Accurate quantification of the mRNA of NMDAR1 splice variants measured by competitive RT-PCR

Brain Research Protocols

Volumn 4, Issue 1, 1999, Pages 69-81

  Winkler, Anett ^a   Mahal, Beatrice ^a   Zieglgänsberger, Walter ^a   Spanagel, Rainer ^a  

^a MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

Author keywords

Competitive RT PCR; Gene expression; NMDA receptor; Rat brain; Splice variant

Indexed keywords

GLUTAMATE RECEPTOR; MESSENGER RNA; N METHYL DEXTRO ASPARTIC ACID; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; PROTEIN SUBUNIT;

ACCURACY; ALTERNATIVE RNA SPLICING; ANIMAL TISSUE; ARTICLE; GENE EXPRESSION; IN SITU HYBRIDIZATION; MALE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; RECEPTOR DENSITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0032947444     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(99)00005-7     Document Type: Article

References (20)

1. Anantharam V., Panchal R.G., Wilson A., Kolchine V.V., Treistman S.N., Bayley H. Combinatorial RNA splicing alters the surface charge on the NMDA receptor. FEBS Lett. 305:1992;27-30.
2. Benke D., Wenzel A., Scheuer L., Fritschy J.M., Möhler H. Immuno-biochemical characterization of the NMDA-receptor subunit NR1 in the developing and adult rat brain. J. of Receptor and Signal Transduction Res. 15:1995;393-411.
3. A. Bothwell, G.D. Yancopoulos, F.W. Alt, Methods for cloning and analysis of eucaryotic genes, Jones and Barlett Publishers, 1990.
4. Bouabola M., Legoux P., Pessegue B., Delpetch P., Dumont X., Picchaczyk M., Casellas P., Shire D. Standardization of mRNA titration using a polymerase chain reaction method involving coamplification with multispecific internal control. J. Biol. Chem. 267:1992;21830-21838.
5. Búzas B., Cox B.M. Quantitative analysis of mu and delta opioid receptor gene expression in rat brain and peripheral ganglia using competitive polymerase chain reaction. Neuroscience. 76:1997;479-489.
6. M.J. Dallman, A.C.G. Porter, Semi-quantitative PCR for the analysis of gene expression, in: M.J. McPherson, P. Quirke, G.R. Taylor (Eds.), PCR A Practical Approach, IRL Press, 1992, pp. 215-224.
7. Förster E. An improved general method to generate internal standards for competitive PCR. BioTechniques. 16:1994;18-20.
8. Hollmann M., Boulter J., Maron C., Beasley L., Sullivan J., Pecht G., Heinemann S. Zinc potentiates agonist-induced currents at certain splice variants of the NMDA receptor. Neuron. 10:1993;943-954.
9. Hollmann M., Heinemann S. Cloned glutamate receptors. Ann. Rev. Neurosci. 17:1994;31-108.
10. Laurie D.J., Seeburg P.H. Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14:1994;3180-3194.
11. M.J. McPherson, P. Quirke, G.R. Taylor, PCR: A practical approach, Oxford University Press, 1992.
12. Mori H., Mishina M. Structure and function of the NMDA receptor channel. Neuropharmacology. 34:1995;1219-1237.
13. Moriyoshi K., Masu M., Ishii T., Shigemoto R., Mizuno N., Nakanishi S. Molecular cloning and characterization of the rat NMDA receptor. Nature. 354:1991;31-37.
14. Nakanishi N., Axel R., Schneider N.A. Alternative splicing generates functionally distinct N-methyl-D-aspartate receptors. Proc. Natl. Acad. Sci. USA. 89:1992;8552-8556.
15. G. Paxinos, C. Watson, The rat brain in stereotaxic coordinates, 2nd edn., Academic Press, New York, 1986.
16. J. Putzke, Tierexperimentelle Studien zur Änderung der Genexpression während verschiedener Phasen der Alkoholabhängigkeit, PhD thesis, 1997.
17. J. Sambrook, E.H. Fritsch, T. Maniatis, Molecular Cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, 1989.
18. Standaert D.G., Testa C.M., Young A.B., Penney J.B. Jr. Organization of N-methyl-D-aspartate glutamate receptor gene expression in the basal ganglia of the rat. J. Comp. Neurology. 343:1994;1-16.
19. Sugihara H., Moriyoshi K., Ishii T., Masu M., Nakanishi S. Structures and properties of seven isoforms of the NMDA receptor generated by alternative splicing. Biochem. Biophys. Res. Comm. 185:1992;826-832.
20. Zukin R.S., Bennett M.V.L. Alternatively spliced isoforms of the NMDAR1 receptor subunit. TiNS. 18:1995;306-313.