Preparation of isomerized α-acid standards for HPLC analysis of iso-α-acids, rho-iso-α-acids, tetrahydro-iso-α-acids, and hexahydro-iso-α-acids

Journal of the American Society of Brewing Chemists

Volumn 57, Issue 2, 1999, Pages 55-59

  Maye, John Paul ^a   Mulqueen, Susie ^a   Weis, Scot ^a   Xu, Jianping ^a   Priest, Matthew ^a,b  

^a Haas Hop Products Inc   (United States)

^b PFIZER INC   (United States)

Author keywords

Dicyclohexylamine

Indexed keywords

ACID; BREWING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PURIFICATION; SALT;

EID: 0032948975     PISSN: 03610470     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. American Society of Brewing Chemists. Report of subcommittee on determination of isohurnulones in beer. Proc. 25:269, 1967.
2. American Society of Brewing Chemists. Report of subcommittee on determination of isohurnulones in beer. Proc. 26:280, 1968.
3. American Society of Brewing Chemists. Methods of Analysis, 8th ed. Beer-23. The Society, St. Paul, MN, 1992.
4. Biendl, M., and Hartl, A. Dicyclohexylomine-trans-iso-α-acid complex as esternal calibration standard for HPLC determination of iso-α-acids. Monatsschr. Brauwiss. 3/4:102, 1995.
5. Brown, P. M., Howard, G. A., and Tatchell, A. R. Chemistry of hop constituents J. Chem. Soc. 112: 545, 1959.
6. Buckee, G. K. Determination of iso-alpha-, alpha-, and beta-acids in hop and isomerized hop extracts: A collaborative trial. J. Inst. Brew. 100:151, 1994.
7. Donnally, W. J. G., and Shannon, P. V. R. cis-and trans-tetrahydroisohumulones. J. Chem. Soc. (C) 1970:524, 1970.
8. European Brewery Convention. Analytica EBC. Section 7 Hops. Method 7.8, 1998.
9. Hay, B. A., Homiski, J. W., and Howie, J. L. Differences in ultraviolet absorbance of tetrahydroiso-α-acid components J. Am. Soc. Brew. Chem. 52:54, 1994.
10. Hay, B. A., Homiski, J. W., and Priest, M. A. A simplified method for synthesis of tetrahydroiso-α-acid standards for high-performance liquid chromatography J. Am. Soc. Brew. Chem. 49:115, 1991.
11. Howard, G. A. Estimation of the bitterness of beer J. Inst. Brew. 74:249, 1968.
12. Rigby, F. L., and Bethune, J. L. Rapid methods for the determination of total hop bitter substances (iso-compounds) in beer. J. Inst. Brew. 61:325-332, 1955.
13. Thornton, H. A., Kulandai, J., Bond, M., Jontef, M. P., Hawthorne, D. B., and Kavanagh, T. E. Preparation of a trans-iso-α-acids and use of their dicyclohexylamine salts as a standard for iso-α-acids analysis. J. Inst. Brew. 96:473-477, 1993.
14. Thornton, H. A., Kulandal, J., Hawthorne, D. B., and Kavanagh, T. E. Preparation of a trans-isohumulone standard. J. Inst. Brew. 96:367, 1990.
15. Todd, P. H., Johnson, P. A., and Worden, L. R. Evaluation of the relative bitternes and light stability of reduced iso-α-acids. MBAA Tech. Q. 9:31, 1972.