Effects of site-directed mutagenesis of Escherichia coli heat-labile enterotoxin on ADP-ribosyltransferase activity and interaction with ADP- ribosylation factors

Infection and Immunity

Volumn 67, Issue 1, 1999, Pages 259-265

  Stevens, Linda A ^a   Moss, Joel ^a,c   Vaughan, Martha ^a   Pizza, Mariagrazia ^b   Rappuoli, Rino ^b  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b CHIRON VACCINES   (Italy)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DERIVATIVE; ADENOSINE DIPHOSPHATE RIBOSE; ADENOSINE DIPHOSPHATE RIBOSYLAGMATINE; ADENOSINE DIPHOSPHATE RIBOSYLATION FACTOR; AMINO ACID; ARGININE; CHOLERA TOXIN; DITHIOTHREITOL; ESCHERICHIA COLI ENTEROTOXIN; GUANINE NUCLEOTIDE BINDING PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN SUBUNIT; SERINE; TRYPSIN; TYROSINE; UNCLASSIFIED DRUG; VALINE;

ADENOSINE DIPHOSPHATE RIBOSYLATION; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ESCHERICHIA COLI;

EID: 0032945714     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.67.1.259-265.1999     Document Type: Article

References (36)

1. Barbieri, J. T., L. M. Mende-Mueller, R. Rappuoli, and R. J. Collier. 1989. Photolabeling of Glu-129 of the S-1 subunit of pertussis toxin with NAD. Infect. Immun. 57:3549-3554.
2. Birnbaumer, L., R. Mattera, A. Yatani, J. Codina, A. M. J. VanDongen, and A. M. Brown. 1990. Recent advances in the understanding of multiple roles of G proteins in coupling of receptors to ionic channels and other effectors, p. 225-266. In J. Moss and M. Vaughan (ed.), ADP-ribosylating toxins and G proteins: insights into signal transduction. American Society for Microbiology, Washington, D.C.
3. Carroll, S. F., and R. J. Collier. 1984. NAD binding site of diphtheria toxin: identification of a residue within the nicotinamide subsite by photochemical modification with NAD. Proc. Natl. Acad. Sci. USA 81:3307-3311.
4. Carroll, S. F., and R. J. Collier. 1987. Active site of Pseudomonas aeruginosa exotoxin A. Glutamic acid 553 is photolabeled by NAD and shows functional homology with glutamic acid 148 of diphtheria toxin. J. Biol. Chem. 262: 8707-8711.
5. Cosson, P., and F. Letourneur. 1997. Coatomer (COPI)-coated vesicles: role in intracellular transport and protein sorting. Curr. Opin. Cell Biol. 9:484-487.
6. Domenighini, M., C. Magagnoli, M. Pizza, and R. Rappuoli. 1994. Common features of the NAD-binding and catalytic site of ADP-ribosylating toxins. Mol. Microbiol. 14:41-50.
7. Douce, G., C. Turcotte, I. Cropley, M. Roberts, M. Pizza, M. Domenghini, R. Rappuoli, and G. Dougan. 1995. Mutants of Escherichia coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic, mucosal adjuvants. Proc. Natl. Acad. Sci. USA 92:1644-1648.
8. Fontana, M. R., R. Manetti, V. Giannelli, C. Magagnoli, A. Marchini, R. Olivieri, M. Domenighini, R. Rappuoli, and M. Pizza. 1995. Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit. Infect. Immun. 63:2356-2360.
9. Giannelli, V., M. R. Fontana, M. M. Giuliani, D. Guangcai, R. Rappuoli, and M. Pizza. 1997. Protease susceptibility and toxicity of heat-labile enterotoxins with a mutation in the active site or in the protease-sensitive loop. Infect. Immun. 63:331-334.
10. 1 fragment of cholera toxin. J. Infect. Dis. 139:674-680.
11. Jung, M., I. Just, J. Damme, J. Vandekerckhove, and K. Aktories. 1993. NAD-binding site of the C3-like ADP-ribosyltransferase from Clostridium limosum. J. Biol. Chem. 268:23215-23218.
12. Kahn, R. A., and A. G. Gilman. 1984 Purification of a protein cofactor required for ADP-ribosylation of the stimulatory regulatory component of adenylate cyclase by cholera toxin. J. Biol. Chem. 259:6228-6234.
13. Lee, C.-M., P. P. Chang, S.-C. Tsai, S.-C. R. Adamik, S. R. Price, B. C. Kunz, J. Moss, E. M. Twiddy, and R. K. Holmes. 1991. Activation of Escherichia coli heat-labile enterotoxins by native and recombinant adenosine diphosphate-ribosylation factors, 20-kD guanine nucleotide-binding proteins. J. Clin. Invest. 87:1780-1786.
14. Lobet, Y., C. W. Cluff, and W. Cieplak, Jr. 1991. Effect of site-directed mutagenic alterations on ADP-ribosyltransferase activity of the A subunit of Escherichia coli heat-labile enterotoxin. Infect. Immun. 59:2870-2879.
15. Magagnoli, C., R. Manetti, M. R. Fontana, V. Giannelli, M. M. Giuliani, R. Rappuoli, and M. Pizza. 1996. Mutations in the A subunit affect yield, stability, and protease sensitivity of nontoxic derivatives of heat-labile enterotoxin. Infect. Immun. 64:5434-5438.
