169-181.
12. Wilcox J.N. Fundamental principles of in situ hybridization. J. Histochem. Cytochem. 41:1993;1725-1733.
13. 2B adrenoceptor mRNA expression during rat brain development. Dev. Brain Res. 100:1997;90-100.
14. Winzer-Serhan U.H., Leslie F.M. Codistribution of nicotinic acetylcholine receptor subunit alpha3 and beta4 mRNAs during rat brain development. J. Comp. Neurol. 386:1997;540-554.
15. 2A messenger RNA expression. Neuroscience. 76:1997;241-259.
16. 3H ]Rauwolscine binding Neuroscience. 76:1997;261-272.
17. W. Wisden, B.J. Morris, In situ hybridization with synthetic oligonucleotide probes, in: W. Wisden, B.J. Morris (Eds.), Biological techniques, In situ hybridization protocols for the brain, Acad. Press, 1994, pp. 9-34.