The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland ductal cell line HSG cells

Oral Diseases

Volumn 5, Issue 2, 1999, Pages 104-110

  Morimoto, Y ^a   Morimoto, H ^a   Kobayashi, S ^a   Ohba, T ^a   Haneji, Tatsuji ^a  

^a KYUSHU DENTAL COLLEGE   (Japan)

Author keywords

Apoptosis; Calyculin A; HSG cells; Okadaic acid; Protein phosphatase; Salivary gland

Indexed keywords

CALYCULIN A; CYCLOHEXIMIDE; DACTINOMYCIN; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE 1; PHOSPHOPROTEIN PHOSPHATASE 2A; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL LINE; CHROMATIN CONDENSATION; CONTROLLED STUDY; CYTOTOXICITY; DEPHOSPHORYLATION; DOSE TIME EFFECT RELATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; REGULATORY MECHANISM; SALIVARY GLAND; SUBMANDIBULAR GLAND;

EID: 0032944712     PISSN: 1354523X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1601-0825.1999.tb00073.x     Document Type: Article

References (24)

1. Bialojan C, Takai A (1988). Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Biochem J 256: 283-290.
2. Bøe R, Gjertsen BT, Vintermyr OK et al (1991). The protein phosphatase inhibitor okadaic acid induces morphological changes typical of apoptosis in mammalian cells. Exp Cell Res 195: 237-246.
3. Cohen P (1989). The structure and regulation of protein phosphatases. Annu Rev Biochem 58: 453-508.
4. Cohen P, Holmes CFB, Tsukitani Y (1990). Okadaic acid: A new probe for the study of cellular regulation. Trends Biochem Sci 15: 98-102.
5. Enari M, Sakahira H, Yokoyama H et al (1998). A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391: 43-50.
6. 2-phase to induce premature mitosis in HeLa cells. Exp Cell Res 227: 165-169.
7. Giese G, Wiegers W, Kubbie M et al (1995). Okadaic, acid co-induces vimentin expression and cell cycle arrest in MPC-11 mouse plasmacytoma cells. J Cell Physiol 163: 145-154.
8. Gjertsen BT, Cressey LI, Ruchaud S et al (1994). Multiple apoptotic death types triggered through activation of separate pathways by cAMP and inhibitors of protein phosphatases in one (IPC leukemia) cell line. J Cell Sci 107: 3363-3377.
9. Hoffman MP, Kibbey MC, Letterio JJ et al (1996). Role of laminin-1 and TGF-beta3 in acinar differentiation of a human submandibular gland cell line (HSG). J Cell Sci 109: 2013-2021.
10. Kerr JFR, Wyllie AH, Currie AR (1972). Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 219-257.
11. Kiguchi K, Glesne D, Chubb CH et al (1994). Differential induction of apoptosis in human breast tumor cells by okadiac acid and related inhibitors of protein phosphatases 1 and 2A. Cell Growth Differ 5: 995-1004.
12. Morimoto Y, Ohba T, Kobayashi S et al (1997). The protein phosphatase inhibitors okadaic acid and calyculin. A induce apoptosis in human osteoblastic cells. Exp Cell Res 230: 181-186.
13. Ohoka Y, Nakai Y, Mukai M et al (1993). Okadiac acid inhibits glucocorticoid-induced apoptosis in T cell hybridomas at its late stage. Biochem Biophys Res Commun 197: 916-921.
14. Sato M, Hayashi Y, Yoshida H et al (1984). Search for specific markers of neoplastic epithelial duct and myoepithelial cell lines established from human salivary gland and characterization of their growth in vitro. Cancer 54: 2959-2967.
15. Shirakawa S, Mochizuki H, Kobayashi S et al (1996). Immunohistochemical and immunoblotting identification of protein phosphatase 1γ1 in rat salivary glands. FEBS Lett 393: 57-59.
16. Shirasuna K, Sato M, Miyazaki T (1981). A neoplastic epithelial duct cell line established from an irradiated human salivary gland. Cancer 48: 745-752.
17. Song Q, Lavin MF (1993). Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis. Biochem Biophys Res Commun 190: 47-55.
18. Takenouchi T, Munekata E (1995). Trophic effect of substance P and β-amyloid peptide on dibutyryl cyclic AMP-differentiated human leukemic (HL-60) cells. Life Sci 56: 479-484.
19. Takuma T, Ichida T, Okumura K et al (1993). Okadaic acid inhibits amylase exocytosis from parotid acini stimulated by cyclic AMP. FEBS Lett 323: 145-150.
20. Weil M, Jacobson MD, Coles HSR et al (1996). Constitutive expression of the machinery for programmed cell death. J Cell Biol 133: 1053-1059.
21. 2 Modulation by the phosphatase inhibitors okadaic acid and calyculin A. Exp Cell Res 221: 395-403.
22. Wu AJ, Chen ZJ, Tsokos M et al (1996). Interferon-γ induced cell death in a cultured human salivary gland cell line. J Cell Physiol 167: 297-304.
23. Wyllie AH (1980). Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555-556.
24. Yagil C, Michaeli Y, Zahucej G (1985) Compensatory proliferative response of the rat submandibular salivary gland to unilateral extirpation. Virchows Arch B Cell Pathol 49: 83-92.