The effect of saliva specimen collection, handling and storage protocols on hepatitis C virus (HCV) RNA detection by PCR

Oral Diseases

Volumn 5, Issue 2, 1999, Pages 123-127

  Roy, K M ^a   Bagg, J ^a   McCarron, B ^b  

^a GLASGOW DENTAL HOSPITAL AND SCHOOL   (United Kingdom)

^b GARTNAVEL GENERAL HOSPITAL   (United Kingdom)

Author keywords

Hepatitis C virus; Oral fluid; Polymerase chain reaction; Whole saliva

Indexed keywords

VIRUS RNA;

ADULT; ARTICLE; CLINICAL ARTICLE; ENZYME IMMUNOASSAY; FEMALE; HEPATITIS C VIRUS; HUMAN; IMMUNOBLOTTING; LABORATORY DIAGNOSIS; MALE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALIVA ANALYSIS; VIRUS DETECTION;

EID: 0032948458     PISSN: 1354523X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1601-0825.1999.tb00076.x     Document Type: Article

References (21)

1. Busch MP, Wilber JC, Johnson P et al (1992). Impact of specimen handling and storage on detection of hepatitis C virus RNA. Transfusion 32: 420-425.
2. Cameron SO (1997). Diagnostic perspective of viral hepatitis. Rev Med Microbiol 8: 197-207.
3. Chen M, Yun ZB, Sallberg M et al (1995). Detection of hepatitis C virus RNA in the cell fraction of saliva before and after oral-surgery. J Med Virol 45: 223-226.
4. Cuypers HTM, Bresters D, Winkel IN et al (1992). Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C virus. J Clin Microbiol 30: 3220-3224.
5. Fried MW, Shindo M, Fong TL et al (1992). Absence of hepatitis C viral RNA from saliva and semen of patients with chronic hepatitis C. Gastroenterol 102: 1306-1308.
6. Halfon P, Khiri H, Gerolami V et al (1996). Impact of various handling and storage conditions on quantitative detection of hepatitis C virus RNA. J Hepatol 25: 307-311.
7. Hsu HH, Wright TL, Luba D et al (1991). Failure to detect hepatitis C virus genome in human secretions with the polymerase chain reaction. Hepatol 14: 763-767.
8. Liou TC, Chang TT, Young KC et al (1992). Detection of HCV RNA in saliva, urine, seminal fluid, and ascites. J Med Virol 37: 197-202.
9. Malamud D, Tabak L (1993). Saliva as a diagnostic fluid. Ann N Y Acad Sci 694: 1-347.
10. McIntyre PG, Laszlo J, Appleyard K et al (1996). Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid. Eur J Clin Microbiol Infect Dis 15: 882-884.
11. Mortimer PP, Parry JV (1988). The use of saliva for viral diagnosis and screening. Epidemiol Infect 101: 197-200.
12. Ochert AS, Boulter AW, Birnbaum W et al (1994). Inhibitory effect of salivary fluids on PCR: potency and removal. PCR Methods and Applications 3: 365-368.
13. Roy KM, Bagg J, Follett EA et al (1996). Hepatitis C virus in saliva of haemophiliac patients attending an oral surgery unit. Br J Oral Maxillofac Surg 34: 162-165.
14. Roy KM, Bagg J, McCarron B et al (1998). Predominance of HCV type 2a in saliva from intravenous drug users. J Med Virol 54: 271-275.
15. Saldanha J, Minor P (1996). Collaborative study to assess the suitability of an HCV RNA reference sample for detection of HCV RNA in plasma pools by PCR. Vox Sang 70: 148-151.
16. Wang JT, Wang TH, Lin JT et al (1991). Hepatitis C virus RNA in saliva of patients with post-transfusion hepatitis C infection (Letter). Lancet 337: 48.
17. Wang JT, Wang TH, Sheu JC et al (1992a). Hepatitis C virus RNA in saliva of patients with post-transfusion hepatitis and low efficiency of transmission among spouses. J Med Virol 36: 28-31.
18. Wang JT, Wang TH, Sheu JC et al (1992b). Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus. J Clin Microbiol 30: 750-753.
19. Young KC, Chang TT, Liou TC et al (1993). Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and saliva. J Med Virol 41: 55-60.
20. Zaaijer HL, Cuypers HTM, Reesink HW et al (1993). Reliability of polymerase chain reaction for detection of hepatitis C virus. Lancet 341: 722-724.
21. Zaaijer HL, Damen M, Cuypers HTM et al (1995). 2nd international quality-control study on detection of HCV RNA (Eurohep-2). Transfusion 35: S132.