Requirement of NifX and other nif proteins for in vitro biosynthesis of the iron-molybdenum cofactor of nitrogenase

Journal of Bacteriology

Volumn 181, Issue 9, 1999, Pages 2797-2801

  Shah, Vinod K ^a   Rangaraj, Priya ^a   Chatterjee, Ranjini ^a   Allen, Ronda M ^a   Roll, Jon T ^a   Roberts, Gary P ^a   Ludden, Paul W ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Department of Biochemistry ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARBOXYLIC ACID DERIVATIVE; CITRIC ACID; DINITROGENASE REDUCTASE; DITHIONITE; MOLYBDENUM IRON PROTEIN; MOLYBDIC ACID; NITROGENASE; PROTEIN; PROTEIN NIFB; PROTEIN NIFNE; PROTEIN NIFX; UNCLASSIFIED DRUG;

ARTICLE; AZOTOBACTER VINELANDII; CHEMICAL COMPOSITION; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN DETERMINATION; PROTEIN SYNTHESIS; REACTION ANALYSIS; TECHNIQUE;

AZOTOBACTER VINELANDII; NEGIBACTERIA; TRIXIS;

EID: 0032947995     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.9.2797-2801.1999     Document Type: Article

References (33)

1. Allen, R. M., R. Chatterjee, P. W. Ludden, and V. K. Shah. 1995. Incorporation of iron and sulfur from NifB cofactor into the iron-molybdenum cofactor of dinitrogenase. J. Biol. Chem. 270:26890-26896.
2. 2 structural gene. J. Bacteriol. 171:360-368.
3. Brigle, K. E., R. A. Setterquist, D. R. Dean, J. S. Cantwell, M. C. Weiss, and W. E. Newton. 1987. Site-directed mutagenesis of the nitrogenase MoFe protein of Azotobacter vinelandii. Proc. Natl. Acad. Sci. USA 84:7066-7069.
4. Brigle, K. E., M. C. Weiss, W. E. Newton, and D. R. Dean. 1987. Products of the iron-molybdenum cofactor-specific biosynthetic genes, nifE and nifN, are structurally homologous to the products of the nitrogenase molybdenum-iron protein genes, nifD and nifK. J. Bacteriol. 169:1547-1553.
5. 2 evolution and ATP hydrolysis. Proc. Natl. Acad. Sci. USA 56:979-986.
6. Dean, D. R., J. T. Bolin, and L. Zheng. 1993. Nitrogenase metalloclusters: structures, organization, and synthesis. J. Bacteriol. 175:6737-6744.
7. Filler, W. A., R. M. Kemp, J. C. Ng, T. R. Hawkes, R. A. Dixon, and B. E. Smith. 1986. The nifH gene product is required for the synthesis or stability of the iron-molybdenum cofactor of nitrogenase from Klebsiella pneumoniae. Eur. J. Biochem. 160:371-377.
8. Gosink, M. M., N. M. Franklin, and G. P. Roberts. 1990. The product of the Klebsiella pneumoniae nifX gene is a negative regulator of the nitrogen fixation (nif) regulon. J. Bacteriol. 171:1441-1447.
9. Hageman, R. V., and R. H. Burris. 1978. Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle. Proc. Natl. Acad. Sci. USA 75:2699-2702.
10. Hawkes, T. R., P. A. McLean, and B. E. Smith. 1984. Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor. Biochem. J. 217:317-321.
11. Hill, H. D., and J. G. Straka. 1988. Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents. Anal. Biochem. 170:203-208.
12. Hoover, T. R., J. Imperial, P. W. Ludden, and V. K. Shah. 1989. Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase. Biochemistry 28:2768-2771.
13. Imperial, J., T. R. Hoover, M. S. Madden, P. W. Ludden, and V. K. Shah. 1989. Substrate reduction properties of dinitrogenase activated in vitro are dependent upon the presence of homocittate or its analogues during iron-molybdenum cofactor synthesis. Biochemistry 28:7796-7799.
14. - mutants. J. Bacteriol. 169:1784-1786.
15. Imperial, J., R. A. Ugalde, V. K. Shah, and W. J. Brill. 1984. Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae. J. Bacteriol. 158:187-194.
