Primer mixture enhances PCR detection of Mycoplasma/Acholeplasma contaminants in cell cultures [1]

In Vitro Cellular and Developmental Biology - Animal

Volumn 35, Issue 1, 1999, Pages 1-3

  Tang, J ^a   Hu, M ^a   Lee, S ^a   Roblin, R ^a  

^a AMERICAN TYPE CULTURE COLLECTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACHOLEPLASMA; BACTERIUM CONTAMINATION; BACTERIUM IDENTIFICATION; CELL CULTURE; LETTER; MYCOPLASMA; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ACHOLEPLASMA; BACTERIA (MICROORGANISMS); MYCOPLASMA;

EID: 0032942412     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-999-0033-5     Document Type: Letter

References (16)

1. 1. S. Arai R. Harasawa T. Ohno M. Takeuchi K. Hikizi N. Kobayashi I. Lee K. Kato H. Takahashi I. Okamoto T. Furudera 1994 Comparative studies to detect mycoplasma contamination in bioindustrial materials, for validating standard method Int. Org. Mycoplasmol. Lett. 3 48 49 Arai, S.; Harasawa, R.; Ohno, T.; Takeuchi, M.; Hikizi, K.; Kobayashi, N.; Lee, I.; Kato, K.; Takahashi, H.; Okamoto, I.; Furudera, T. Comparative studies to detect mycoplasma contamination in bioindustrial materials, for validating standard method. Int. Org. Mycoplasmol. Lett. 3:48–49; 1994.
2. 2. M. F. Barile S. Rottem 1993 Mycoplasmas in cell cultures I. Kahane A. Adoni Rapid diagnosis of mycoplasmas Plenum Press New York 155 193 Barile, M. F.; Rottem, S. Mycoplasmas in cell cultures. In: Kahane, I.; Adoni, A., ed. Rapid diagnosis of mycoplasmas. New York: Plenum Press; 1993:155–193.
3. 3. W. M. Berthold E. Grashoff H. Pfutzner U. Schubert H. Hauser 1994 Detection of mycoplasma contaminations by the polymerase chain reaction Cytotechnology 16 67 77 7765790 10.1007/BF00754609 Berthold, W. M.; Grashoff, E.; Pfutzner, H.; Schubert, U.; Hauser, H. Detection of mycoplasma contaminations by the polymerase chain reaction. Cytotechnology 16:67–77; 1994.
4. 4. G. Boolske 1988 Survey of mycoplasma infections in cell cultures and a comparison of detection methods Zentralbl. Bakteriol. Mikrobiol. Hyg. 269 331 340 Boolske, G. Survey of mycoplasma infections in cell cultures and a comparison of detection methods. Zentralbl. Bakteriol. Mikrobiol. Hyg. 269:331–340; 1988.
5. 5. O. Dussurget D. Roulland-Dussoix 1994 Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera Appl. Environ. Microbiol. 60 953 959 8161186 1:CAS:528:DyaK2cXjtFKrsbo%3D Dussurget, O.; Roulland-Dussoix, D. Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera. Appl. Environ. Microbiol. 60:953–959; 1994.
6. 6. R. Harasawa H. Mizusawa T. Nozama K. Asada I. Ito 1993 Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S–23S rRNA intergenic spacer regions Res. Microbiol. 144 489 493 7910696 10.1016/0923-2508(93)90057-9 1:CAS:528:DyaK2cXitFantLo%3D Harasawa, R.; Mizusawa, H.; Nozama, T.; Asada, K.; Ito, I. Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S–23S rRNA intergenic spacer regions. Res. Microbiol. 144:489–493; 1993.
7. 7. R. Harasawa 1995 Nested PCR: application to the detection of mycoplasmas S. Razin J. G. Tully Molecular and diagnostic procedures in mycoplasmology Vol. II. A4 Academic Press London Harasawa, R. Nested PCR: application to the detection of mycoplasmas. In: Razin, S.; Tully, J. G., ed. Molecular and diagnostic procedures in mycoplasmology Vol. II. A4. London: Academic Press; 1995.