16. McDonald, L. J., and J. Moss. 1993. Stimulation by nitric oxide of an NAD linkage to glyceraldehyde-3-phosphate dehydrogenase. Proc. Natl. Acad. Sci. USA 90:6238-6241.
17. McDonald, L. J., and J. Moss. 1993. Nitric oxide-independent, thiol-associated ADP-ribosylation inactivates aldehyde dehydrogenase. J. Biol. Chem. 268:17878-17882.
18. Mekalanos, J. J., R. J. Collier, and W. R. Romig. 1979. Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. J. Biol. Chem. 254:5855-5861.
19. Merritt, E. A., S. E. Pronk, T. K. Sixma, K. H. Kalk, B. A. M. Van Zanten, and W. G. J. Hol. 1994. Structure of partially-activated E. coli heat-labile enterotoxin (LT) at 2.6 Å resolution. FEBS Lett. 337:88-92.
20. Merrit, E. A., S. Sarfaty, M. Pizza, M. Domenighini, R. Rappuoli, and W. G. J. Hol. 1995. Mutation of a buried residue causes loss of activity hut no conformational change in the heat-labile enterotoxin of Escherichia coli. Nat. Struct. Biol. 2:269-272.
21. Moss, J., J. C. Osborne Jr., P. H. Fishman, S. Nakaya, and D. C. Robertson. 1981. Escherichia coli heat-labile enterotoxin: ganglioside specificity and ADP-ribosyltransferase activity. J. Biol. Chem. 256:12861-12865.
22. Moss, J., S. J. Stanley, M. Vaughan, and T. Tsuji. 1993. Interaction of ADP-ribosylation factor with Eschenchia coli enterotoxin that contains an inactivating lysine 112 substitution. J. Biol. Chem. 268:6383-6387.
23. Moss, J., and M. Vaughan. 1988. ADP-ribosylation of guanyl nucleotide-binding regulatory proteins by bacterial toxins. Adv. Enzymol. Relat. Areas Mol. Biol. 61:303-379.
24. Moss, J., and M. Vaughan. 1995. Structure and function of ARF proteins: activators of cholera toxin and critical components of intracellular vesicular transport processes. J. Biol. Chem. 270:12327-12330.
25. Noda, M., S.-C. Tsai, R. Adamik, J. Moss, and M. Vaughan. 1990. Mechanism of cholera toxin activation by a guanine nucleotide-dependent 19 kDa protein. Biochim. Biophys. Acta 1034:195-199.
26. Pizza, M., M. Domenighini, W. Hol, V. Giannelli, M. R. Fontana, M. M. Giuliani, C. Magagnoli, S. Peppoloni, R. Manetti, and R. Rappuoli. 1994. Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis. Mol. Microbiol. 14:51-60.
27. Pizza, M., M. R. Fontana, M. M. Giuliani, M. Domenighini, C. Magagnoli, V. Giannelli, D. Nucci, W. Hol, R. Manetti, and R. Rappuoli. 1994. A genetically detoxified derivative of heat-labile Escherichia coli enterotoxin induces neutralizing antibodies against the A subunit. J Exp. Med. 180: 2147-2153.
28. Price, S. R., C. F. Welsh, R. S. Haun, S. J. Stanley, J. Moss, and M. Vaughan. 1992. Effects of phospholipid and GTP on recombinant ADP-ribosylation factors (ARFs). Molecular basis for differences in requirements for activity of mammalian ARFs. J. Biol. Chem. 267:17766-17772.
29. Rothman, J. E. 1994. Mechanisms of intracellular protein transport. Nature 372:55-63.
30. Sixma, T. K., S. E. Pronk, K. H. Kalk, E. S. Wartna, B. A. M. vanZanten, B. Witholt, and W. G. J. Hol. 1991. Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli. Nature 351:371-377.
31. Tsai, S.-C., R. Adamik, J. Moss, and K. Aktories. 1988. Separation of the 24 kDa substrate for Botulinum C3 ADP-ribosyltransferase and the cholera toxin ADP-ribosylation factor. Biochem. Biophys. Res. Commun. 152:957-961.
32. Tsai, S.-C., M. Noda, R. Adamik, P. P. Chang, H.-C. Chen, J. Moss, and M. Vaughan. 1988. Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J. Biol. Chem. 263:1768-1772.
33. Tsuchiya, M., S. R. Price, S.-C. Tsai, J. Moss, and M. Vaughan. 1991. Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells. J. Biol. Chem. 266:2772-2777.
34. Tsuji, T., T. Inoue, A. Miyama, K. Okamoto, T. Honda, and T. Miwatani. 1990. A single amino acid substitution in the A subunit of Escherichia coli enterotoxin results in a loss of its toxic activity. J. Biol. Chem. 265:22520-22525.
35. Van den Akker, F., E. A. Merritt, M. G. Pizza, M. Domenighini, R. Rappuoli, and W. G. J. Hol. 1995. The Arg7Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit. Biochemistry 34:10996-11004.
36. Van den Akker, F., M. Pizza, R. Rappuoli, and W. G. Hol. 1997. Crystal structure of a non-toxic mutant of heat-labile enterotoxin, which is a potent mucosal adjuvant. Protein Sci. 6:2650-2654.