16. Jacobson, M. R., K. E. Brigle, L. T. Bennett, R. A. Setterquist, M. S. Wilson, V. L. Cash, J. Beynon, W. E. Newton, and D. R. Dean. 1989. Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii. J. Bacteriol. 171:1017-1027.
17. Kim, J., and D. C. Rees. 1992. Crystallographic structure and functional implications of the nitrogenase molybdenum-iron protein from Azotobacter vinelandii. Nature 360:553-560.
18. Ljones, T., and R. H. Burris. 1978. Nitrogenase: the reaction between the Fe protein and bathophenanthrolinedisulfonate as a probe for interactions with MgATP. Biochemistry 17:1866-1872.
19. MacNeil, T., D. MacNeil, G. P. Roberts, M. A. Supiano, and W. J. Brill. 1978. Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae. J. Bacteriol. 136:253-266.
20. Moreno-Vivian, C., M. Schmehl, B. Masepohl, W. Arnold, and W. Klipp. 1989. DNA sequence and genetic analysis of the Rhodobacter capsulatus nifENX gene region: homology between NifX and NifB suggests involvement of NifX in processing of the iron-molybdenum cofactor. Mol. Gen. Genet. 216:353-363.
21. Muchmore, S. W., R. F. Jack, and D. R. Dean. 1996. Developments in the analysis of nitrogenase FeMo-cofactor biosynthesis, p. 111-132. In R. P. Hausinger (ed.), Mechanisms of metallocenter assembly. VCH Publishers, Inc., New York, N.Y.
22. Paustian, T. D., V. K. Shah, and G. P. Roberts. 1990. Apo-dinitrogenase: purification, association with a 20-kilodalton protein, and activation by the iron-molybdenum cofactor in the absence of dinitrogenase reductase. Biochemistry 29:3515-3522.
23. Paustian, T. D., V. K. Shah, and G. P. Roberts. 1989. Purification and characterization of the nifN and nifE gene products from Azotobacter vinelandii mutant UW45. Proc. Natl. Acad. Sci. USA 86:6082-6086.
24. Rawlings, J., V. K. Shah, J. R. Chisnell, W. J. Brill, R. Zimmermann, E. Münck, and W. H. Orme-Johnson. 1978. Novel metal cluster in the iron-molybdenum cofactor of nitrogenase. J. Biol. Chem. 253:1001-1004.
25. Robinson, A. C., D. R. Dean, and B. K. Burgess. 1987. Iron-molybdenum cofactor biosynthesis in Azotobacter vinelandii requires the iron protein of nitrogenase. J. Biol. Chem. 262:14327-14332.
26. Roll, J. T., V. K. Shah, D. R. Dean, and G. P. Roberts. 1995. Characteristics of NIFNE in Azotobacter vinelandii strains. Implications for the synthesis of the iron-molybdenum cofactor of dinitrogenase. J. Biol. Chem. 270:4432-4437.
27. Shah, V. K., J. R. Allen, N. J. Spangler, and P. W. Ludden. 1994. In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. Purification and characterization of NifB cofactor, the product of the NifB protein. J. Biol. Chem. 269:1154-1158.
28. Shah, V. K., and W. J. Brill. 1977. Isolation of an iron-molybdenum cofactor from nitrogenase. Proc. Natl. Acad. Sci. USA 74:3249-3253.
29. Shah, V. K., and W. J. Brill. 1973. Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacier vinelandii. Biochim. Biophys. Acta 305:445-454.
30. Shah, V. K., J. R. Chisnell, and W. J. Brill. 1978. Acetylene reduction by the iron-molybdenum cofactor from nitrogenase. Biochem. Biophys. Res. Commun. 81:232-236.
31. Shah, V. K., J. Imperial, R. A. Ugalde, P. W. Ludden, and W. J. Brill. 1986. In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. Proc. Natl. Acad. Sci. USA 83:1636-1640.
32. Ugalde, R. A., J. Imperial, V. K. Shah, and W. J. Brill. 1984. Biosynthesis of iron-molybdenum cofactor in the absence of nitrogenase. J. Bacteriol. 159: 888-893.
33. Zheng, L., R. H. White, and D. R. Dean. 1997. Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase. J. Bacteriol. 179:5963-5966.