Calcium currents of embryonic and adult neurons in serum-free culture

Brain Research Bulletin

Volumn 48, Issue 1, 1999, Pages 73-78

  Collings, Melissa A ^a   Brewer, Gregory J ^a   Evans, M Steven ^a  

^a SOUTHERN ILLINOIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adult neurons; B27; Calcium channels; Neurobasal; Neuronal culture; Patch clamp; Voltage sensitive channels

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CALCIUM CURRENT; CELL SURVIVAL; CONTROLLED STUDY; ELECTROPHYSIOLOGY; EMBRYO DEVELOPMENT; MEMBRANE DEPOLARIZATION; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NERVE EXCITABILITY; NERVE FUNCTION; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; RECEPTOR BLOCKING; SYNAPTIC TRANSMISSION;

AGING; ANIMALS; CALCIUM CHANNELS; CALCIUM CHANNELS, L-TYPE; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; ELECTRIC CONDUCTIVITY; EMBRYO; NEURONS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0032946057     PISSN: 03619230     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0361-9230(98)00147-6     Document Type: Article

References (20)

1. Ahmed Z., Walker P.S., Fellows R.E. Properties of neurons from dissociated fetal rat brain in serum-free culture. J. Neurosci. 3:1983;2448-2462.
2. Armstrong C.M., Bezanilla F. Charge movement associated with the opening and closing of the activation gates of the sodium channel. J. Gen. Physiol. 63:1974;533-552.
3. Brewer G.J. Isolation and culture of adult rat hippocampal neurons. J. Neurosci. Meth. 71:1997;145-158.
4. Brewer G.J., Torricelli J.R., Evenge E.K., Price P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal™, a new serum-free medium combination. J. Neurosci. Res. 35:1993;567-576.
5. Campbell L.W., Hao S.Y., Thibault O., Blalock E.M., Landfield P.W. Aging changes in voltage-gated calcium currents in hippocampal CA1 neurons. J. Neurosci. 16:1996;6286-6295.
6. Collings M.A., Evans M.S., Brewer G.J. Calcium currents in embryonic hippocampal neurons are increased compared to juvenile adult hippocampal neurons in culture. Soc. Neurosci. Abstr. 23:1997;1162. (Abstract).
7. Evans M.S., Collings M.A., Brewer G.J. Electrophysiology of embryonic, adult, and aged rat hippocampal neurons in serum-free culture. J. Neurosci. Meth. 79:1997;37-46.
8. Fox A.P., Nowycky M.C., Tsien R.W. Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones. J. Physiol. 394:1987;149-172.
9. Hamill O., Marty A., Neher E., Sakmann B., Sigworth F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391:1981;85-100.
10. 2+ channels by G-protein βgamma subunits. Nature. 380:1996;258-262.
11. Ikeda S.R. Voltage-dependent modulation of N-type calcium channels by G-protein βgamma subunits. Nature. 380:1996;255-258.
12. Karst H.J.M.W.W.J. Low-threshold calcium current in dendrites of the adult rat hippocampus. Neurosci. Lett. 164:1993;154-158.
13. Koike H., Saito H., Matsuki N. Effect of fibroblast growth factors on calcium currents in acutely isolated neuronal cells from rat ventromedial hypothalamus. Neurosci. Lett. 150:1993;57-60.
14. Lorenzon N.M., Foehring R.C. Characterization of pharmacologically identified voltage-gated calcium channel currents in acutely isolated rat neocortical neurons. II. Postnatal development. J. Neurophysiol. 73:1995;1443-1451.
15. McCobb D.P., Best P.M., Beam K.G. Development alters the expression of calcium currents in chick limb motoneurons. Neuron. 2:1989;1633-1643.
16. Sah D.W.Y., Ray J., Gage F.H. Regulation of voltage- and ligand-gated currents in rat hippocampal progenitor cells in vitro. J. Neurobiol. 32:1996;95-110.
17. Strauss O., Mergler S., Wiederholt M. Regulation of L-type calcium channels by protein tyrosine kinase and protein kinase C in cultured rat and human retinal pigment epithelial cells. FASEB J. 11:1997;859-867.
18. Thibault O., Landfield P.W. Increase in single L-type calcium channels in hippocampal neurons during aging. Science. 272:1996;1017-1020.
19. Thompson S.M., Wong R.K.S. Development of calcium current subtypes in isolated rat hippocampal pyramidal cells. J. Physiol. 439:1991;671-689.
20. Tsien R.W., Lipscombe D., Madison D.V., Bley K.R., Fox A.P. Multiple types of neuronal calcium channels and their selective modulation. Trends Neurosci. 11:1988;431-438.