γ-tubulin in Chlamydomonas: Characterization of the gene and localization of the gene product in cells

Cell Motility and the Cytoskeleton

Volumn 42, Issue 4, 1999, Pages 285-297

  Silflow, C D ^a   Liu, B ^b,c   LaVoie, M ^a   Richardson, E A ^b   Palevitz, B A ^b  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

tubulin; Basal body; Chlamydomonas; Microtubule organizing center

Indexed keywords

TUBULIN;

ANIMAL CELL; ARTICLE; CELL MOTILITY; CENTRIOLE; CHLAMYDOMONAS; CYTOSKELETON; GENE ISOLATION; GENE SEQUENCE; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

ALGAE; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CENTROSOME; CHLAMYDOMONAS REINHARDTII; CHROMOSOME MAPPING; CLONING, MOLECULAR; COS CELLS; DNA PRIMERS; DNA, COMPLEMENTARY; GENES, PLANT; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; TUBULIN;

ALGAE; ANIMALIA; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; EMBRYOPHYTA;

EID: 0032948132     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1999)42:4<285::AID-CM3>3.0.CO;2-Z     Document Type: Article

References (67)

1. Cavalier-Smith T. 1974. Basal body and flagellar development during the vegetative cell cycle and the sexual cycle of Chlamydomonas reinhardtii. J Cell Sci 16:529-556.
2. Coss RA. 1974. Mitosis in Chlamydomonas reinhardtii basal bodies and the mitotic apparatus. J Cell Biol 63:325-329.
3. Dentier WL. 1990. Linkages between microtubules and membranes in cilia and flagella. In Bloodgood RA, editor. Ciliary and flagellar membranes. New York: Plenum Press, p 31-64.
4. Dibbayawan TP, Harper JDI, Elliot JE, Gunning BES, Marc J. 1995. A γ-tubulin that associates specifically with centrioles in Hela cells and the basal body complex in Chlamydomonas. Cell Biol Int 19:559-567.
5. Doonan JH, Grief C. 1987. The microtubule cycle in Chlamydomonas reinhardtii: an immunofluorescence study. Cell Motil Cytoskeleton 7:381-392.
6. Dutcher SK, Trabuco EC. 1998. The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes δ-tubulin, a new member of the tubulin superfamily. Mol Biol Cell 9:1293-1308.
7. Ehler LL, Holmes JA, Dutcher SK. 1995. Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics 141:945-960.
8. Enkerli K, Hahn MG, Mims CW. 1997. Immunogold localization of callose and other plant cell wall components in soybean roots infected with the oomycete Phytophthora sojae. Can J Bot 75:1509-1517.
9. Felix A-M, Antony C, Wright M, Maro B. 1994. Centrosome assembly in vitro: role of γ-tubulin recruitment in Xenopus sperm aster formation. J Cell Biol 124:19-31.
10. Freshour G, Clay RP, Fuller MS, Albersheim P, Darvill AG, Hahn MG. 1996. Development and tissue-specific structural alternations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol 110:1413-1429.
11. Fuller SD, Gowen BE, Reinsch S, Sawyer Al, Buendia B, Wepf R, Karsenti E. 1995. The core of the mammalian centriole contains γ-tubulin. Curr Biol 5:1384-1393.
12. Gaffal KP. 1988. The basal body-root complex of Chlamydomonas reinhardtii during mitosis. Protoplasma 143:118-129.
13. Gaffal KP, el-Gammal S. 1990. Elucidation of the enigma of the "metaphase band" of Chlamydomonas reinhardtii. Protoplasma 156:139-148.
14. Goodenough UW, Weiss RL. 1978. Interrelationships between microtubules, a striated fiber, and the gametic mating structure of Chlamydomonas reinhardtii. J Cell Biol 76:430-438.
15. Gorman DS, Levine RP. 1965. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 54:1665-1669.
16. Hoch H. 1986. Freeze-substitution in fungi. In: Aldrich HC, Todd WJ, editors. Ultrastructure techniques for microorganisms. New York: Plenum Press. p 183-212.
17. Holmes JA, Dutcher SK. 1989a. Cellular asymmetry in Chlamydomonas reinhardtii. J Cell Sci 94:273-285.
