Cell electroporation: Estimation of the number of pores and their sizes

Biomedical Sciences Instrumentation

Volumn 35, Issue , 1999, Pages 291-296

  Saulis, Gintautas ^a  

^a VYTAUTAS MAGNUS UNIVERSITY   (Lithuania)

Author keywords

Electropermeabilization; Electroporation; Erythrocytes; Pore number; Pore size

Indexed keywords

CELLS; ELECTRIC FIELD EFFECTS; ELECTROPHYSIOLOGY; POROUS MATERIALS;

CELL ELECTROPORATION; ELECTROPERMEABILIZATION;

CELL MEMBRANES;

ASCORBIC ACID; MANNITOL; SUCROSE;

CELL KINETICS; COLLOID; CONFERENCE PAPER; CYTOLYSIS; ELECTRIC FIELD; ELECTROPORATION; ERYTHROCYTE; HUMAN; HUMAN CELL; ONCOTIC PRESSURE; OSMOTIC PRESSURE; POROSITY;

ASCORBIC ACID; CELL MEMBRANE PERMEABILITY; ELECTROPORATION; ERYTHROCYTE MEMBRANE; HUMANS; POROSITY;

EID: 0032951013     PISSN: 00678856     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (26)

1. Sale, A.J.H., and W.A. Hamilton. 1967. Effects of high electric fields on microorganisms. I. Killing of bacteria and yeasts. Biochim. Biophys. Acta. 148:781-788.
2. Neumann, E., and K. Rosenheck. 1972. Permeability changes induced by electric impulses in vesicular membranes. J. Membrane Biol. 10:279-290.
3. Kinosita, K., and T. Y. Tsong. 1977. Formation and resealing of pores of controlled sizes in human erythrocyte membrane. Nature. 268:438-441.
4. Schwister, K., and B. Deuticke. 1985. Formation and properties of aqueous leaks induced in human erythrocytes by electrical breakdown. Biochim. Biophys. Acta. 816:332-348.
5. Weaver, J C. 1993. Electroporation: a general phenomenon for manipulating cells and tissues. J. Cell Biochem. 51:426-435.
6. Zimmermann, U. 1982. Electric field mediated fusion and related electrical phenomena. Biochim. Biophys. Acta. 694:227-277.
7. Tsong, T.Y. 1990. On electroporation of cell membranes and some related phenomena. Bioelectrochem. Bioenerg. 24:271-295.
8. Sale, A.J.H., and W.A. Hamilton. 1968. Effects of high electric fields on microorganisms. III. Lysis of erythrocytes and protoplasts. Biochim. Biophys. Acta. 163:37-43.
9. Jarayam, S., G.S.P. Castle, and A. Margaritis. 1992. Kinetics of sterilization of lactobacillus-brevis cells by the application of high voltage pulses. Biotechnol Bioeng. 40:1412-1420.
10. Kinosita, K., and T.Y. Tsong. 1978. Survival of sucrose-loaded erythrocytes in the circulation. Nature. 272:258-260.
11. Knight, D.E., and M.C. Scrutton. 1986. Gaining access to the cytosol: the technique and some applications of electropermeabilization. Biochem. J. 234:497-506.
12. Raptis, L., S.K.-W. Liu, K.L. Firth, C.D. Stiles, and J.A. Alberta. 1995. Electroporation of peptides into adherent cells in situ. Biotechniques. 104-114.
13. Cortes, F., I. Dominguez, and B. Sandström. 1994. Exogenous catalase introduced in CHO cells by electroporation does not protect against chromosome damage induced by ionizing radiation. Cell Biol. Internat. 18:959-965.
14. Senda, M., I. Takeda, S. Abe, and T Nakamura. 1979. Induction of cell fusion of plant protoplasts by electrical stimulation. Plant Cell Physiol. 20:1491-1493.
15. Sowers, A.E. 1995. Permeability alteration by transmembrane electric fields: electroporation. In Permeability and Stability of Lipid Bilayers. E. A. Disalvo and S.A. Simon editors. CRC Press, Boca Raton, pp. 105-121.
16. Neumann, E., M. Schaefer-Ridder, Y. Wang and P.H. Hofschneider 1982 Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1:841-845.
17. Potter, H. 1988. Electroporation in biology: Methods, applications and instrumentation. Anal. Biochem 174:361-373.
18. Ishiguro, J., and W. Kobayashi, 1995. A practical method fo fission yeast transformation by electroporation. Jpn. J. Genet. 70:1-6.
19. Mouneimne Y., P.-F. Tosi, Y. Gazitt, and C. Nicolau. 1989. Electro-insertion of xeno-glycophorin into the red blood cell membrane. Biochem. Biophys Res Commun. 159:34-40.
20. Mir, L.M., S. Orlowski, J. Belehradek, and C. Paoletti. 1991. Electrochemotherapy: potentiation of antitumor effect of bleomycin by local electric pulses. Eur. J. Cancer 27:68-72.
21. Naktinis, J.J. and L.Ch. Cherniauskiene. 1974. On the nature of free radical ESR signal in dry animal tissues. Biofizika. 19:1039-1044. (In Russian.)
22. Kotyk, A., and K. Janacek. 1977. Membrane Transport. Plenum Press, New York.
23. Saulis, G., M.S. Venslauskas, and J. Naktinis. 1991. Kinetics of pore resealing in cell membranes after electroporation. Bioelectrochem. Bioenerg. 26:1-13.
24. Saulis, G., and M.S. Venslauskas. 1993. Cell electroporation. Part 2. Experimental measurements of the kinetics of pore formation in human erythrocytes. Bioelectrochem. Bioenerg. 36:237-248.
25. Kinosita, K., and T. Y. Tsong. 1977. Formation and resealing of pores of controlled sizes in human erythrocyte membrane. Nature 268:438-441.
26. Renkin, E.M. 1954. Filtration, diffusion and molecular sieving through porous cellulose membranes. J Gen. Physiol. 38:225-243.