8. 8. R. J. Hay M. L. Macy T. R. Chen 1989 Mycoplasma infection of cultured cells Nature 339 487 488 2725683 10.1038/339487a0 1:STN:280:BiaB283gvFA%3D Hay, R. J.; Macy, M. L.; Chen, T. R. Mycoplasma infection of cultured cells. Nature 339:487–488; 1989.
9. 9. M. Hu C. Buck D. Jacobs G. Paulino H. Khouri 1995 Application of PCR for detection and identification of mycoplasma contamination in virus stocks In Vitro Cell. Dev. Biol. 31 710 715 1:CAS:528:DyaK2MXpvF2isb8%3D Hu, M.; Buck, C.; Jacobs, D.; Paulino, G.; Khouri, H. Application of PCR for detection and identification of mycoplasma contamination in virus stocks. In Vitro Cell. Dev. Biol. 31:710–715; 1995.
10. 10. G. J. McGarrity H. Kotani 1985 Cell culture mycoplasmas S. Razin M. Barile The Mycoplasmas. Vol. 4 Academic Press New York 353 390 McGarrity, G. J.; Kotani, H. Cell culture mycoplasmas. In: Razin, S.; Barile, M., ed. The Mycoplasmas. Vol. 4. New York: Academic Press; 1985:353–390.
11. 11. G. J. McGarrity H. Kotani 1985 Cell culture mycoplasmas S. Razin M. Barile The Mycoplasmas. Vol. 4 Plenum Press New York 155 193 McGarrity, G. J.; Kotani, H. Cell culture mycoplasmas. In: Razin, S.; Barile, M., ed. The Mycoplasmas. Vol. 4. New York: Plenum Press; 1985:155–193.
12. 12. G. J. McGarrity H. Kotoni G. H. Butler 1992 Mycoplasmas and tissue culture cells J. Maniloff R. N. McElhaney L. R. Finch J. B. Baseman Mycoplasmas, molecular biology and pathogenesis American Society for Microbiology Washington, D.C. 445 454 McGarrity, G. J.; Kotoni, H.; Butler, G. H. Mycoplasmas and tissue culture cells. In: Maniloff, J.; McElhaney, R. N.; Finch, L. R.; Baseman, J. B., ed. Mycoplasmas, molecular biology and pathogenesis. Washington, D.C.: American Society for Microbiology; 1992:445–454.
13. 13. T. Nakagawa T. Uemori K. Asada I. Kato R. Harasawa 1992 Acholeplasma laidlawii has tRNA genes in the 16S–23S spacer of the rRNA operon J. Bacteriol. 174 8163 8165 1281149 1:CAS:528:DyaK3sXit1Kqu7w%3D Nakagawa, T.; Uemori, T.; Asada, K.; Kato, I.; Harasawa, R. Acholeplasma laidlawii has tRNA genes in the 16S–23S spacer of the rRNA operon. J. Bacteriol. 174:8163–8165; 1992.
14. 14. S. Razin 1994 DNA probes and PCR in diagnosis of mycoplasma infections Mol. Cell. Probes 8 497 511 7700272 10.1006/mcpr.1994.1071 1:CAS:528:DyaK2MXivV2ksbk%3D Razin, S. DNA probes and PCR in diagnosis of mycoplasma infections. Mol. Cell. Probes 8:497–511; 1994.
15. 15. G. Rawadi O. Dussurget 1995 Advances in PCR-based detection of mycoplasmas contaminating cell cultures PCR Methods Appl. 4 199 208 8574187 1:CAS:528:DyaK2MXktVSltr4%3D Rawadi, G.; Dussurget, O. Advances in PCR-based detection of mycoplasmas contaminating cell cultures. PCR Methods Appl. 4:199–208; 1995.
16. 16. C. C. Uphoff S. Brauer D. Grunicke S. M. Gignac R. A. F. MacLeod H. Quentmeier K. Steube M. Tummler M. Vogas B. Wagner H. G. Drexler 1992 Sensitivity and specificity of five different mycoplasma detection assays Leukemia 6 335 341 1375305 1:STN:280:By2B28bltlU%3D Uphoff, C. C.; Brauer, S.; Grunicke, D.; Gignac, S. M.; MacLeod, R. A. F.; Quentmeier, H.; Steube, K.; Tummler, M.; Vogas, M.; Wagner, B.; Drexler, H. G. Sensitivity and specificity of five different mycoplasma detection assays. Leukemia 6:335–341; 1992.