Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database

Electrophoresis

Volumn 20, Issue 4-5, 1999, Pages 977-983

  Shaw, Allan Christian ^b   Larsen, Martin Røssel ^c   Roepstorff, Peter ^c   Holm, Arne ^a   Christiansen, Gunna ^c   Birkelund, Svend ^c  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b AARHUS UNIVERSITY   (Denmark)

^c NONE

Author keywords

HeLa; Immobilized pH gradient; Make2ddb; Two dimensional gel electrophoresis; World Wide Web

Indexed keywords

AMINO ACIDS; CELL CULTURE; MAPPING; PH; PROTEINS; VIRUSES;

CELL PROTEINS; HELA CELL; HELA CELL LINE; HUMAN ADENOCARCINOMA; IMMOBILIZED PH GRADIENT; MAKE2DDB; PH GRADIENTS; RESEARCH FIELDS; TWO-DIMENSIONAL GEL ELECTROPHORESIS; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS;

LANTHANUM COMPOUNDS;

CELL PROTEIN; CREATINE KINASE; ELONGATION FACTOR 1; EUKARYOTIC TRANSLATION FACTOR; GALECTIN; GAMMA INTERFERON; GLUCOSE REGULATED PROTEIN 78; GLUTATHIONE TRANSFERASE; HEAT SHOCK COGNATE 1; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70; INITIATION FACTOR 5A; KERATIN; LIPOCORTIN 1; PROTEASOME; PROTEIN DISULFIDE ISOMERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; RIBOSOME PROTEIN; T COMPLEX PROTEIN 1; THIOREDOXIN; TRYPTOPHAN TRANSFER RNA LIGASE; UNCLASSIFIED DRUG; VIMENTIN;

AMINO ACID SEQUENCE; COMPUTER PROGRAM; CONFERENCE PAPER; DATA BASE; HOST PARASITE INTERACTION; HUMAN; HUMAN CELL; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0032947531     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(19990101)20:4/5<977::AID-ELPS977>3.0.CO;2-J     Document Type: Conference Paper

References (18)

1. Lundemose, A. G., Birkelund, S., Larsen, P. M., Fey, S. J., Christiansen, C., Infect. Immun. 1990, 58, 2478-2486.
2. Birkelund, S., Bini, L, Palini, V., Sanchez-Campilo, M., Liberatori, S., Clausen, J. D., Østergaard, S., Holm, A., Christiansen, G., Electrophoresis 1997, 18, 563-567.
3. Bravo, R., Celis, J. E., Clin. Chem. 1982, 28, 766-781.
4. Görg, A., Postel, W., Günther, S., Electrophoresis 1988, 9, 531-546.
5. Sanchez, J.-C., Rouge, V., Pisteur, M., Ravier, F., Tonella, L., Moosmayer, M., Wilkins, M. R., Hochstrasser, D. F., Electrophoresis 1997, 18, 324-327.
6. Nawrocki, A., Larsen, M. R., Podtelejnikov, A. V., Jensen, O. N., Mann, M., Roepstorff, P., Görg, A., Fey, S. J., Larsen, P. M., Electrophoresis 1998, 19, 1024-1035.
7. Hoogland, C., Sanchez, J.-C., Tonella, L., Hochstrasser, D. F., Appel, R. D., in: From Genome to Proteome, 3rd Siena 2-D Electrophoresis Meeting 1998, Abstract, p. 91.
8. Appel, R. D., Bairoch, A., Sanchez, J.-C., Vargas, J. R., Golaz, O., Pasquali, C., Ravier, F., Hochstrasser, D. F., Electrophoresis 1996, 17, 540-546.
9. Sanchez, J.-C., Appel, R. D., Golaz, O., Pasquali, C., Ravier, F., Bairoch, A., Hochstrasser, D. F., Electrophoresis 1995, 16, 1131-1151.
10. Rosenfeld, J., Capdevielle, J., Guillemot, J. C., Ferrara, P., Anal. Biochem. 1992, 203, 173-179.
11. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.
12. Gobom, J., Nordhoff, E., Ekman, R., Roepstorff, P., Int. J. Mass Spec. Ion Proc. 1997, 169/170, 153-163.
13. Towbin, H., Staehelin, T., Gordon, J., Proc. Natl. Acad. Sci. USA 1979, 76, 4350-4354.
14. Mann, M., Højrup, P., Roepstorff, P., Biol. Mass Spectrom. 1993, 22, 338-345.
15. Bjellqvist, B., Hughes, G., Pasquali, C., Paquet, N., Ravier, F., Sanchez, J.-C., Frutiger, S., Hochstrasser, D. F., Electrophoresis 1993, 14, 1023-1031.
16. Bjellqvist, B., Basse, B., Olsen, E., Celis, J. E., Electrophoresis 1994, 15, 529-539.
17. Leustek, T., Amir-Shapira, D., Toledo, H., Brot, N., Weissbach, H., Cell. Mol. Biol. 1992, 38, 1-10.
18. Blomberg, A., Blomberg, L., Norbeck, J., Fey, S. J., Larsen, P. M., Larsen, M., Roepstorff, P., Degand, H., Boutry, M., Posch, A., Görg, A., Electrophoresis 1995, 16, 1935-1945.