High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase

Journal of Bioscience and Bioengineering

Volumn 87, Issue 4, 1999, Pages 456-462

  Takimoto, Akio ^a   Yagi, Shigeo ^a   Mitsushima, Kenji ^a  

^a SHIONOGI AND CO LTD   (Japan)

Author keywords

Cephalosporin C deacetylase, Bacillus subtilis, Escherichia coli, production, purification, properties

Indexed keywords

7 AMINOCEPHALOSPORANIC ACID; CEFALOGLYCIN; CEFALOTIN; CEFAPIRIN; CEPHALOSPORIN C; CEPHALOSPORIN C DEACETYLASE; DEACETYL 7 AMINOCEPHALOSPORANIC ACID; DEACETYLASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; COLUMN CHROMATOGRAPHY; ENZYME PURIFICATION; GENE ISOLATION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; STRUCTURAL GENE;

BACILLUS SUBTILIS; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032936748     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(99)80094-2     Document Type: Article

References (20)

1. 1. O'Callaghain, C. H., Sykes, R. B., Ryan, D. M., Foord, R. D., and Muggleton, P. W.: Cefuroxime-A new cephalosporin antibiotic. J. Antibiot., 29, 29-37 (1976).
2. 2. Totsuka, K., Shimizu, K., Konishi, M., and Yamamoto, S.: Metabolism of S-1108, a new oral cephem antibiotic, and metabolic profiles of its metabolites in humans. Antimicrob. Agent Chemother., 36, 757-761 (1992).
3. 3. Takimoto, A., Mitsushima, K., Yagi, S., and Sonoyama, T.: Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis. J. Ferment. Bioeng., 77, 17-22 (1994).
4. 4. Mitsushima, K., Takimoto, A., Sonoyama, T., and Yagi, S.: Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis. Appl. Environ. Microbiol., 61, 2224-2229 (1995).
5. 5. Twigg, A. J. and Sherratt, D.: Trans-complementable copy-number mutants of plasmid ColE1. Nature (London), 283, 216-218 (1980).
6. 6. Gold, L,: Post-translational regulatory mechanisms in Escherichia coli. Ann. Rev. Biochem., 57, 199-233 (1988).
7. 7. Shine, J. and Dalgarno, L.: The 3′-terminal sequence of E. coli 16S ribosomal RNA: complementary to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA, 71, 1342-1346 (1974).
8. 8. Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989).
9. 9. Hanahan, D.: Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol., 166, 557-580 (1983).
10. 10. Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463-5467 (1977).
11. 11. Messing, J., Crea, R., and Seeburg, P. H.: A system for shotgun DNA sequencing. Nucl. Acids Res., 9, 309-321 (1981).
12. 12. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227, 680-685 (1970).
13. 13. Simpson, R. J., Neuberger, M. R., and Liu, T. Y.: Complete amino acid analysis of proteins from a single hydrolysate. J. Biol. Chem., 251, 1936-1940 (1976).
14. 14. Herman, A., Boer, D. E., Hui, A., Comstock, L. J., Wong, E., and Yasser, M.: Portable Shine-Dalgarno regions: a system for a systematic study of defined alterations of nucleotide sequences within E. coli ribosome binding sites. DNA, 2, 231-235 (1983).
15. 15. Shepard, H. M., Yelverton, E., and Goeddel, D.: Increased synthesis in E. coli of fibroblast and leukocyte interferons through alterations in ribosome binding sites. DNA, 1, 125-131 (1982).
16. 16. Marquis, D. M., Smolec, J. M., and Katz, D. H.: Use of a portable ribosome-binding site for maximizing expression of a eukaryotic gene in Escherichia colt. Gene, 42, 175-183 (1986).
17. 17. Sato, N., Hayami, T., Murata, K., Watanabe, K., Kariya, Y., Sakaguchi, M., Kimura, S., Nonaka, M., and Kimura, A.: Expression of tuna growth hormone cDNA in Escherichia coli. Appl. Microbiol. Biotechnol., 30, 153-159 (1989).
18. 18. Zuker, M. and Stiegler, P.: Optional computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucl. Acids Res., 9, 133-148 (1981).
19. 19. Jensen, E. B. and Carlsen, S.: Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose, acetate, and salts. Biotechnol. Bioeng., 36, 1-11 (1990).
20. 20. Fieschko, J. and Ritch, T.: Production of human alpha consensus interferon in recombinant Escherichia coli. Chem. Eng. Commun., 45, 229-240 (1986).