Hybridocytochemical detection of mRNA for calcitonin, CGRP, somatostatin and NPY in cultured cells of medullary thyroid carcinoma using immunomax technique

Folia Histochemica et Cytobiologica

Volumn 37, Issue 2, 1999, Pages 59-60

  Kasprzak, A ^a   Zabel, M ^a,b   Surdyk Zasada, J ^a   Seidel, J ^a  

^a POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^b MEDICAL UNIVERSITY OF LUBLIN   (Poland)

Author keywords

Biotinylated tyramine; Calcitonin; CGRP; Hybridocytochemistry; mRNA; NPY; Somatostatin; TT cells

Indexed keywords

CALCITONIN; CALCITONIN GENE RELATED PEPTIDE RECEPTOR; ENKEPHALIN; MESSENGER RNA; NEUROPEPTIDE Y; NEUROTENSIN; PARATHYROID HORMONE RELATED PROTEIN; PEPTIDE HORMONE; SOMATOSTATIN;

ARTICLE; CANCER CELL CULTURE; HORMONE RELEASE; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; PROTEIN EXPRESSION; RNA ANALYSIS; THYROID MEDULLARY CARCINOMA; THYROID PARAFOLLICULAR CELL;

EID: 0032950962     PISSN: 00155586     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Kasprzak A, Seidel J, Zabel M (1996) Demonstration of calcitonin mRNA in rat thyroid C-cells and endocrine cells of the lung: comparison of immunocytochemical and hybridocytochemical results. Folia Histochem Cytobiol 34, Suppl 1: 9-10
2. Koji T, Kanemitsu Y, Hoshino A, Nakane PK (1997) A novel amplification method of nonradioactive in situ hybridization signal for specific RNA with biotinylated tyramine. Acta Histochem Cytochem 30: 401-406
3. Seidel J, Zabel M, Kaczmarek A, Grzeszkowiak J, Kasprzak A (1996) Immunocytochemical localization of hormones and functional proteins in cultured cells of thyroid medullary carcinoma. Folia Histochem Cytobiol 34, Suppl 1: 67-68
4. Wilcox JN (1993) Fundamental principles of in situ hybridization. J Histochem Cytochem 41: 1055-1060
5. Zabel M (1984) Ultrastructural localization of calcitonin, somatostatin and scrotonin in parafollicular cells of rat thyroid. Histochem J 16: 1265-1272
6. Zabel M, Seidel J, Kaczmarek A, Surdyk-Zasada J, Grzeszkowiak J, Górny A (1994) Hybridocytochemical and immuno-ultrastructural study of calcitonin gene expression in cultured medullary carcinoma cells. Histochemistry 102: 323-327
7. Zabel M, Seidel J, Kaczmarek A, Surdyk-Zasada J, Grzeszkowiak J, Górny A (1995) Immunocytochemical characterization of two thyroid medullary carcinoma cell lines in vitro. Histochem J 27: 859-868