18. Holmes JA, Dutcher SK. 1989b. Genetic approaches to the study of cytoskeletal structure and function in Chlamydomonas. In: Menzel D, editor. The cytoskeleton of algae. Boca Raton, FL: CRC Press. p 347-367.
19. Hoops HJ, Witman GB. 1983. Outer doublet heterogeneity reveals structural polarity related to beat direction in Chlamydomonas flagella. J Cell Biol 97:902-908.
20. Horio T, Uzawa S, Jung MK, Oakley BR, Tanaka K, Yanagida M. 1991. The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers. J Cell Sci 99:693-700.
21. Huang BP-H. 1986. Chlamydomonas reinhardtii: a model system for the genetic analysis of flagellar structure and motility. Int Rev Cytol 99:181-215.
22. Jarvik JW, Suhan JP. 1991. The role of the flagellar transition region: inferences from the analysis of a Chlamydomonas mutant with defective transition region structure. J Cell Sci 99:731-740.
23. Johnson U, Porter KR. 1968. Fine structure of cell division in Chlamydomonas reinhardtii. Basal bodies and microtubules. J Cell Biol 38:403-425.
24. Joshi HC. 1994. Microtubule organizing centers and γ-tubulin. Curr Opin Cell Biol 6:55-62.
25. Joshi HC, Palevitz BA. 1996. γ-Tubulin and microtubule organization in plants. Trends Cell Biol 6:41-44.
26. Julian M, Tollon Y, Lajoie-Mazenc I, Moisand A, Mazarguil H, Puget A, Wright M. 1993. γ-Tubulin participates in the formation of the midbody during cytokinesis in mammalian cells. J Cell Sci 105:145-156.
27. Koerner TJ, Hill JE, Myers AM, Tzagoloff A. 1991. High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors. Methods Enzymol 194:477-490.
28. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4, Nature 227:680-685.
29. Lechtreck K-F, Silflow CD. 1997. SF-assemblin in Chlamydomonas: sequence conservation and localization during the cell cycle. Cell Motil Cytoskeleton 36:190-201.
30. LeDizet M, Piperno G. 1986. Cytoplasmic microtubules containing acetylated α-tubulin in Chlamydomonas reinhardtii: spatial arrangement and properties. J Cell Biol 103:13-22.
31. Liang A, Ruiz F, Heckman K, Klotz C, Tollon Y, Beisson J, Wright M. 1996. Gamma-tubulin is permanently associated with basal bodies in ciliates. Eur J Cell Biol 70:331-338.
32. Liu B, Joshi HC, Wilson TJ, Silflow CD, Palevitz BA, Snustad DP. 1994. γ-Tubulin in Arabidopsis: gene sequence, immunoblot, and immunofluorescence studies. Plant Cell 6:303-314.
33. Liu B, Cyr RJ, Palevitz BA. 1996. A kinesin-like protein, KatAp, in the cells of Arabidopsis and other plants. Plant Cell 8:119-132.
34. Marschall L, Jeng R, Mulholland J, Stearns T. 1996. Analysis of Tub4p, a yeast γ-tubulin-like protein: implication for microtubule organizing center function. J Cell Biol 134:443-454.
35. Martin MA, Osmani SA, Oakley BR. 1997. The role of γ-tubulin in mitolic spindle formatin and cell cycle progression in Aspergillus nidulans. J Cell Sci 110:623-633.
36. Martin OC, Gunawardane RN, Iwamatsu A, Zheng Y. 1998. Xgrip109: a γ-tubulin-associated protein with an essential role in γ-tubulin ring complex (gTuRC) assembly and centrosome function. J Cell Biol 141:675-687.
37. Moritz M, Braunfeld MB, Sedat JW, Alberts B, Agard DA. 1995. Microtubule nucleation by γ-tubulin-containing rings in the centrosome. Nature 378:638-640.
38. Muresan V, Joshi HC, Besharse JC. 1993. γ-Tubulin in differentiated cell types: localization in the vicinity of basal bodies in retinal photoreceptors and ciliated epithelia. J Cell Sci 104:1229-1237.
39. Murphy SM, Urbani L, Stearns T. 1998. The mammalian γ-tubulin complex contains homologues of the yeast spindle pole body components Spc97p and Spc98p. J Cell Biol 141:663-674.
40. Nelson JAE, Savereide PB, Lefebvre BR. 1994. The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. Mol Cell Biol 14:4011-4019.
