Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor

Enzyme and Microbial Technology

Volumn 24, Issue 8-9, 1999, Pages 507-513

  Serralheiro, M L M ^a   Prazeres, D M F ^a   Cabral, Joaquim M S ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Chymotrypsin; Dipeptide synthesis; Membrane reactor; Precipitation; Process integration; Reversed micelles

Indexed keywords

BIOCONVERSION; BIOLOGICAL MEMBRANES; BIOREACTORS; BIOSYNTHESIS; CATALYST SELECTIVITY; ENCAPSULATION; ESTERIFICATION; HYDROLYSIS; PRECIPITATION (CHEMICAL); PROTEINS;

CHYMOTRYPSIN; CONTINUOUS PRODUCTION; DIPEPTIDE; OPERATIONAL STABILITY; PROCESS INTEGRATION; REVERSED MICELLAR MEMBRANE REACTOR;

CONTINUOUS CELL CULTURE;

CHYMOTRYPSIN A; DIPEPTIDE;

ARTICLE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; FLOW RATE; MEMBRANE REACTOR; MICELLE; PEPTIDE SYNTHESIS; PRECIPITATION; TECHNIQUE;

EID: 0032944832     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00141-0     Document Type: Article

References (21)

1. Oyama, K. and Kihara, K. A new horizon for enzyme technology. Chemtech 1984, 2, 100-105
2. Luthi, P. and Luisi, P. L. Enzymatic synthesis of hydrocarbon soluble peptides with reverse micelles. J. Am. Chem. Soc. 1984, 106, 7285-7286
3. Shield, J. W., Ferguson, H. D., Gleason, K. K., and Hatton, T. A. Enzymatic reactions in reversed micelles at low solubilized water concentrations. In: Biocatalysis and Biomimetics (Burrington, J. D. and Clark, D. S., Eds.). ACS Symposium Series 392, ACS, Washington DC, 1989, 90-103
4. 2 in reversed micelles. Enzyme Microb. Technol. 1992, 14, 117-124
5. Chang, P. S., Rhee, J. S., and Kim, J. J. Continuous glycerolysis of olive oil by Chromobacterium viscosum lipase immobilized on liposome in reversed micelles. Biotechnol. Bioeng. 1991, 38, 1159-1165
6. Chiang, C.-L. and Tsai, S.-W. Application of a recycle dialysis system in a reversed micellar reactor. J. Chem. Technol. Biotechnol. 1992, 54, 27-32
7. Prazeres, D. M. F., Garcia, F. A. P., and Cabral, J. M. S. An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media. Biotechnol. Bioeng. 1993, 41, 761-770
8. Prazeres, D. M. F., Garcia, F. A. P., and Cabral, J. M. S. Continuous lipolysis in a reversed micellar membrane bioreactor. Bioprocess Eng. 1994, 10, 21-27
9. Serralheiro, M. L. M., Prazeres, D. M. F., and Cabral, J. M. S. Dipeptide synthesis and separation in a reversed micellar membrane reactor. Enzyme Microb. Technol. 1994, 16, 1064-1073
10. Prazeres, D. M. F. and Cabral, J. M. S. Enzymatic membrane reactors and their applications. Enzyme Microb. Technol. 1994, 16, 1-13
11. Kullmann, W. Enzymatic Peptide Synthesis. CRC Press, Inc. Boca Raton, FL 1987
12. Serralheiro, M. L. M., and Cabral, J. M. S. Application of fractional factorial design to the study of enzymatic dipeptide synthesis in reverse micelles. In: Biocatalysis in Non-Conventional Media (Tramper, J., Vermue, M. H., Beeftnik, H. H., and von Stockar, U., Eds.). Elsevier, Amsterdam, 1992, 725-732
13. Cheryan, M. Ultrafiltration Handbook. Technomic Publishing Company, Inc. Lancaster, PA
14. Serralheiro, M. L. M., and Cabral, J. M. S. Influence of long chain alcohol and chemical modification on the microencapsulated α-chymotrypsin stability. In: Stability and Stabilization of Enzymes (van den Tweel, W. J. J., Harder, A., and Buitelaar, R. M., Eds.). Elsevier, Amsterdam, 1993, 481-488
15. Florsheimer, A., Kula, M.-R., Schutz, H.-J., and Wandrey, C. Continuous production of kyotorphin. Biotechnol. Bioeng. 1989, 33, 1400-1405
16. α-protecting groups of amino acids. Biocatalysis 1994, 8, 289-307
17. Schwarz, A, Wandrey, C., Steinke, D., and Kula, M. R. A two-step enzymatic synthesis of dipeptide. Biotechnol. Bioeng. 1992, 39, 132-140
18. Herrmann, G., Schwarz, A., Wandrey, C., Kula, M.-R., Knaup, G., Dreuz, K. H., and Berndt, A. A scale-up of enzymatic peptide synthesis in an enzyme membrane reactor. Biotechnol. Appl. Biochem. 1991, 13, 346-353
19. Nagayasu, T., Miyanaga, M., Tanaka, T., Sakiyama, T., and Nakanishi, K. Synthesis of aspartame precursor with an immobilized thermolysin in tert-amyl alcohol. Biotechnol. Bioeng. 1994, 43, 1118-1123
20. Richards, A. O., Gill, I. S., and Vulfson, E. N. Continuous enzymatic production of oligopeptides: synthesis of an enkephalin pentapeptide in a multistage bioreactor. Enzyme Microb. Technol. 1993, 15, 928-935
21. Wiseman, A. Stabilization of enzymes. In: Topics in Enzyme and Fermentation Technology Vol. 2 (Wiseman, A., Ed.). Ellis-Horwiid Publishers, 1978, 280-303