41. Oakley CE, Oakley BR. 1989. Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature 338:662-664.
42. Palacios MJ, Joshi HC, Simerly C, Schatten G. 1993. Gamma-tubulin reorganization during mouse fertilization and early development. J Cell Sci 104:383-389.
43. Ranum LPW, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD. 1988. Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122.
44. Reynolds ES. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208-212.
45. Ringo DL. 1967. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J Cell Biol 33:543-571.
46. Rowley JC III, Moran DT. 1975. A simple procedure for mounting wrinkle-free sections on formvar-coated slot grids. Ultramicroscopy 1:151-155.
47. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
48. Schnackenberg BJ, Khodjakov A, Rieder CL, Palazzo RE. 1998. The disassembly and reassembly of functional centrosomes in vitro. Proc Natl Acad Sci USA 95:9295-9300.
49. Schnell RA, Lefebvre PA. 1993. Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging. Genetics 134:737-747.
50. Scott V, Sherwin T, Gull K. 1997. γ-Tubulin in trypanosomes: molecular characterization and localisation to multiple and diverse microtubule organizing centers. J Cell Sci 110:157-168.
51. Silflow CD. 1998. Organization of the nuclear genome. In: Rochaix J-D, Goldschmidt-Clermont M, Merchant S, editors. Molecular biology of Chlamydomonas: chloroplasts and mitochondria. Amsterdam: Kluwer Academic Publisher, p 25-40.
52. Silflow CD, Chisholm RL, Conner TW, Ranum LPW. 1985. The two α-tubulin genes of Chlamydomonas reinhardtii code for slightly different proteins. Mol Cell Biol 5:2389-2398.
53. Smith EF, Lefebvre PA. 1997. The role of central apparatus components in flagellar motility and microtubule assembly. Cell Motil Cytoskeleton 38:1-8.
54. Sobel SG, Snyder M. 1995. A highly divergent γ-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae. J Cell Biol 131:1775-1788.
55. Stearns T, Kirschner M. 1994. In vitro reconstitution of centrosome assembly and function: the central role of γ-tubulin. Cell 76:623-637.
56. Stearns MA, Connolly JA, Brown DL. 1976. Cytoplasmic microtubule organizing centers isolated from Polytomella agilis. Science 191:188-191.
57. Sunkel CE, Gomes R, Sampaio P, Perdigao J, Gonzalez C. 1995. γ-Tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts. EMBO J 14:28-36.
58. Tam JP. 1988. Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proc Natl Acad Sci USA 85:5409-5413.
59. Tam L-W, Lefebvre PA. 1993. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics 135:375-384.
60. Tassin A-M, Celati C, Moudjou M, Bornens M. 1998. Characterization of the human homologue of the yeast Spc98p and its association with γ-tubulin. J Cell Biol 141:689-701.
61. Vassilev A, Kimble M, Silflow CD, LaVoie M, Kuriyama R. 1995. Identification of intrinsic dimer and overexpressed monomeric forms of γ-tubulin in Sf9 cells infected with baculovirus containing the Chlamydomonas γ-tubulin sequence. J Cell Biol 108:1083-1092.
62. Vieira J, Messing J. 1987. Production of single-stranded plasmid DNA. Methods Enzymol 153:3-11.
63. Vogel JM, Stearns T, Rieder CL, Palazzo RE. 1997. Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of α/β tubulin. J Cell Biol 137:193-202.
64. Weiss RL. 1984. Ultrastructure of the flagellar roots in Chlamydomonas gametes. J Cell Sci 67:133-143.
65. Wright RL, Salisbury J, Jarvik JW. 1985. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation. J Cell Biol 101:1903-1912.
66. Zheng Y, Jung K, Oakley BR. 1991. γ-Tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65:817-823.
67. Zheng Y, Wong ML, Alberts B, Mitchison T. 1995. Nucleation of microtubule assembly by a γ-tubulin containing ring complex. Nature 378:578-583.